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1.
Takafumi SUZUKI Naohito NISHII Satoshi TAKASHIMA Tatsuya MATSUBARA Atsushi IWASAWA Hirofumi TAKEUCHI Kohei TAHARA Tatsuyuki HACHISU Hitoshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1379-1383
Polyclonal immunoglobulin (Ig) G autoantibodies against insulin have been
identified in sera of healthy cats. We purified and fractionated insulin-binding IgGs from
cat sera by affinity chromatography and analyzed affinity of insulin-binding IgGs for
insulin and their epitopes. Following the passing of fraction A, which did not bind to
insulin, insulin-binding IgGs were eluted into two fractions, B and C, by affinity
chromatography using a column fixed with bovine insulin. Dissociation constant (KD) values
between insulin-binding IgGs and insulin, determined by surface plasmon resonance analysis
(Biacore™system), were 1.64e−4 M for fraction B (low affinity IgGs) and
2e−5 M for fraction C (high affinity IgGs). Epitope analysis was conducted
using 16 peptide fragments synthesized in concord with the amino acid sequence of feline
insulin by an enzyme-linked immunosorbent assay. Fractions B and C showed higher
absorbance (affinity) of the peptide fragment of 10 amino acid residues at the
carboxyl-terminal of the B chain (peptide No. 19), followed by peptide fragments of 6 to
15 amino acid residues of the B chain (peptide No. 8). Fraction C showed a higher
absorbance to 7 to 16 amino acid residues of the B chain (peptide No. 5) compared with the
absorbance of fraction B. Polyclonal insulin-binding IgGs may form a macromolecule complex
with insulin through the multiple affinity sites of IgG molecules. Feline insulin-binding
IgGs are multifocal and may be composed of multiple IgG components and insulin. 相似文献
2.
Yasuo Tomida Toshiya Suzuki Tsutomu Yamada Ryuji Asami Hirofumi Yaegashi Yasufumi Iryu Tsuguo Otake 《Fisheries Science》2014,80(2):273-280
We examined differences in otolith oxygen (δ18Ootolith) and carbon (δ13Cotolith) stable isotope ratios between hatchery and wild pink salmon fry. The δ18Ootolith values of hatchery and wild fry were ?7.7 ± 0.2 ‰ and ?8.3 ± 0.3 ‰ (1σ), respectively. This difference reflected differences in temperature conditions experienced by each fry. The δ13Cotolith values of hatchery and wild fry were ?19.2 ± 0.3 ‰ and ?11.1 ± 1.8 ‰ (1σ), respectively. The lower δ13Cotolith values of hatchery fry were probably related to their intake of artificial diets. Discriminant analysis of δ18Ootolith and δ13Cotolith values demonstrated a highly significant difference between hatchery and wild fry with 95.8 % classification accuracy. Therefore, analysis of δ18Ootolith and δ13Cotolith precipitated in the fry stage may be useful for discriminating the origin of returning adult pink salmon. The discrimination method for returning adult fish would provide important information for evaluating the effect of hatchery release and their impact on the wild population. 相似文献
3.
Analysis of the Abnormal Segregation of Pathogenicity in Magnaporthe grisea by Using a Genetic Cross of Oryza and Eleusine Isolates 总被引:2,自引:0,他引:2
A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showed that the segregation of pathogenicity in this genetic cross was abnormal, i.e., most of the progeny isolates were nonpathogenic on both host plants. However, no abnormal segregation was observed when middle repetitive sequence MGR586 and 31 single-copy RFLP markers from all of the chromosomes were genetically analyzed. At the same time, comparison of the chromosomal organization among two pairs of parental isolates did not find any genomic abnormity. These results suggested that the "abnormal" inheritance of pathogenicity in this cross was most likely due to the reassortment of numerous host species specificity genes but not the biased segregation of the host species specificity genes. The host species specificities in M. grisea were likely to be multigenically controlled, at least in the genetic cross involving rice pathogen and the grasses pathogen other than rice. 相似文献
4.
Hirofumi Ido Hirofumi Nagao Hideo Kato Atsushi Miyatake Yasushi Hiramatsu 《Journal of Wood Science》2010,56(5):422-428
Tests of compression perpendicular to the grain were carried out on laminated veneer lumber (LVL) and timber. The species
tested were sugi, radiata pine, karamatsu, akamatsu, and dahurian larch; two sets of sugi specimens were tested, with the
sugi LVL products being manufactured in different plants. The strength properties of the materials for different loading directions
were compared for LVL and timber. At 5% compressive strain in the same materials, the average stress in the tangential direction
of timber was larger than that in the radial direction for all species except for radiata pine, and the average stress in
the edge-wise direction of LVL was larger than that in the flat-wise direction for all species except for radiata pine. When
the stress at 5% strain was compared in the same direction, the average stress of LVL in the edge-wise direction was larger
than that in timber in the tangential direction for all species, but there were no great differences between the average stress
of LVL in the fl at-wise direction and that of timber in the radial direction for all species except for radiation pine. There
was a close relationship between density and stress at 5% strain in LVL, especially in the edge-wise direction. For all results,
radiata pine did not follow the trend of the other species; The large annual ring width of radiata pine was considered to
have affected the results. 相似文献
5.
