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Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
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Study on the glass transition for several processed fish muscles and its protein fractions using differential scanning calorimetry 总被引:1,自引:0,他引:1
ABSTRACT: The glass transition behavior of processed fish muscles (bonito, tuna, mackerel, sea bream, cod) and its muscle protein fractions (sarcoplasmic and myofibrillar proteins) were studied using differential scanning calorimetry. Each dried processed fish muscle and the extracted protein fractions showed clear glass transition phenomenon. The T g values of muscles and myofibrillar proteins from red muscle fishes tended to be lower than those from white muscle fishes though there was no difference on T g of sarcoplasmic proteins. The T g value of whole muscle was considerably lower than that of extracted protein fractions because of the plasticizing effects of low molecular weight materials contained in the muscle. 相似文献
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Hiroyuki UGA Yuki O. KOBAYASHI Kyoji HAGIWARA Yohachiro HONDA Toshihiro OMURA Takahide SASAYA 《Journal of General Plant Pathology》2002,68(4):378-381
The causative virus (isolate No. 4) of gentian (Gentiana spp.) mosaic, which had been identified previously as Clover yellow vein virus (C1YVV) on the basis of host range and serological reactions, was re-identified as Bean yellow mosaic virus (BYMV) on the basis of the nucleotide sequences of the gene for the coat protein (CP) and the 3′-noncoding region, as well
as the predicted amino acid sequence of CP.
Received 16 April 2002/ Accepted in revised form 19 June 2002 相似文献
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Mavit ASSAVAAREE Atsushi HAGIWARA Takayuki KOGANE Misao ARIMOTO 《Fisheries Science》2003,69(3):520-528
ABSTRACT: Tropical minute rotifer strains (SS-type) induce mixis at 30–35°C but sexual reproduction and resting egg formation do not proceed well due to rapid environmental change. The present study examined the effect of temperature regulation on rotifer Brachionus rotundiformis (Langkawi strain, SS-type) resting egg formation in small (500 mL in culture volume)- and large-scale (500 L in culture volume) experiments. Rotifers were cultured at 30°C in 15–17 p.p.t. seawater with an initial density of 1 individual (ind.)/mL. After 4 days, when cultures were in exponential growth stage with active mixis induction, the culture temperature of the experimental rotifers was changed to 25°C. Control rotifers were cultured at 30°C throughout the experiment. Fresh or frozen Nannochloropsis oculata and condensed freshwater Chlorella vulgaris were used as the rotifer diets in the small- and large-scale experiments, respectively. Significantly higher resting egg production was observed with the experimental rotifers (30→ 25°C) versus the control rotifers. In the large-scale trial, experimental rotifers produced 2.6 × 106 resting eggs during a 9-day experiment, which was 1.6-fold more than the control rotifers. Moreover, the efficiency of resting egg formation was found to increase by a factor of 1.8. The present study indicates that decreasing culture temperature from 30 to 25°C after active mixis increased resting egg formation in B. rotundiformis (SS-type). 相似文献
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Akira KUROSAWA Shuhei IKEDA Tomoko HAGIWARA Seizi SUKEMORI Yoshio KURIHARA 《Animal Science Journal》2007,78(1):47-54
The present experiment aimed to obtain basic knowledge of the role of the cecum in the digestion of protein, fiber and energy, and in the retention of digesta in rats. Twelve healthy male Sprague–Dawley strain rats were used. The experiment lasted for 8 weeks following preliminary feeding. Crude protein [CP], acid detergent fiber [ADF], and energy digestibilities were measured in weeks 1, 4, and 7 of the experiment, while the digesta retention time was measured in weeks 2, 5, and 8. At the end of the test, the colon capacity was measured after killing the rats with diethyl ether. The effects of the cecectomy were not recognized in bodyweight gain, even while the feed intake was high and the feed efficiency was low due to the cecectomy. However these effects were not recognized in the latter half of the test periods. The mean retention time of digesta for the cecectomized rats was about 2 h shorter than that for the control group of rats, while the retention time of digesta in week 8 increased compared to weeks 2 and 5. The digestibility of each substance was significantly lower following the cecectomy. The colon became swollen because of the cecectomy and this swollen colon made up for the capacity of the dissected cecum. It had been considered that the cecum does not play a role in the digestion of nutrition for growth, however, the present results showed two types of effects of cecectomy: one that disappears in accordance with time after surgery (i.e. feed intake and feed efficiency), and another that continues for a longer period (i.e. digestibility, retention time of digesta). 相似文献
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Yuichi YAMAOKA Yukiyoshi FUJIWARA Makoto KAKISHIMA Keizo KATSUYA Kengo YAMADA Hiroshi HAGIWARA 《Journal of General Plant Pathology》2002,68(1):52-56
A total of 45 single uredinial isolates of Phakopsora pachyrhizi were collected from rust-infected soybean and wild host plants (Pueraria lobata and G. soja ) at different localities in central and southwestern Japan. Eighteen pathogenic races were identified using a set of differential
varieties composed of nine cultivars of soybean and two accession lines of G. soja. Nine and 11 races were found on soybean and wild host plants, respectively. Two races were common to soybean and wild host
plants.
Received 27 April 2001/ Accepted in revised form 22 August 2001 相似文献
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Manakorn SUKMAK Minoru OKAMOTO Tatsuya ANDO Katsuro HAGIWARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1526
Borna disease virus (BoDV) is a neurotropic virus that causes several infections in humans and neurological diseases in a wide range of animals worldwide. BoDV-1 has been molecularly and serologically detected in many domestic and wild animals in Japan; however, the genetic diversity of this virus and the origin of its infection are not fully understood. In this study, we investigated BoDV-1 infection and genetic diversity in samples collected from animals in Hokkaido between 2006 and 2020. The analysis was performed by focusing on the P region of BoDV-1 for virus detection. The presence of BoDV-1 RNA was observed in samples of brain tissue and various organs derived from persistently infected cattle. Moreover, after inoculation, BoDV-positive brains were isolated from neonatal rats. The gene sequences of the P region of BoDV obtained from the rat brain were in the same cluster as the P region of the virus isolated from the original bovine. Thus, genetic variation in BoDV-1 was extremely low. The phylogenetic analysis revealed that BoDV-1 isolates obtained in this study were part of the same cluster, which suggested that BoDV-1 of the same cluster was widespread among animals in Hokkaido. 相似文献
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