Endemic infections of bovine viral diarrhea virus genotypes 1b and 2a isolated from cattle in Japan between 2014 and 2020

Bovine viral diarrhea virus (BVDV) is a causative agent of bovine viral diarrhea. In Japan, a previous study reported that subgenotype 1b viruses were predominant until 2014. Because there is little information regarding the recent epidemiological status of BVDV circulating in Japan, we performed genetic characterization of 909 BVDV isolates obtained between 2014 and 2020. We found that 657 and 252 isolates were classified as BVDV-1 and BVDV-2, respectively, and that they were further subdivided into 1a (35 isolates, 3.9%), 1b (588, 64.7%), 1c (34, 3.7%), and 2a (252, 27.7%). Phylogenetic analysis using entire E2 coding sequence revealed that a major domestic cluster in Japan among BVDV-1b and 2a viruses were unchanged from a previous study conducted from 2006 to 2014. These results provide updated information concerning the epidemic strain of BVDV in Japan, which would be helpful for appropriate vaccine selection.     

Feeding habits of Baird's beaked whale Berardius bairdii, in the western North Pacific and Sea of Okhotsk off Japan

ABSTRACT:   We examined the stomach contents of 26 Baird's beaked whales caught off the coast of Japan by small-type coastal whalers. The main prey for these whales was rat-tails and hakes in the western North Pacific. Pollock and squids were also important food in the whales collected from the southern Sea of Okhotsk. The prey species found in the stomachs of the whales were almost identical to those caught in bottom-trawl nets at depths greater than about 1000 m in the western North Pacific, which suggests that the Baird's beaked whale forages for prey at depths of about 1000 m or more. Baird's beaked whales in the western North Pacific migrate to waters of 1000–3000 m in depth, where demersal fish are abundant. This implies that Baird's beaked whales migrate to waters where demersal fish, especially rat-tails and hakes, are abundant. Although there is limited information on the feeding habits of ziphiid whales, they are generally thought to prefer squid. The present data suggest that demersal fish are also important prey for ziphiid whales.     

Establishment of a mouse- and egg-adapted strain for the evaluation of vaccine potency against H3N2 variant influenza virus in mice

Sporadic spreads of swine-origin influenza H3N2 variant (H3N2v) viruses were reported in humans, resulting in 437 human infections between 2011 and 2021 in the USA. Thus, an effective vaccine is needed to better control a potential pandemic for these antigenically distinct viruses from seasonal influenza. In this study, a candidate vaccine strain with efficient growth capacity in chicken embryos was established through serial blind passaging of A/Indiana/08/2011 (H3N2)v in mice and chicken embryos. Seven amino acid substitutions (M21I in PA; A138T, N165K, and V226A in HA; S312L in NP; T167I in M1; G62A in NS1 proteins) were found in the passaged viruses without a major change in the antigenicity. This mouse- and egg-adapted virus was used as a vaccine and challenge strain in mice to evaluate the efficacy of the H3N2v vaccine in different doses. Antibodies with high neutralizing titers were induced in mice immunized with 100 µg of inactivated whole-virus particles, and those mice were significantly protected from the challenge of homologous strain. The findings indicated that the established strain in the study was useful for vaccine study in mouse models.     

Risk profile of low pathogenicity avian influenza virus infections in farms in southern Vietnam

The impact of low pathogenicity avian influenza (LPAI) has been confirmed mainly in farms. Unlike apparent losses caused by the high pathogenicity avian influenza (HPAI), the LPAI impact has been hardly evaluated due to underestimating its spread and damage. In 2019, a questionnaire study was conducted in southern Vietnam to identify the specific risk factors of LPAI virus (LPAIV) circulation and to find associations between husbandry activities and LPAI prevalence. A multilevel regression analysis indicated that keeping Muscovy ducks during farming contributed to LPAIV positivity [Odds ratio=208.2 (95% confidence interval: 13.4–1.1 × 10⁴)]. In cluster analysis, farmers willing to report avian influenza (AI) events and who agreed with the local AI control policy had a slightly lower risk for LPAIV infection although there was no significance in the correlation between farmer characteristics and LPAI occurrence. These findings indicated that keeping Muscovy ducks without appropriate countermeasures might increase the risk of LPAIV infection. Furthermore, specific control measures at the local level are effective for LPAIV circulation, and the improvement of knowledge about biosecurity and attitude contributes to reducing LPAI damage.     

Estimation of seasonal spawning ground locations and ambient sea surface temperatures for eggs and larvae of Pacific saury (Cololabis saira) in the western North Pacific

Since there have been practically no surveys of the eggs of Pacific saury (Cololabis saira) in the western North Pacific (WNP), its spawning ground (SG) distribution has been poorly resolved, based mainly on the larval distribution. This means of estimating SG distribution is imprecise because saury eggs drift for more than a week before they hatch, in a region with intense western boundary currents and their extensions. To improve our understanding of the immature saury, a large number of larvae (body length <25 mm) collected in the WNP during 1993–96 were numerically backtracked to take into account the advection by geostrophic and wind‐forced Ekman currents, and the SG locations and ambient sea surface temperatures (SSTs) for the eggs and larvae on the backtracking trajectories were estimated. The resulting seasonal distributions of SGs indicated that both the locations and the intensities of spawning change from season to season. Moreover, the ambient SSTs for eggs just after fertilization ranged from a high of around 21.5°C in early autumn (September to October) to a low of around 15.0°C in late spring (May to June) with an intermediate of around 20.0°C in winter (January to February). The ambient SSTs showed seasonally different gradients while the individuals developed from eggs to early larvae: the SSTs decreased throughout the autumn (September to December), stayed rather constant in winter (January to February), and increased throughout the spring (March to June). The ambient SSTs for the early larvae were at around 19.0°C in autumn and winter (September to February) and around 16.5°C throughout the spring (March to June).     

新疆石河子棉区高密条件下冠层结构和光分布特征

对新疆石河子5个棉花主栽品种全生育期的干物质积累、生长参数、产量和盛铃期的冠层结构、光渗透能力和光分布等进行了测量分析。结果表明,作物生长速率、铃生长率以及子棉产量等均与总受光量呈正相关关系,净同化率则与单位叶面积受光量呈正相关关系,说明受光量是决定干物质生产和产量的重要因素;单位叶面积受光量即光的渗透能力与单位土地面积总受光量呈正相关关系、而与LAI呈负相关关系,说明冠层结构也是影响冠层受光量的主要因素;总受光量与LAI呈显著的正相关关系,说明LAI最大值并未达到最适宜水平。新疆地区棉花高产棉田的主要特征可以总结为叶数少、叶片大、冠层结构为椭圆形。由于叶面积指数尚未达到最适宜水平,增加种植密度可能是提高产量的一个途径。