Effect of biofloc technology on growth of speckled shrimp,Metapenaeus monoceros (Fabricus) in different feeding regimes

The effects of biofloc technology (BFT) were tested on growth performance, water quality, haemolymph parameters and hepatopancreas histology with and without BFT for the speckled shrimp. In this study, eight different experimental groups were formed (four BFT and four control groups) to measure the effects of different feeding rates on compensatory growth of speckled shrimp. BFT treatments were performed with zero water exchange and planned as BFT1: gradually feed decreasing group 4, 2 and 1% bw, BFT2: 4% bw, BFT3: 2‐days feed with 4% bw, 1‐day fasted, and BFT4: 1‐day feed with 4% bw, 1‐day fasted. In control treatment, the same trial groups were constituted for comparing with BFT at a 50% daily water exchange: C1 (gradually feed decreasing group: 4%, 2% and 1% bw), C2 (4% bw), C3 (2‐days feed with 4% bw, 1‐day fasted), C4 (1‐day feed with 4% bw, 1‐day fasted). There were significant differences between BFT and C groups in terms of some water quality (p < 0.05). In general, BFT had an effect on haemocyte profile. At the histological examination, there were no pathological findings in both BFT and control groups. The growth parameters of speckled shrimps in BFT groups, as well as the whole body crude protein ratios and crude ash contents, were higher than the control group (p < 0.05). The results confirmed that biofloc utilization increased with decreasing feeding.     

Wheel traffic impact on soil conditions as influenced by tillage system in Central Anatolia

The increased limiting effects of soil compaction on Central Anatolian soils in the recent years demonstrate the need for a detailed analysis of tillage system impacts. This study was undertaken to ascertain the effects of seven different tillage systems and subsequent wheel traffic on the physical and mechanical properties of typical Central Anatolian medium textured clay loam soil (Cambisol), south of Ankara, Turkey. Both tillage and field traffic influenced soil bulk density, porosity, air voids and strength significantly except the insignificant effect of traffic on moisture content. Traffic affected the soil properties mostly down to 20 cm. However, no excessive compaction was detected in 0–20 cm soil depth. The increases of bulk density following wheel traffic varied between 10–20% at 0–5 cm and 6–12% at 10–15 cm depth. In additions, traffic increased the penetration resistance by 30–74% at 0–10 cm and 7–33% at 10–20 cm. Less wheel traffic-induced effects were found on chisel tilled plots, compared to ploughed plots. Soil stress during wheel passage was highly correlated with soil strength. Also, both tillage and traffic-induced differences were observed in mean soil aggregate sizes, especially for mouldboard ploughed plots. The obtained data imply that chisel+cultivator-tooth harrow combination provides more desirable soil conditions for resisting further soil compaction.     

Physiological stress and innate immune response in gilthead sea bream (<Emphasis Type="Italic">Sparus aurata)</Emphasis> and sea bass (<Emphasis Type="Italic">Dicentrarchus labrax</Emphasis>) exposed to combination of trimethoprim and sulfamethoxazole (TMP-SMX)

The purpose of this study was to determine whether gilthead sea bream and sea bass treated with combination of trimethoprim and sulfamethoxazole (TMP-SMX) differed in terms of physiological and innate immune biomarkers. Fish were exposed to TMP-SMX at 40 ppm concentration for 1 h as a prophylactic usage. Plasma cortisol, glucose, electrolytes (Ca, P, Na, K, Cl, and Mg) as well as plasma lysozyme activity, C-reactive protein (CRP), and ceruloplasmin (Cp) were measured soon after treatment and following 24 and 48 h in normal sea water for recovery. Treatment with TMP-SMX in both gilthead sea bream and sea bass led to an increase in plasma cortisol and glucose. Fluctuations in some electrolytes were found after treatment and during recovery period, however, the ratios of monovalent ions in treated sea bream were similar to control. Hematocrit values as well as plasma lysozyme activity in gilthead sea bream and sea bass were not affected by the treatment. CRP in gilthead sea bream and Cp in sea bass responded to the treatment with decreased levels. Both gilthead sea bream and sea bass displayed a rapid physiological stress response and sensitivity to TMP-SMX exposure, which requires more than 48-h period for regaining homeostasis.     

Evaluation of the secondary stress response in healthy Nile tilapia (Oreochromis niloticus L.) after treatment with a mixture of formalin, malachite green and methylene blue

To determine the effects of exposure to a mixture of formalin, malachite green and methylene blue (FMC) on the secondary stress indices, changes in glucose, phosphorus, calcium, magnesium, sodium, potassium and haematocrit were monitored in healthy Nile tilapia, Oreochromis niloticus L. Fish were exposed separately to varying concentrations of a mixture of formalin, malachite green and methylene blue (0.1, 0.5 and 1 p.p.m.) for 1, 10 and 60 min. In general, treatment of fish with FMC elicited marked elevations of plasma glucose. Plasma phosphorus levels dropped after FMC treatment. In fish exposed to FMC, calcium levels in general were lower than those of the controls. Magnesium levels were not influenced by FMC treatment. Plasma sodium and potassium levels showed an unclear pattern for differing FMC concentrations and exposure times. Haematocrit values were affected by FMC treatment.     

