落叶松人工林地位指数级导向曲线模型的研究

本文用约1000块样地资抖,系统地研究了黑龙江省落叶松人工林的地位指数级导向曲线模型。通过林业统计分析软件Statistica6.0对各落叶松人工林数据进行拟合、分析比较,结果为：从拟合精度、曲线走向与散点分布趋势、残差分布等综合考虑,舒马切尔曲线函数比较适合落叶松人工林地位指数级导向曲线模型的拟合。     

欧盟共同农业政策面临的挑战及最新改革措施

共同农业政策作为联系欧盟成员国农业的纽带,在经历2003年的改革后,虽然在价格补贴、农村发展等方面取得一定的成效,但仍然面临财政负担、食品安全、新成员国加入等来自内外部的挑战和压力.为应对这些挑战和压力,欧盟进行了降低政策价格、微调配额等一系列的最新改革.     

桃新品种‘乾兴银凤’

 ‘乾兴银凤’桃是从日本引进品种中选出的优良品种, 经品种比较试验和多点区域试验, 表现丰产, 抗逆, 不裂果等特性。     

提高新红星苹果品质的栽培技术

前些年新红星等元帅系短枝型以其丰产易管的优点,受到广大果农的欢迎,大大增加果农的收益,也使许多果农管理粗放,造成新红星口味淡、色泽差。但随着果品总量的增加和人民生活水平的提高,对苹果的质量提出了更高的要求,所以我们采取以下栽培管理技术,取得了较好效果,改善了红星苹果的品质。1、加强土肥水管理由于许多农民片面追求产量,大量偏施氮肥,使果实个头小,风味淡和着色差问题越来越     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

植物寄生线虫效应蛋白调控寄主防卫反应分子机制研究进展

在植物寄生线虫与寄主互作过程中,线虫分泌器官如食道腺细胞分泌的效应蛋白在寄主细胞壁修饰和调控寄主免疫反应以及取食位点形成和维护中发挥着关键作用。解析植物寄生线虫关键效应蛋白的功能及其与寄主互作机制将为探索植物寄生线虫防控新策略提供重要的理论基础。本文从效应蛋白降解寄主细胞壁、调控寄主基础免疫反应、诱导免疫反应机制和介导翻译后修饰调控寄主免疫反应以及植物激素代谢途径的调控机制等方面进行了概述。     

Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy.     

不同处理方法对重楼种子萌发的Meta分析

应用Meta分析方法,通过中英文数据库筛选了1990—2019年关于重楼(Paris polyphylla)种子萌发的文献24篇,得到78个独立试验组,分析温度、机械、生长素、复合处理对重楼种子萌发的影响,并探讨较高海拔下重楼种子繁育的可能性。结果表明,恒温处理促进重楼种子萌发是一种简单可靠的方法,恒温、变温、沙藏方式的交互处理能够有效提高重楼种子发芽率(相对发芽率为0.61)。生长素使用不当会抑制重楼种子萌发。在相同处理条件下,滇重楼[Paris polyphylla Sm. var. yunnanensis(Franch.)Hand.-Mazz]比华重楼[Paris polyphylla Sm. var. chinese(Franch.)H. Hara]具有更好的发芽效率和稳定性。针对适宜重楼生长的较高海拔生境下种子发芽相关研究缺乏,需要针对较高海拔重楼繁育技术进行研究。     

不同处理方式对华北落叶松抚育剩余物分解的影响

[目的]为提高华北落叶松抚育剩余物的利用率,探索不同处理方式对抚育剩余物分解速率的影响,加快抚育剩余物分解速率,恢复华北落叶松人工林地力,使其保持长期较高的生产力.[方法]以华北落叶松人工林抚育剩余物为研究对象,采用粉碎、尿素、EM菌和木醋液4种处理方法,进行4因素5水平的正交试验设计.通过对剩余物进行25种处理,研究...     

芜湖市5所公园地被植物应用状况分析

为进一步明确地被植物在城市公园中的景观应用,采用实地调研的方法,记录并分析安徽省芜湖市内的赭山公园、镜湖公园、九莲塘公园、滨江公园(一期)和雕塑公园的地被植物应用状况.结果显示,5所公园常用地被植物共有94种,其中草本41种、木本53种,麦冬等7种草本及洒金桃叶珊瑚等32种木本地被植物应用最多;各公园的滨水区域地被植物...