Effects of inbreeding on nodal root system morphology and architecture of white clover (<Emphasis Type="Italic">Trifolium repens</Emphasis> L.)

Plants of white clover (Trifolium repens L.) cultivar Crau, a self-fertile Crau genotype, and nine generations of inbred progeny were raised in sand culture in a glasshouse experiment. Digital images of the root systems were made and root morphological characteristics were determined on all the plants. Root architectural parameters were measured on the Crau parent and the S₁, S₄, S₆, and S₉ inbred lines. The clover roots became shorter and thicker with inbreeding but the number of root tips per plant was unchanged. Root architecture (branching pattern) was largely unaffected by inbreeding. It is concluded that inbreeding white clover will lead to shorter, thicker roots, and reduced nutrient uptake efficiency compared with the parent clover. The degree to which these deleterious traits are overcome during the development of F₁ hybrids needs to be determined.     

INTEGRATED CROP-LIVESTOCK SYSTEMS: LESSONS FROM NEW YORK,BRITISH COLUMBIA,AND THE SOUTH-EASTERN UNITED STATES

• Livestock production in North America has moved to fewer farms with greater inventories • Land application of livestock manures is a preferred nutrient recycling strategy • Confined animal feeding operations have challenges to utilize livestock manure sustainably • Integration of livestock and cropping systems is possible on a farm or among farms • Nutrient balance is needed for environmental sustainability Livestock production in the United States (US) and Canada is diverse, but shows a common trend in most livestock sectors toward fewer farms producing the majority of animal products despite a large number of farms still small in production scale. The migration to larger and more concentrated animal feeding operations in beef finishing and poultry, swine, and dairy production allows processors to streamline supplies to meet market demand for abundant, low-cost livestock products, whether that be for packaged meat, dairy products, or eggs. With concentration of livestock operations comes the challenge of managing manures. When sufficient land is available and nutrients are needed, livestock manure is an excellent nutrient source and land application is the preferred method of recycling this resource. However, when livestock production is constrained in a geographical area and animal densities are high, manure may become an environmental liability with potentially greater risk for runoff and leaching of nutrients, emission of odors, ammonia, and greenhouse gases, and release to the environment of pathogens and chemicals of emerging concern. Addressing these challenges now and into the future requires learning from mistakes and adopting successful approaches. We describe different levels of integration between livestock and crop producers in New York, British Columbia, and the south-eastern US as learning opportunities to improve economic and environmental sustainability. Examples show that effective solutions should recognize (1) manure has value and is not just a cost, (2) farmers, farm advisors, extension educators, nutrient management planners, crop advisors, nutritionists, state agency personnel, regulators, and university researchers need to be active participants in development of solutions, and (3) change to a sustainable future requires a combination of government regulation and outcome-based incentives.     

Microscopic analysis of the effect of azoxystrobin treatments on Mycosphaerella graminicola infection using green fluorescent protein (GFP)‐expressing transformants

Green fluorescent protein (GFP)‐expressing transformants were used to investigate the effects of strobilurin fungicide azoxystrobin on Mycosphaerella graminicola infection. Azoxystrobin treatments (125 or 250 g AI ha^?1) were applied at various stages of the infection process under controlled conditions. GFP transformants showed conserved in vitro sensitivity to azoxystrobin and pathogenicity. Azoxystrobin controlled over 90% of M graminicola infections when applied before or during penetration of the pathogen (15% of the incubation phase). Azoxystrobin also impaired the growth of intercellular hyphae in M graminicola post‐penetration infection stages when applied at up to 50% of the incubation phase. Incubating infections observed in treated leaves were viable, but their growth was impaired and they did not induce necrosis under controlled conditions. Reduction by half of azoxystrobin dosage had little or no effect on azoxystrobin efficiency in controlling M graminicola. The contribution of post‐penetration fungistatic effect to azoxystrobin curative properties toward M graminicola in a field situation is discussed. © 2001 Society of Chemical Industry     

The transmission by nematodes of tobraviruses is not determined exclusively by the virus coat protein

The coat protein gene of the nematode non-transmissible, SP5 isolate of pea early-browning tobravius was replaced with that of the highly nematode transmissible, PPK20 isolate of tobacco rattle tobravirus. Plants were infected with the recombinant virus when mechanically inoculated and the virus invaded the plants systemically. However, although the PPK20 isolate of TRV was transmitted by nematodes from these plants, the recombinant virus was not transmitted. Therefore, the virus coat protein is not the exclusive determinant of nematode transmission.     

Quantitative PCR monitoring of the effect of azoxystrobin treatments on Mycosphaerella graminicola epidemics in the field

Quantitative PCR and visual monitoring of Mycosphaerella graminicola epidemics were performed to investigate the effect of curative and preventative applications of azoxystrobin in wheat field crops. A non-systemic protectant and a systemic curative fungicide, chlorothalonil and epoxiconazole, respectively, were used as references. PCR diagnosis detected leaf infection by M graminicola 3 weeks before symptom appearance, thereby allowing a clear distinction between curative and preventative treatments. When applied 1 week after the beginning of infection, azoxystrobin curative activity was intermediate between chlorothalonil (low effect) and epoxiconazole. When applied preventatively, none of the fungicides completely prevented leaf infection. There was some indication that azoxystrobin preventative treatments may delay fungal DNA increase more than epoxiconazole at the beginning of leaf infection. Both curative and preventative treatments increased the time lapse between the earliest PCR detection and the measurement of a 10% necrotic leaf area. Azoxystrobin only slightly decreased the speed of necrotic area increase compared with epoxiconazole. Hence, azoxystrobin activity toward M graminicola mainly resides in lengthening the time lapse between the earliest PCR detection and the measurement of a 10% necrotic leaf area. Information generated in this way is useful for optimal positioning of azoxystrobin treatments on M graminicola.     

世界银行倡导以农业促发展

当今世界的农业发展概况 当今世界的农业,广袤、多样且快速变化.如果地方层面、国家层面乃至全球层面都有健全的政策和支持性投入,今天的农业将为数以亿计的农村穷人摆脱贫困提供新的契机.农业向他们敞开脱贫道路包括家庭种植业和养殖业、高附加值的"新型农业"就业机会、新兴的农村非农产业创业和就业机会等等.