Vulnerability of Bavarian silty loam soil to compaction under heavy wheel traffic: impacts of tillage method and soil water content

Soil compaction caused by traffic of heavy vehicles and machinery has become a problem of world-wide concern. The aims of this study were to evaluate and compare the changes in bulk density, soil strength, porosity, saturated hydraulic conductivity and air permeability during sugar beet (Beta vulgaris L.) harvesting on a typical Bavarian soil (Regosol) as well as to assess the most appropriate variable factors that fit with the effective controlling of subsequent compaction. The field experiments, measurements and laboratory testing were carried out in Freising, Germany. Two tillage systems (conventional plough tillage and reduced chisel tillage) were used in the experiments. The soil water contents were adjusted to 0.17 g g⁻¹ (w₁), 0.27 g g⁻¹ (w₂) and 0.35 g g⁻¹ (w₃).Taking the increase in bulk density, the decrease in air permeability and reduction of wide coarse pore size porosity (−6 kPa) into account, it seems that CT (ploughing to a depth of 0.25 m followed by two passes of rotary harrow to a depth 0.05 m) of plots were compacted to a depth of at least 0.25 m and at most 0.40 m in high soil water (w₃) conditions. The trends were similar for “CT w₁” (low soil water content) plots. However, it seems that “CT w₁” plots were less affected than “CT w₃” plots with regard to bulk density increases under partial load. In contrast, diminishments of wide coarse pores (−6 kPa) and narrow (tight) coarse pores (−30 kPa) were significantly higher in “CT w₁” plots down to 0.4 m. Among CT plots, the best physical properties were obtained at medium soil water (w₂) content. No significant increase in bulk density and no significant decrease in coarse pore size porosity and total porosity below 0.2 m were observed at medium soil water content. The soil water content seemed to be the most decisive factor.It is likely that, CS (chiselling to a depth of 0.13 m followed by two passes of rotary harrow to a depth 0.05 m) plots were less affected by traffic treatments than CT plots. Considering the proportion of coarse pore size porosity (structural porosity) and total porosity, no compaction effects below 0.3 m were found. Medium soil water content (w₂) provides better soil conditions after traffic with regard to wide coarse pore size porosity (−6 kPa), air permeability (at 6 and 30 kPa water suction), total porosity and bulk density. Proportion of wide coarse pores, air permeability and bulk density seems to be suitable parameters to detect soil compaction under the conditions tested.     

Cortisol and finfish welfare

Previous reviews of stress, and the stress hormone cortisol, in fish have focussed on physiology, due to interest in impacts on aquaculture production. Here, we discuss cortisol in relation to fish welfare. Cortisol is a readily measured component of the primary (neuroendocrine) stress response and is relevant to fish welfare as it affects physiological and brain functions and modifies behaviour. However, we argue that cortisol has little value if welfare is viewed purely from a functional (or behavioural) perspective—the cortisol response itself is a natural, adaptive response and is not predictive of coping as downstream impacts on function and behaviour are dose-, time- and context-dependent and not predictable. Nevertheless, we argue that welfare should be considered in terms of mental health and feelings, and that stress in relation to welfare should be viewed as psychological, rather than physiological. We contend that cortisol can be used (with caution) as a tractable indicator of how fish perceive (and feel about) their environment, psychological stress and feelings in fish. Cortisol responses are directly triggered by the brain and fish studies do indicate cortisol responses to psychological stressors, i.e., those with no direct physicochemical action. We discuss the practicalities of using cortisol to ask the fish themselves how they feel about husbandry practices and the culture environment. Single time point measurements of cortisol are of little value in assessing the stress level of fish as studies need to account for diurnal and seasonal variations, and environmental and genetic factors. Areas in need of greater clarity for the use of cortisol as an indicator of fish feelings are the separation of (physiological) stress from (psychological) distress, the separation of chronic stress from acclimation, and the interactions between feelings, cortisol, mood and behaviour.     

Changes of Non-Specific Immune Parameters in Rainbow Trout,Oncorhynchus mykiss,After Exposure to Antimicrobial Agents Used in Aquaculture

Changes of plasma lysozyme and serum bactericidal activity, ceruloplasmin, total plasma protein, serum glucose, hematocrit and leucocrit levels in rainbow trout (Oncorhynchus mykiss) were studied after exposure to formalin and chloramine-T, which are used in aquaculture as anti-ectoparasitic and antibacterial agents. Fish were exposed to formalin at a concentration of 250 ppm for 60 min and exposed to chloramine-T for 5 ppm for 3 h. The parameters were analyzed for rainbow trout exposed to formalin and chloramine-T soon after treatments and rainbow trout exposed to freshwater after 24 h (return) following the treatments. Plasma lysozyme activity decreased after exposure to formalin; however, it was not affected by chloramine-T exposure. Serum bactericidal activity reduced after both formalin and chloramine-T exposure and did not change after returning to freshwater. Ceruloplasmin and total plasma protein were not affected by both treatments. Serum glucose levels of exposed fish elevated and remained at higher levels during recovery period when compared to control values. Hematocrit values of fish exposed to formalin increased; however, no significant change was observed in fish exposed to chloramine-T. Leucocrit levels of fish treated with both formalin and chloramine-T were higher than that of controls.