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排序方式: 共有69条查询结果,搜索用时 15 毫秒
1.
Penta Pristijono Konstantinos Papoutsis Christopher J. Scarlett Michael C. Bowyer Quan V. Vuong Costas E. Stathopoulos 《The Journal of Horticultural Science and Biotechnology》2017,92(5):521-529
Mature green tomatoes (Solanum lycopersicum L. cv Neang Pich) were exposed to 13.6 kJ m?2 UV-C or 0.5 μL L?1 1-MCP or combination of 13.6 kJ m?2 UV-C and 0.5 μL L?1 1-MCP, with appropriate untreated controls. After treatment, tomatoes were stored in air containing 0.1 μL L?1 ethylene at 20°C and 100% RH. The untreated fruit ripened significantly faster than those of all other treatments. UV-C treatment alone was able to delay fruit ripening by up to 5 days longer compared to untreated fruits whilst the additional of 1-MCP further delayed fruit ripening. UV-C and 1-MCP treatments alone or in combination had significantly slower ethylene production rates throughout the storage period. The fruit treated with the combination of 1-MCP and UV-C was significantly firmer and had higher total phenolic content compared to that of the other treatments. However, there was no difference between treatments in soluble solids content/titratable acids ratio, chlorophyll content, lycopene content and total antioxidant activity. These results show that UV-C and 1-MCP treatment delay ripening and improve the quality of tomatoes in the presence of low-level ethylene during storage. This new treatment could be used to extend the shelf-life of mature green tomatoes through the supply chain without the use of refrigeration. 相似文献
2.
Discrimination of three trachurus species using both mitochondrial- and nuclear-based DNA approaches
Karaiskou N Triantafyllidis A Triantaphyllidis C 《Journal of agricultural and food chemistry》2003,51(17):4935-4940
A double-DNA approach was developed to discriminate the three Trachurus species that abide in European waters: T. trachurus, T. mediterraneus, and T. picturatus. The analysis aimed at both mitochondrial and nuclear loci. Polymerase Chain Reaction (PCR) amplification of the cytochrome b gene of mtDNA was followed by restriction analysis with three species-specific enzymes: NlaIII, NciI, and BsmAI. Digestion with these endonucleases yielded species-specific electrophoretic profiles. The universality of the results was verified by screening a large number of individuals from 12 geographical regions covering most of the distribution of the species. Additionally, the nuclear multicopy 5S rRNA gene was selected as an alternative candidate for the discrimination of the three Trachurusspecies. A simple agarose gel electrophoretic analysis of the amplicons proved to be capable of leading to unambiguous identification of the three Trachurus species. Thus, the double-DNA methodology presented here allows the accurate discrimination of Trachurus fish species and the detection of commercial fraud. 相似文献
3.
Influence of age and purpose for testing on the cut-off selection of serological methods in bovine neosporosis 总被引:3,自引:0,他引:3
Alvarez-García G Collantes-Fernández E Costas E Rebordosa X Ortega-Mora LM 《Veterinary research》2003,34(3):341-352
The aim of this study was to investigate the need for different cut-off points, according to animal age and the purpose of testing, for two of the most widely used serological techniques in bovine neosporosis, IFAT and a crude antigen ELISA (Civtest, HIPRA). Therefore, the population reference sera used were defined using a combination of multiple criteria such as epidemiological/clinical and histopathological parameters and an immunoblot test. Firstly, foetuses and breeding cattle (heifers and cows) were considered as separate subpopulations for serological evaluation. Secondly, cut-off points for each serological technique (IFAT and ELISA) according to age group (foetuses and breeding cattle) and the different practical applications (detection of infection and abortion) were calculated following the receiver operating characteristics (ROC) analysis. Cut-off points were defined, for IFAT and ELISA for aborted breeding cattle and for IFAT alone in the case of the foetuses, assuming an equivalent cost of false positive and negative results. In infected breeding cattle, for IFAT and ELISA and in foetuses for ELISA, two possible cut-off values were obtained, one for a maximum sensitivity and one for a maximum specificity and the intervals of unclear results were defined. In this case, a cut-off value for equal sensitivity and specificity was also estimated. When cut-off points for infected breeding cattle, 1:100-1:250 for IFAT and 0.306-0.451 for ELISA were applied to a target population, optimal and similar negative and positive predictive values together with similar apparent and true prevalence results were observed suggesting the possibility of using both tests interchangeably. 相似文献
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6.
