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Socio‐economic factors related to management of costal zones and biological parameters related to species‐specific requirements set limitations for the rearing location of net‐pen sea cages. A cage was designed and installed at 45 m depth based on the REFA Tension Length Cage technology at the cage facility of the Hellenic Centre for Marine Research in Crete. The cage‐net module consisted of a net‐pen contained within a hexagonal rigid frame to maintain the shape of the net. Vertical beams of the frame glider on six tension legs were placed in a circle. The operation of the cage was evaluated using a group of red porgy (Pagrus pagrus) for a rearing period of 4 months whereas a second group reared in a surface net‐pen cage served as control. Growth performance was similar between the experimental groups. The rearing method significantly affected fish skin pigmentation. Individuals reared in the submerged cage had a brighter skin colour and lower skin melatonin content compared with the ones reared in the surface cage. The presented submerged cage may provide a feasible alternative for rearing fish in exposed areas and particularly species requiring specific biological conditions, such as narrow temperature ranges and low light intensity.  相似文献   
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Humans have more than doubled the amount of reactive nitrogen (Nr) added to the biosphere, yet most of what is known about its accumulation and ecological effects is derived from studies of heavily populated regions. Nitrogen (N) stable isotope ratios ((15)N:(14)N) in dated sediments from 25 remote Northern Hemisphere lakes show a coherent signal of an isotopically distinct source of N to ecosystems beginning in 1895 ± 10 years (±1 standard deviation). Initial shifts in N isotope composition recorded in lake sediments coincide with anthropogenic CO(2) emissions but accelerate with widespread industrial Nr production during the past half century. Although current atmospheric Nr deposition rates in remote regions are relatively low, anthropogenic N has probably influenced watershed N budgets across the Northern Hemisphere for over a century.  相似文献   
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