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Georg Lehner Monika Linek Ross Bond David H. Lloyd Ellen Prenger-Berninghoff Nina Thom Iris Straube Kristien Verheyen Anette Loeffler 《Veterinary microbiology》2014
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a highly drug-resistant small animal veterinary pathogen. Although often isolated from outpatients in veterinary clinics, there is concern that MRSP follows a veterinary-hospital-associated epidemiology. This study's objective was to identify risk factors for MRSP infections in dogs and cats in Germany. Clinical isolates of MRSP cases (n = 150) and methicillin-susceptible S. pseudintermedius (MSSP) controls (n = 133) and their corresponding host signalment and medical data covering the six months prior to staphylococcal isolation were analysed by multivariable logistic regression. The identity of all MRSP isolates was confirmed through demonstration of S. intermedius-group specific nuc and mecA. In the final model, cats (compared to dogs, OR 18.5, 95% CI 1.8–188.0, P = 0.01), animals that had been hospitalised (OR 104.4, 95% CI 21.3–511.6, P < 0.001), or visited veterinary clinics more frequently (>10 visits OR 7.3, 95% CI 1.0–52.6, P = 0.049) and those that had received topical ear medication (OR 5.1, 95% CI 1.8–14.9, P = 0.003) or glucocorticoids (OR 22.5, 95% CI 7.0–72.6, P < 0.001) were at higher risk of MRSP infection, whereas S. pseudintermedius isolates from ears were more likely to belong to the MSSP-group (OR 0.09, 95% CI 0.03–0.34, P < 0.001). These results indicate an association of MRSP infection with veterinary clinic/hospital settings and possibly with chronic skin disease. There was an unexpected lack of association between MRSP and antimicrobial therapy; this requires further investigation but may indicate that MRSP is well adapted to canine skin with little need for selective pressure. 相似文献
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Anette Moldestad Bernt Hoel Ulrike Böcker Shiori Koga Ellen Færgestad Mosleth Anne Kjersti Uhlen 《Journal of Cereal Science》2014
The aim of this study was to investigate effects of temperature during grain filling on gluten quality characteristics at a lower to moderate temperature range. Experiments with two wheat varieties grown in field covered by polypropylene tunnels during grain filling were performed in two seasons. Mean day temperature differences achieved within the tunnel were approximately 2–2.5 °C from the open to the closed end. There were significant effects of temperature on grain maturity time, thousand grain weight and protein content. The resistance to stretching of the gluten doughs increased with the increasing day temperatures. This was reflected in the proportion of unextractable polymeric proteins (UPP). The results suggest that increases in temperature within this temperature range affect the polymerization of polymeric proteins, giving higher molecular weights, and hence increased Rmax and stronger gluten. The two varieties differed in their response to temperature. In addition, there were seasonal variations in gluten functionality that may be associated with fluctuations in day temperatures between the seasons. 相似文献
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Lund E Engeset D Alsaker E Skeie G Hjårtaker A Lundebye AK Niebor E 《Science (New York, N.Y.)》2004,305(5683):477-8; author reply 477-8
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Habitat specificity indices reflect richness (α) and/or distinctiveness (β) components of diversity. The latter may be defined by α and γ (landscape) diversity in two alternative ways: multiplicatively () and additively (). We demonstrate that the original habitat specificity concept of Wagner and Edwards (Landscape Ecol 16:121–131, 2001) consists of three independent components: core habitat specificity (uniqueness of the species composition), patch area and
patch species richness. We describe habitat specificity as a family of indices that may include either area or richness components,
or none or both, and open for use of different types of mean in calculation of core habitat specificity. Core habitat specificity
is a beta diversity measure: the effective number of completely distinct communities in the landscape. Habitat specificity
weighted by species number is a gamma diversity measure: the effective number of species that a patch contributes to landscape
richness. We compared 12 habitat specificity indices by theoretical reasoning and by use of field data (vascular plant species
in SE Norwegian agricultural landscapes). Habitat specificity indices are strongly influenced by weights for patch area and
patch species richness, and the relative contribution of rare vs. common species (type of mean). The relevance of properties
