Carryover of maduramicin from feed containing cross-contamination levels into eggs of laying hens

Maduramicin is a coccidiostat authorized as feed additive in the European Union for chickens and turkeys for fattening but not for laying hens, considering the risk of residues in eggs. The unavoidable cross-contamination of non-target feed with coccidiostats is regulated by Commission Directive 2009/8/EC and resulting carry-over in food by Commission Regulation (EC) No. 124/2009. To verify the compliance of the maximum levels for maduramicin in feed (50 μg/kg) and eggs (2 μg/kg), the carry-over from feed into eggs was investigated. Diets containing 10, 30, and 50 μg of maduramicin/kg of feed were fed to laying hens. Feed, egg white, and yolk were analyzed by LC-MS/MS. Maduramicin residues were only detected in in egg yolk. Feeding the 10 μg/kg maduramicin diet resulted in maduramicin concentrations up to 2.5 μg/kg in whole eggs, already exceeding the maximum level. A carry-over rate of 8% maduramicin from feed into eggs was calculated.     

Combining agent-based and stock-flow modelling approaches in a participative analysis of the integrated land system in Reichraming, Austria

The integrated modelling of coupled socio-ecological systems in land-change science requires innovative model concepts capable of grasping the interrelations between socioeconomic and natural components. Here, we discuss the integrated socio-ecological model SERD (Simulation of Ecological Compatibility of Regional Development) that was developed for the municipality of Reichraming in Upper Austria in a participative 2-year process involving local stakeholders. SERD includes three main components: (1) an agent-based actors module that simulates decisions of farmsteads, the municipal administration and other important actors; (2) a spatially explicit (GIS based) land-use module that simulates land-use change at the level of individual parcels of land and (3) an integrated socio-ecological stock-flow module that simulates carbon and nitrogen flows through both socioeconomic and ecological system compartments. We report on outcomes of a scenario analysis that outlines possible future trajectories depending on both external (e.g. agricultural subsidies and prices) and internal (e.g. innovation, willingness to co-operate) factors. We find that both external and internal factors can affect the behaviour of the integrated system considerably. Local and regional policies are found to be able to counteract adverse global socioeconomic conditions to some extent, but not to reverse the trend altogether. We also find strong interdependencies between socioeconomic and ecological components of the system. Fully evaluating these interdependencies is, however, not possible at the local scale alone and will require explicit consideration of higher-level effects in future research.     

Über das Auftreten des Dickmaulrüsslers Otiorrhynchus ovatus L. als Schädling der Erdbeeren (Fragaria)

Zusammenfassung und Schlußbetrachtung Otiorrhynchus ovatus L. ist in Deutschland fast ausschließlich als Forstschädling beschrieben worden. Erheblicher Schaden an Erdbeeren wurde bisher namentlich aus Nordamerika bekannt. Der genannte Rüßler vergrößert die Zahl der vermutlich infolge veränderter ökologischer Verhältnisse während der letzten Jahre in Deutschland aufgetretenen Schadinsekten, soweit es die Erdbeerkultur betrifft. Die Art ist polyphag; doch kommt ihr Schaden nur in mehrjährig perennierenden Kulturanlagen infolge der ungestörten Entwicklungsmöglichkeit zur vollen Auswirkung.Im Gebiet von Strehla wurde sowohl im Jahre 1946 als auch 1949 durch die Fraßtätigkeit der Larven ca. 1/5 der gesamten Anbaufläche total vernichtet.Erhöhte Aufmerksamkeit ist für die gefährdeten Kulturen geboten. Erneutes Auftreten des Käfers nach zweijähriger Pause in dreijähriger Anbaufläche des beschriebenen Befallsgebietes würde die Annahme festigen, daß die Generationsdauer im vorliegenden Falle dreijährig ist. Es wurde vorgesehen, durch laufende Bodenuntersuchungen in den gefährdeten Kulturen die Larven im ersten Entwicklungsstadium festzustellen. Gegebenenfalls soll durch Züchtung in der Gefangenschaft zur Klärung des Entwicklungszyklus beigetragen werden.Die Bekämpfung bleibt, wenn infolge unzureichender Vorbeugungsmaßnahmen totaler Schaden eingetreten ist, im wesentlichen darauf beschränkt, durch tiefes Umpflügen der stark befallenen Kulturen, noch bevor die ersten Käfer erscheinen, einen möglichst großen Teil Larven und Puppen auf mechanische Weise zu vernichten. Zur Erkenntnis, wieweit DDT-Präparate und evtl. auch geruchlose Hexa-Mittel zur Bekämpfung brauchbar sind, bedarf es noch planmäßiger Versuche im Freiland.Zukünftige Nachforschungen, wozu sachdienliche Beobachtungen und Mitteilungen aus anderer Gegend sehr erwünscht sind, sollen Aufschluß darüber gehen, ob der Käfer weiterhin als Erdbeerschädling auftritt, oder ob das Massenvorkommen in den Jahren 1946 und 1949 als eine auf das Gebiet von Strehla beschränkte Kalamität anzusehen ist.     

