排序方式: 共有18条查询结果,搜索用时 15 毫秒
1.
Tomoaki ONODA Ryuta YAMAMOTO Kyohei SAWAMURA Harutaka MURASE Yasuo NAMBO Yoshinobu INOUE Akira MATSUI Takeshi MIYAKE Nobuhiro HIRAI 《Journal of Equine Science》2013,24(4):63-69
Percentile growth curves are often used as a clinical indicator to evaluate variations of
children’s growth status. In this study, we propose empirical percentile growth curves
using Z-scores adapted for Japanese Thoroughbred horses, with considerations of the
seasonal compensatory growth that is a typical characteristic of seasonal breeding
animals. We previously developed new growth curve equations for Japanese Thoroughbreds
adjusting for compensatory growth. Individual horses and residual effects were included as
random effects in the growth curve equation model and their variance components were
estimated. Based on the Z-scores of the estimated variance components, empirical
percentile growth curves were constructed. A total of 5,594 and 5,680 body weight and age
measurements of male and female Thoroughbreds, respectively, and 3,770 withers height and
age measurements were used in the analyses. The developed empirical percentile growth
curves using Z-scores are computationally feasible and useful for monitoring individual
growth parameters of body weight and withers height of young Thoroughbred horses,
especially during compensatory growth periods. 相似文献
2.
3.
4.
Shinichiro HIRAI Eiji YOKOYAMA Yuh SHIWA Taichiro ISHIGE Naoshi ANDO Takeshi SHIMIZU Satoshi MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(10):1399
Eighty strains of enterohemorrhagic Escherichia coli O157:H7/H- were analyzed by three single-nucleotide polymorphism (SNP) panels using whole-genome sequencing data. The partial concordance of SNP types among the different SNP panels was observed on minimum spanning trees reconstructed with SNP data. As for lineage I/II strains, some of the clade 7 strains belonged to one unique SNP type as determined by three panels, suggesting that clade 7 should be divided into at least two genotypes, namely, the unique type and the rest. In addition, clade 8 contained two unique genotypes, which was consistent with the previous prediction. Similarly, for lineage II, clade 12 should be divided into three genotype strains. In contrast, many strains of several clades belonging to lineage I were clustered into the same node on each minimum spanning tree upon testing with the three SNP panels. Previous studies reported that lineage I diverged more recently than lineages I/II and II. Such low diversity in lineage I in this study may have arisen because this lineage has not accumulated SNPs because of its relatively recent divergence. Based on the concordance observed in this study, some of the previously published O157 genotype distribution data were successfully interpreted to clarify the clade distribution, which was well supported by previous literature. 相似文献
5.
6.
Miyuki KITAOKA Shuichi HIRAI Hayato TERAYAMA Munekazu NAITO Ning QU Naoyuki HATAYAMA Hidenobu MIYASO Yoshiharu MATSUNO Masatoshi KOMIYAMA Masahiro ITOH Chisato MORI 《The Journal of reproduction and development》2013,59(5):485-490
Exposure to di-(2-ethylhexyl) phthalate (DEHP) has been reported to induce
spermatogenic disturbance through oxidant stress and affect the immune system as an
adjuvant. However, the effect of DEHP on the testicular immune microenvironment has
not yet been investigated. In the present study, we examined the testicular immune
microenvironment after exposure to doses of DEHP, previously identified as
no-observed-adverse-effect levels. Adult male mice were administered food containing
0%, 0.01% or 0.1% DEHP and then testes were analyzed. The results showed that a
slight but significant spermatogenic disturbance appeared in the 0.1% DEHP group but
not in the 0.01% DEHP group at 8 weeks. It was also demonstrated that lymphocytes and
F4/80- and MHC class II- positive cells were significantly increased with the
elevation of IL-10 and IFN-γ mRNA expressions in the testes of not only the 0.1% DEHP
group but also the 0.01% DEHP group at 8 weeks. Histochemical analyses involving
horseradish peroxidase (HRP) as a tracer showed that a little blood-borne HRP had
infiltrated into the lumen of a few seminiferous tubules beyond the
blood-testis-barrier in both the 0.1% and 0.01% DEHP groups at 8 weeks. This
indicates that a dose of DEHP that has little effects on spermatogenesis can change
the testicular immune microenvironment with functional damage of the blood-testis
barrier. 相似文献
7.