Fast mass transport through sub-2-nanometer carbon nanotubes 总被引:1,自引:0,他引:1
Holt JK Park HG Wang Y Stadermann M Artyukhin AB Grigoropoulos CP Noy A Bakajin O 《Science (New York, N.Y.)》2006,312(5776):1034-1037
We report gas and water flow measurements through microfabricated membranes in which aligned carbon nanotubes with diameters of less than 2 nanometers serve as pores. The measured gas flow exceeds predictions of the Knudsen diffusion model by more than an order of magnitude. The measured water flow exceeds values calculated from continuum hydrodynamics models by more than three orders of magnitude and is comparable to flow rates extrapolated from molecular dynamics simulations. The gas and water permeabilities of these nanotube-based membranes are several orders of magnitude higher than those of commercial polycarbonate membranes, despite having pore sizes an order of magnitude smaller. These membranes enable fundamental studies of mass transport in confined environments, as well as more energy-efficient nanoscale filtration. 相似文献
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8.
Cyanobacteria often produce severe illness and in some cases spectacular fatality on livestock and wildlife world-wide. Heavy cyanobacterial waterblooms usually form patches of dense surface scum, and terrestrial animals drinking such concentrated dirty froth can consume a fatal dose. Surprisingly, animals do not avoid swallowing concentrated microbial scum. Different experiments of drink selection were performed with laboratory mice to determine why animals drink these concentrated scum. These experiments showed that animals elected to consume dense cultures of the toxic cyanobacteria Microcystis aeruginosa in preference to limpid water. When M. aeruginosa cells were supplied ad libitum, mice avidly swallowed these toxic cyanobacteria until this led to their death. Mice were unable to detect the phycotoxin (microcystin). In contrast, mice did not select cultures containing other non-toxic phytoplanktonic organisms. Observations in nature suggest that this preference in the consumption of toxic cyanobacteria is common among other animal species. 相似文献
9.
Bruno Reis Ana Teresa Gonalves Paulo Santos Manuel Sardinha Luís E. C. Conceio Renata Serradeiro Jaume Prez-Snchez Josep Calduch-Giner Ulrike Schmid-Staiger Konstantin Frick Jorge Dias Benjamín Costas 《Marine drugs》2021,19(12)
This work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects. 相似文献
10.
Panagiotis J. Skouras George J. Stathas Costas Ch. Voudouris Anastasios I. Darras John A. Tsitsipis John T. Margaritopoulos 《Phytoparasitica》2017,45(2):165-173
Coccinella septempunctata L. is one of the most abundant ladybird species in Greece, preying on several aphid species and other arthropods, of which many are pests of cultivated plants. These pests are usually controlled with chemical insecticides. During this process, however, beneficials are also exposed to pesticides. The development of Integrated Pest Management (IPM) programs against aphids requires the evaluation of the effects of insecticides on beneficial insects. We evaluated the LD50 of imidacloprid, acetamiprid, bifenthrin and deltamethrin on first, second, third and fourth instar larvae of C. septempunctata by topical application. Moreover, we studied their sublethal effects (LD10) on the development, weight and prey consumption of fourth instar larvae. The topical application bioassays showed that deltamethrin and bifenthrin were highly toxic to all larval instars, whereas imidacloprid and acetamiprid were less toxic to fourth instar larvae. The LD10 dose significantly affected, developmental time, adult weight and daily predation. These results show the importance of assessing potential effect of insecticides on C. septempunctata for developing effective IPM programs of aphids in Greece. 相似文献