Hepatocellular adaptation to extreme nutritional conditions in ide,Leuciscus idus melanotus L. (Cyprinidae). A morphofunctional analysis

The adaptive response of the omnivorous ide,Leuciscus idus melanotus, to drastic metabolic conditions was analyzed on different levels of organisation investigating a variety of parameters: Organism (condition factor, liver-somatic index), organ (liver structure), cellular and subcellular level (hepatocyte structure, glycogen and lipid storage, contents and distribution of nucleic acids, enzyme alterations).During starvation, ide were able to maintain liver integrity in a biphasic process: after an initial phase of disturbance, ide established a new structural and metabolic homeostasis. Recovery from starvation was possible only with a complete diet but not with a sucrose diet. Carbohydrate overload, as evoked by sucrose refeeding, did not result in liver or carcass fattening as known from mammals.To the best of our knowledge, the present study is the first to use enzyme histochemistry in fish nutrition research. In mammals, histochemistry is particularly helpful for understanding processes of hepatic metabolic adaptation. In fish, however, on the basis of our results, enzyme histochemical studies appear to be of limited value, as long as no further data are available on a zonal distribution of enzyme activities in teleost liver parenchyma. Instead, the histochemical detection of the distribution of hepatic storage products and RNA-positive material yielded important information on liver adaptive processes.Abbreviations H starvation - SU sucrose - AD artificial diet - AcP acid phosphatase - G6Pase glucose-6-phosphatase - G6PDH glucose-6-phosphate dehydrogenase - GLU -glucuronidase - ME malic enzyme - PHO glycogen phosphorylase - UE unspecific esterase - RER rough endoplasmic reticulum     

Farms in the black forest of Baden-Württemberg: A historical review

Today the Black Forest is an internationally renowned tourist region in Germany. For centuries the Black Forest remained as a low mountain range regarded as not suitable for settlement due to its unfavourable climatic conditions and difficult terrain. Clearing the dense forests and settlement therefore started at a historically late stage compared to other regions of Germany, about 1000 years ago. Due to the special conditions in this area, the structure of the settlement had to be quite different to those landscapes with more favourable conditions for agriculture. A single-farm settlement developed, farms situated 200 to 400 m away from each other, surrounded by meadows, agricultural land and forests and owned by the farmer’s family. This structure has remained in some areas until today; in other areas different types of settlement took place, and great changes occurred during history. A special type of agroforestry — a slash-and-burn-management of forests — also took place 150 to 200 years ago.     

Konkurrenzerscheinugen in Parasitenkomplexen als Problem der Biologischen Schädlinfbekämpfung

Ohne Zusammenfassung     

Content of polyphenolic compounds in the Nigerian stimulants Cola nitida ssp. alba, Cola nitida ssp. rubra A. Chev, and Cola acuminata Schott & Endl and their antioxidant capacity

Varieties of kola nuts (Cola nitida alba, Cola nitida rubra A. Chev, and Cola acuminata Schott & Endl), a group of popular Nigerian and West African stimulants, were analyzed for their content of secondary plant metabolites. The three varieties of the kola nuts contained appreciable levels of (+)-catechin (27-37 g/kg), caffeine (18-24 g/kg), (-)-epicatechin (20-21 g/kg), procyanidin B 1 [epicatechin-(4beta-->8)-catechin] (15-19 g/kg), and procyanidin B2 [epicatechin-(4beta-->8)-epicatechin] (7-10 g/kg). Antioxidant capacity of the extracts and purified metabolites was assessed by two HPLC-based and two colorimetric in vitro assays. Extracts of all varieties exhibited antioxidant capacity with IC 50 values in the range 1.70-2.83 and 2.74-4.08 mg/mL in the hypoxanthine/xanthine oxidase and 2-deoxyguanosine HPLC-based assays, respectively. Utilization of HPLC-based assays designed to reflect in situ generation of free radicals (e.g., HO(*)), as opposed to general assays (DPPH, FRAP) in common use which do not, indicate that, of the major secondary plant metabolites present in kola nut extracts, caffeine is potentially the more effective cancer chemopreventive metabolite in terms of its antioxidant capacity.     

Effects of the needle bladder rust (Chrysomyxa rhododendri) on Norway spruce: implications for subalpine forests

Norway spruce trees in the subalpine forests of the European Alps are frequently attacked by the needle rust Chrysomyxa rhododendri. The obligate parasite undergoes a complex life cycle with a host shift between rhododendrons (Rhododendron sp.) and Norway spruce [Picea abies (L.) Karsten] and causes a yellowing and defoliation of the current-year needles in summer. Infected trees show several anatomical, morphological and physiological modifications, including a decrease in pigment content and net photosynthesis of infected needles, lower biomass production and reduced radial and height growth. The consequences are diminished timber yield and cripple growth. Because of repeated heavy infections in recent years, forest managers report increasing difficulties in both natural regeneration and afforestation at high elevation sites, where rhododendrons occur. This review gives a summary of the present knowledge about the effects of C. rhododendri infections on Norway spruce, including so far unpublished findings and with particular attention to the phenomena of resistant trees. Implications for subalpine forests and counter strategies are discussed.