Keiko TOYAMA Takuya HIRAI Masuo SUEYOSHI Ziheng ZHOU Ahmad Massoud NIAZI Nijiho KAWAGUCHI Naoyuki FUKE Ryouji YAMAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1536
Porcine circovirus 2 (PCV2) causes porcine circovirus-associated disease, and co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) severely affects the pig breeding industry. Both viruses target the macrophages in lymphoid tissues. Various porcine pathogens enter via the nasal cavity, and the nasopharynx-associated lymphoid tissue (NALT) acts as the mucosal immune system. However, the pathological analysis has not progressed. This study aimed to histologically examine the NALT of pigs with suspected PCV2 and PRRSV infections. Six pigs were subjected to necropsy, and their NALT, tonsils, and mesenteric lymph nodes were collected. Macrophages, lymphocytic depletion, multinucleated giant cells, intracytoplasmic inclusion bodies, and neutrophil infiltration increased in the NALT. In situ hybridization revealed positive signals for PCV2 in the NALT of all pigs and PRRSV in the NALT of three pigs. PCV2-positive macrophages were mainly identified in the follicles, whereas PRRSV-positive tissues were found primarily around the crypt and directly below the epithelium. Quantitative PCR revealed 108–1010 copies of PCV2 DNA/µL and 102–104 copies of PRRSV DNA/µL in the NALT. Therefore, both PCV2 and PRRSV were detected in the NALT of pigs. In conclusion, the infection and replication of both viruses in the NALT and tonsils may suppress host immunity and promote co-infection with other pathogens. 相似文献
8.
Hiroaki SHIGEMURA Takashi MAEDA Shiko NAKAYAMA Akira OHISHI Yuki CARLE Eiko OOKUMA Yoshiki ETOH Shinichiro HIRAI Mari MATSUI Hirokazu KIMURA Tsuyoshi SEKIZUKA Makoto KURODA Nobuyuki SERA Yasuo INOSHIMA Koichi MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1345
Dissemination of extended-spectrum cephalosporin (ESC)-resistant Salmonella is a public health concern in the egg production industry. ESC-resistant Salmonella often acquires the bla gene via insertion sequences (ISs). Therefore, this study aimed to assess antimicrobial resistance in Salmonella from Japanese layer breeding chains and egg processing chains, and determine the genetic profiles of IS-like elements in ESC-resistant Salmonella. Antimicrobial susceptibility testing was performed on 224 isolates from 49 facilities involving layer breeder farms, hatcheries, pullet-rearing farms, and layer farms in breeding chains along with egg processing chains. ESC-resistant Salmonella strains were whole-genome sequenced. Among them, 40 (17.9%) were resistant to at least streptomycin, tetracycline, ampicillin, chloramphenicol, cefpodoxime, nalidixic acid, ciprofloxacin, and/or kanamycin despite lacking resistance to azithromycin and meropenem. Moreover, 15 were ESC-resistant Salmonella harboring blaCMY-2 (Salmonella enterica serovar Ohio, n=12; S. Braenderup, n=1; untypeable with O7:b:-, n=1) and blaCTX-M-14 (S. Cerro, n=1). IncA/C2 plasmids containing ISEcp1, IS26, and multiple antimicrobial resistance genes (including blaCMY-2) were identified in S. Ohio isolates from pullet-rearing and layer farms belonging to the same company. Chromosomal integration of partial or whole IncA/C2 plasmids was seen with two S. Ohio isolates via ISEcp1 or IS26, respectively. Antimicrobial resistance genes such as blaCMY-2 might be transmitted among the upper and the lower levels of layer breeding chains via the replicon type IncA/C2 plasmids containing ISEcp1 and IS26. 相似文献
9.
Kenji ONO Kouji MIKI Masahiro AMARI Keizo HIRAI 《Soil Science and Plant Nutrition》2008,54(2):188-196
10.
J. HIRAI H. MIYAZAKI T. TANEIKE 《Journal of veterinary pharmacology and therapeutics》1992,15(3):231-239
The correlation between pharmacokinetics and dynamics of furosemide was investigated in anaesthetized dogs. After intravenous administration (i.v.) of furosemide (5 mg/kg), the plasma concentration declined rapidly with bioexponential decay. The half-life (t1/2 beta) of the late phase of elimination was 0.931 +/- 0.187 h and the apparent volume of distribution at steady state was 0.25 +/- 0.043 l/Kg. The total clearance (Cltot) was 0.435 +/- 0.031 l/h/kg, in which the renal clearance was 0.260 +/- 0.020 (about 60% of Cltot). The change in rate of urinary excretion of furosemide was similar to the plasma concentration decay curve. The diuretic effect of furosemide was accompanied by an extreme increase in the excretion rate of sodium and chloride, but not potassium. The relationships between the diuretic response and the plasma concentration or the urinary excretion rate of furosemide was depicted by sigmoidal dose-response curves in both cases. The half-maximum effect was obtained at 1.5 micrograms/ml of plasma concentration or at 80 micrograms/min of excretion rate of furosemide. 相似文献