Soil changes after traffic with a tracked and a wheeled forest machine: a case study on a silt loam in Sweden

Alterations to some soil physical parameters were evaluatedafter passes by a tracked forest machine and a wheeled one ofequal mass (about 20 000 kg). Measurements were made after arange of machine passes, with a maximum of eight. The measuredsoil physical parameters were dry bulk density, penetrationresistance, intrinsic air permeability, saturated hydraulicconductivity, porosity and pore-size distribution. Rut depthwas also measured. Although the wheeled machine caused deeper ruts than the trackedone, alterations caused by the two machines to the measuredsoil parameters were similar, except in the uppermost 5–10cm. The wheeled machine caused a decrease in bulk density at5 cm depth, whereas the tracked machine caused an increase,despite its lower ground pressure.     

Effect of chlorfenprop-methyl, flamprop-isopropyl and benzoylprop-ethyl on 14C2 fixation in Avena fatua L., Triticum aestivum L. and Hordeum vulgare L.

The effect of chlorfenprop-methyl, flampropisopropyl and benzoylprop-ethyl on ¹⁴CO₂ fixation was followed in wild oat (Avena fatua L.), barley (Hordeum vulgare L., cv. Ametyst), and wheat (Triticum aestivum L., cv. Mironovská). Experimental plants were exposed to a ¹⁴CO₂-enriched atmosphere in a special apparatus 2 h, 1, 3, and 9 days after the herbicide treatment. Chlorfenprop-methyl already inhibited ¹⁴CO₂ fixation in wild oat plants 2 h after the treatment. ¹⁴C-metabolite transport to the roots was strongly decreased. Both ¹⁴CO₂ fixation and ¹⁴C-metabolite level in the roots were significantly depressed in A. fatua when compared with untreated plants at the last sampling time. ¹⁴C incorporation into starch was inhibited from the first day after treatment, and on day 9 was lowered more than ten fold in treated plants. Flamprop-isopropyl inhibited ¹⁴CO₂ fixation in wild oat plants from day 3 after treatment, but benzoylprop-ethyl not until day 9. Both herbicides also decreased ¹⁴C incorporation into starch in A. fatua. Chlorfenprop-methyl also slightly decreased ¹⁴CO₂ fixation in barley on day 9. However, assimilate transport into the roots and ¹⁴C incorporation into starch were not affected. Flamprop-isopropyl inhibited ¹⁴CO₂ fixation in barley plants only on the first day after treatment, and assimilate transport was also reduced. By contrast, no differences from untreated plants were found at the end of the experiment. Benzoylprop-ethyl did not decrease either ¹⁴CO2 fixation or assimilate transport to the roots in wheat, but it inhibited starch synthesis. Atrazine depressed ¹⁴CO₂ fixation in wild oat plants by 91%, in wheat plants by 99% compared with untreated plants. Assimilate transport into the roots was also strongly inhibited. In contrast to atrazine, the effect of chlorfenprop-methyl, flamprop-isopropyl, and benzoylprop-ethyl on CO₂ fixation seems to be secondary.     

CONTRAST HARMONIC IMAGING OF THE NORMAL CANINE SPLEEN

The purpose of this study was to assess the perfusion pattern and perfusion dynamics in the normal canine spleen using contrast harmonic imaging. Twenty-five dogs without clinical or ultrasonographic evidence of splenic disease were studied. Twenty-three dogs were scanned with only manual restraint; two dogs were sedated with buprenorphin. All dogs received an intravenous bolus of a microbubble contrast medium (SonoVue). The perfusion pattern during the blood pool phase represented a skewed bell-shaped curve. A tissue-specific late phase, similar to humans, was not observed. Time/intensity curves were generated for a selected region. Mean average-derived peak intensity (PI) was 6.6dB, mean time to peak intensity calculated from the initial rise (TTP) was 25.6 s and mean area under the curve (AUC) was 523.6 dBs. If dogs were divided into two body weight groups (< or =15 and >15 kg body weight), average derived peak intensity area, time to peak intensity, and area under the curve were lower for the smaller dogs than for the larger animals. However, differences were not statistically significant (P = 0.2, 0.05, and 0.08, respectively). No significant association was found between hematocrit, hemoglobin concentration, red blood cell count, blood pressure, heart rate, age, gender, and the perfusion variables. In conclusion, these baseline data may prove useful in the evaluation of dogs with diffuse or focal splenic disease.     

遗传工程病毒与细菌在家禽疫病防制及诊断中的独特作用

1引言 目前有许多细菌和病毒疫苗已在家禽生产中应用.其中有些苗能产生有效的免疫,不过仍有一些因素限制了它们的使用,如生产成本高、接种困难、保护力不足以及对这些病原微生物和疾病相关知识的欠缺等,甚至有些疾病到现在都还没有研制出疫苗或疫苗效果很差.此外,还有一些用来诊断微生物疾病的试剂盒价格比较昂贵,或灵敏度和特异性差.如果应用遗传工程方法来研制和使用重组的细菌、病毒疫苗和诊断试剂,则可能克服上述诸多限制与不足.     

Effect of benzoylprop-ethyl on uptake, transport and metabolism of 32P in Avena fatua L. and Triticum aestivum L.