6.
Yoshio MINAMI Seiko YAMANO Minako KAWAI Atsushi HIRAGA Hirofumi MIYATA 《Journal of Equine Science》2009,20(3):33-40
To find a new parameter indicating muscle fitness in Thoroughbred horses, we examined
time-dependent recovery of glycogen content and sarcoplasmic reticulum (SR)
Ca2+-ATPase activity of skeletal muscle after intensive treadmill running.
Two repeated 50-sec running sessions (13 m/sec) were performed on a flat treadmill
(approximately 90%VO2max). Muscle samples of the middle gluteal muscle were
taken before exercise (pre) and 1 min, 20 min, 60 min, and 24 hr after exercise. Muscle
fiber type composition was determined in the pre muscle samples by immunohistochemical
staining with monoclonal antibody to myosin heavy chain. SR Ca2+-ATPase
activity of the muscle and glycogen content of each muscle fiber type were determined with
biochemical analysis and quantitative histochemical staining, respectively. As compared to
the pre value, the glycogen content of each muscle fiber type was reduced by 15–27% at 1
min, 20 min, and 60 min after the exercise and recovered to the pre value at 24 hr after
exercise test. These results indicate that 24 hr is enough time to recover glycogen
content after short-term intensive exercise. The mean value of the SR
Ca2+-ATPase activity showed a slight decrease (not significant) immediately
after exercise, and complete recovery at 60 min after exercise. There were no significant
relationship between the changes in glycogen content of each muscle fiber type and SR
Ca2+-ATPase. Although further studies are needed, SR Ca2+-ATPase
is not a useful parameter to detect muscle fitness, at least in Thoroughbred horses. 相似文献
7.
Effects of apple pomace‐mixed silage on growth performance and meat quality in finishing pigs 下载免费PDF全文
Jiachen Fang Yang Cao Masatoshi Matsuzaki Hiroyuki Suzuki Hirofumi Kimura 《Animal Science Journal》2016,87(12):1516-1521
We measured the growth performance and meat quality of 10 crossbred (Yorkshire × Duroc × Landrace) neutered male pigs to evaluate the effects of apple pomace‐mixed silage (APMS). The pigs were divided into two groups and were respectively fed the control feed and the AMPS ad libitum during the experiment. No difference was found in the finished body weight, average daily gain, carcass weight, back fat thickness or dressing ratio between the control and the AMPS treatments, but average dairy feed intake (dry matter) was significantly lower and feed efficiency was significantly higher using the APMS treatment (P < 0.05). With regard to meat quality, the APMS increased the moisture content but decreased the water holding capacity (P < 0.05) compared with the control treatment. Furthermore, the APMS affected the fatty acid composition of the back fat by increasing linoleic acid (C18:2n6), linolenic acid (C18:3) and arachidic acid (C20:0) levels, while decreasing palmitic acid (C16:0), palmitoleic acid (C16:1) and heptadecenoic acid (C17:1) levels, compared with the control treatment. These results indicate that feeding fermented apple pomace to finishing pigs increases the feed efficiency and affects the meat quality and fatty acid composition of back fat. 相似文献
8.
Seiko YAMANO Minako KAWAI Yoshio MINAMI Atsushi HIRAGA Hirofumi MIYATA 《Journal of Equine Science》2010,21(4):59-65
We evaluated differences in muscle fiber recruitment patterns between continuous and
interval training to develop an optimal training program for Thoroughbred horses. Five
well trained female thoroughbred horses (3–4 years old) were used. The horses performed
two different exercises on a 10% inclined treadmill: 90%VO2 max for 4 min
(continuous) and 90% VO2 max for 2 min × 2 times with 10-min interval
(interval). Muscle samples were obtained from the middle gluteal muscle before and
immediately after the exercises. Four muscle fiber types (type I, IIA, IIA/X, and IIX)
were immunohistochemically identified, and the optical density of periodic acid Schiff
staining (OD-PAS) in each fiber type and glycogen content of the muscle sample were
determined by quantitative histochemical and biochemical procedures, respectively. No
significant differences were found in the OD-PASs and glycogen contents between the
continuous and interval exercises, but the decreases in OD-PAS of fast-twitch muscle
fibers were obvious after interval as compared to continuous exercise. Interval exercise
may be a more effective training stimulus for the glycolytic capacity of fast-twitch
muscle fiber. The data about muscle fiber recruitment can provide significant insights
into the optimal training program not only for thoroughbred horses, but also for human
athletes. 相似文献
9.
10.