emphasized by each habitat specificity index for evaluation of patches in a biodiversity context is discussed. Core habitat
specificity is emphasized as an ecologically interpretable measure that specifically addresses patch uniqueness while habitat
specificity weighted by species number combines species richness and species composition in ways relevant for conservation
biological assessment.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Anette Amtmann Ibrahim Ahmed Petra Zahner-Rimmel Adam Mletzko Lisa Katharina Jordan Martin Oberle Helmut Wedekind Jürgen Christian Sven Michael Bergmann Anna Maria Becker 《Journal of fish diseases》2020,43(2):185-195
In a search for alternative, environmentally friendly and effective disinfecting agents, a commercially available protease—Neutrase®—was tested in this work for inactivation of koi herpesvirus (KHV) and of viral haemorrhagic septicaemia virus (VHSV). For comparison, the stability of these viral pathogens in similar configurations at various pH values and concentrations of peracetic acid or quicklime, typically used for disinfection, was tested. Therefore, virus suspensions were incubated with various concentrations of different agents for 24 hr and the titre of the remaining infectious particles was determined by virus titration. Furthermore, the treatment of both viruses, with the agents at concentrations that were previously appointed as effective, was also examined in the presence of solid material (quartz sand). All procedures investigated in this study, including the protease treatment, were able to reduce the titre of KHV and VHSV below the detection limit of the titration. Although further studies are necessary, this is the first report of the application of a protease for the inactivation of the selected fish pathogens, demonstrating the great potential of the latter for disinfection. 相似文献
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The digestive tract of 15 elephants from South Africa and two from Zaïre were sampled in order to determine the identity, density and population composition of the intestinal protozoa. The following orders were represented: Gymnostomatida, Trichostomatida and Entodiniomorphida. Altogether 17 species were identified, of which three are new. The new species as well as a new genus are described. 相似文献
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Lasse Dam Rasmussen Giovanni Savini Alessio Lorusso Anna Bellacicco Massimo Palmarini Marco Caporale Thomas Bruun Rasmussen Graham J Belsham Anette B?tner 《Veterinary research》2013,44(1):75
Transplacental transmission of bluetongue virus has been shown previously for the North European strain of serotype 8 (BTV-8) and for tissue culture or chicken egg-adapted vaccine strains but not for field strains of other serotypes. In this study, pregnant ewes (6 per group) were inoculated with either field or rescued strains of BTV-2 and BTV-8 in order to determine the ability of these viruses to cross the placental barrier. The field BTV-2 and BTV-8 strains was passaged once in Culicoides KC cells and once in mammalian cells. All virus inoculated sheep became infected and seroconverted against the different BTV strains used in this study. BTV RNA was detectable in the blood of all but two ewes for over 28 days but infectious virus could only be detected in the blood for a much shorter period. Interestingly, transplacental transmission of BTV-2 (both field and rescued strains) was demonstrated at high efficiency (6 out of 13 lambs born to BTV-2 infected ewes) while only 1 lamb of 12 born to BTV-8 infected ewes showed evidence of in utero infection. In addition, evidence for horizontal transmission of BTV-2 between ewes was observed. As expected, the parental BTV-2 and BTV-8 viruses and the viruses rescued by reverse genetics showed very similar properties to each other. This study showed, for the first time, that transplacental transmission of BTV-2, which had been minimally passaged in cell culture, can occur; hence such transmission might be more frequent than previously thought. 相似文献
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From 2001 to 2005 the influence of the dwarfed apple rootstocks M 9 (clone: Burgmer 984), Fleuren 56, Supporter 1, P 16, P 22, J-TE-F, J-TE-E and M 27 on growth, yield and fruit size of the apple variety ‘Boskoop, Herr’ was investigated on the locations Auweiler, Dresden-Pillnitz, Osnabrück and Wurzen in Germany. All rootstocks induced a less vegetative growth as the rootstock M 9. In comparison with M 9 the crown volume of the trees ranged from 93% (Supporter 1) to 48% (P 22). The highest yield potential showed the rootstock P 16. Her specific yield (kg/m3) was 41% higher than the specific yield of M 9. J-TE-F produced the lowest specific yield. 相似文献
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