The effect of benzoylprop-ethyl on plant weight, root uptake, transport and metabolism of ³²P in wild oat and wheat plants was examined 4 h, 1,3 and 9 days after treatment. The fresh weight of wild oat plants was significantly reduced, due to herbicide action only, by day 9 after treatment. By day 3, shoot weight was decreased while root weight was significantly increased by 47%. No significant changes in plant weight were caused by benzoylprop-ethyl in wheat plants. Uptake of ³²P by treated wild oat plants decreased by 39% compared with the control, by day 9, after an initial increase; uptake of ³²P was not significantly influenced in wheat plants. By day 1 transport of ³²P to the shoots was significantly reduced in wild oat plants by 34%, whereas in wheat plants it was significantly increased by 35%. Metabolism of ³²P was already hampered in wild oat plants 4 h after treatment. The content of ³²P was reduced on the first two sampling dates in both the roots and shoots of treated plants in all fractions except in DNA in the shoots. On day 3, this decrease was apparent especially in organic, lipidic and nucleic acid fractions in the shoots; incorporation of ³²P into lipidic and RNA fractions was significantly inhibited on day 9 in both the roots and shoots of treated wild oat plants. Wheat plants responded most strongly to benzyoylprop-ethyl on day 1 after treatment, when ³²P incorporation into all fractions except DNA was hampered. Differences between treated and control wheat plants gradually levelled off on days 3 and 9 after treatment.     

The effect of chlorffenprop-methyl on 32P uptake, transport and metabolism in Avena fatua L. and Hordeum vulgare L.

The eflect of chlorfenprop-methyl on plant weight, root uptake, transport and metabolism of ³²P in wild oat and barley plants was examined 4 h. 1, 3 and 9 days after treatment. The fresh weight of treated wild oat plants became significantly less than that of the controls by day 9. There was no significant effect on the weight of barley plants during this period. Uptake of ³²P by treated wild oat planus progressively decreased during the experiment and by day 9 was significantly 70% less yhan that in the control: uptake by barley plants showed a significant 30% increase by day 9. Transport of ³²P to the shoots followed a similar pattern. In wild oat plants, transport was significantly inhibited at all sampling dales by 44–91%. In barley plants. ³²P transport to the shoots tended to be enhanced by day 9 after herbicide treatment. Metabolism of ³²P in wild oat plants was affected 4 h after treatment. The content of ³²-P in individual fractions (inorganic, organic, lipidic, and nucleic acid P) was lower in treated plants, especially in the shools. In barley plants. ³²P incorporation into the individual fractions was initially inhibited 4 h after treatment, but later corresponded to that found in the control.     

Changes in faecal bacteria associated with concentrate and forage‐only diets fed to horses in training

Reasons for performing study: Diets rich in readily fermentable carbohydrates, fed traditionally to meet the increased energy requirements of the performance horse, are associated with a number of gastrointestinal disorders that involve disturbances in the intestinal microbiota, however, these changes are poorly understood. Objectives: With the long‐term objective of improving intestinal health and to increase understanding of the relationship between diet and microbiota, the effect of feeding Standardbred horses a high‐energy forage‐only (F) diet was studied compared to a more traditional forage‐concentrate (C) diet on faecal microbiota. Methods: Diets were fed in a cross‐over design to 6 mature geldings on a scheduled training regime, both periods consisting of 29 days. DNA was extracted from faecal samples collected at 4 time points from each period, bacterial 16S rRNA genes were amplified and community composition assessed by terminal‐restriction fragment length polymorphism, cloning and sequencing. Faecal pH and cultivable lactic acid bacteria (LAB) and enterobacteria were also assessed on the final collection day of each period. Results: Diet F resulted in a microbial composition that was more stable between sampling periods and had lower counts (P<0.05) of cultivable LAB and specifically members of the Streptococcus bovis/equinus complex. Motile and swarming Lactobacillus ruminis was present in all horses on diet C and not in horses on diet F. Diet C also resulted in the increase (P<0.05) in members of Clostridiaceae cluster III and a concomitant reduction (P<0.05) in an unknown group of Bacteroidales. Conclusions and potential relevance: The greater microbial stability and reduction in LAB and members of the Streptococcus bovis/equinus complex on diet F indicate an opportunity to develop feeding strategies that support equine health and welfare. Novel changes identified in the faecal microbiota that resulted from carbohydrate inclusion merit further investigation.     

Arthroscopic Removal of Metallic Foreign Body from the Talocrural Joint Using a Magnetic Retriever in a Horse

Objective— To report use of a magnetic retriever for arthroscopic removal of a metallic foreign body from the equine talocrural joint.
Study Design— Clinical report.
Animals— A 2-year-old Warmblood stallion.
Methods— A metallic foreign body was removed from a talocrural joint using a 10-mm magnetic retriever under arthroscopic guidance. Preoperative radiographs were used to locate the intra-articular position of the foreign body.
Results— Six months after surgery, the horse was no longer lame.
Conclusions— A magnetic retriever can be used for arthroscopic removal of metallic foreign bodies from the equine talocrural joint.
Clinical Relevance— A magnetic retriever may be useful for arthroscopic retrieval of metallic foreign bodies from synovial cavities in horses, especially when direct observation is impeded by inflamed synovial villi and fibrin.