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排序方式: 共有793条查询结果,搜索用时 62 毫秒
1.
Brownlie J Nuttall PA Stott EJ Taylor G Thomas LH 《Veterinary immunology and immunopathology》1980,1(4):371-378
Certain immunological responses of 4-6 month old calves experimentally inoculated with either cytopathic or non-cytopathic bovine virus diarrhoea virus (BVDV) were compared with those of uninfected control calves. The tests used to demonstrate the immunological responses were the transformation of lymphocytes by PHA mitogen, the percentage of lymphocytes with surface immunoglobulin, and the antibody titres induced by an intravenous inoculation of killed Brucella abortus. There were no significant differences between the two groups of calves and therefore, the mild experimental disease produced by BVDV did not appear to affect adversely the immunological response. 相似文献
2.
Adrian Mutlow Ramiro Isaza James W Carpenter David E Koch Robert P Hunter 《Journal of zoo and wildlife medicine》2004,35(4):489-496
Using a crossover study design, the pharmacokinetics of carfentanil and naltrexone after i.v., i.m., and s.c. administration were determined in eight domestic goats (Capra hircus). Serial blood samples were taken up to 120 hr after carfentanil administration, and the plasma drug concentrations were determined using liquid chromatography and mass spectroscopy. All goats were immobilized with 40 microg/kg carfentanil i.m., although the resulting neurologic effects varied considerably. Plasma profiles showed rapid carfentanil absorption and a simple biphasic decline for 12-48 hr. Naltrexone given at 100 mg naltrexone/mg carfentanil 30 min after carfentanil administration produced rapid reversal of immobilization after all routes of administration. Variable fluctuations in the naltrexone plasma concentrations during the first 2.5-3.5 hr were observed, followed by a more consistent biphasic decline. The time to standing was significantly shorter after i.v. compared with s.c. naltrexone, although the time difference (1 min) had little clinical relevance. No statistically significant differences between the naltrexone pharmacokinetic parameters measured for the three routes of naltrexone administration were identified, although the recoveries after i.m. administration were, subjectively, the smoothest. The carfentanil half-life did not differ significantly in the goats given naltrexone by different routes. Although it is currently recommended that the naltrexone dose be divided into s.c. and i.v. portions, this practice does not appear to offer any benefit. 相似文献
3.
4.
Monjeau J. Adrian Birney Elmer C. Ghermandi Luciana Sikes Robert S. Margutti Laura Phillips Carleton J. 《Landscape Ecology》1998,13(5):285-306
Assemblages of plants were studied at 14 sites in northern Patagonia corresponding to localities at which we (Monjeau et al. 1997) earlier studied the relationship between small mammal assemblages and landscape classifications. This allowed us to test predictions that both plants and small mammals correspond to the more inclusive hierarchical landscape divisions but that plants track better than small mammals the less inclusive divisions. Species presence or absence of plants at each locality was used in a series of multivariate analyses and compared by correlation analysis with those generated from small mammal species data. Assemblages of both plants and small mammals corresponded to the upper divisions, which are based on climatic and geomorphological features, but small mammal assemblages did not correspond to the lower divisions of the landscape classifications. Three factors are considered as explanations for the observed differences between plants and small mammals: a) small mammal habitat is determined more by plant growth form than by plant species; b) trophic level differences between the two groups; and c) species pool size affects the resolution of microhabitat correspondence. Our data indicate that both plant assemblages and small mammal assemblages respond to climatic and geomorphological features, which is in contrast to the paradigm that mammal assemblages simply follow plant assemblages. We also attempted to reconcile classification systems in Patagonia by proposing a nomenclatural system based on a hierarchical classification. In the system proposed, ecoregion is the lowest division small mammal assemblages can recognize in Patagonia. Finally, we conclude that the hierarchical nature of landscapes based on a holistic view of environments reflects real entities that are not just the perceptions of landscape ecologists. 相似文献
5.
Isabelle Brodard Maher Alsaaod Corinne Gurtner Joerg Jores Adrian Steiner Peter Kuhnert 《Journal of veterinary diagnostic investigation》2021,33(4):801
Digital dermatitis (DD) is a major infectious foot disease of cattle worldwide. Some DD stages are associated with lameness, and the disease has significant economic and animal welfare consequences. The pathogenesis of the disease is not yet fully understood, but Treponema spp. have been associated consistently with clinical cases. Isolation of these fastidious bacteria is difficult and cumbersome. We describe an improved method enabling the culturing of the 3 Treponema spp. (T. pedis, T. phagedenis, and T. medium) from bovine foot specimens derived from DD lesions, using a combination of membrane filtering and subsequent growth on selective agar media. The entire procedure from sampling to verification of individual Treponema spp. takes up to 24 d. In addition, we established a MALDI-TOF MS–based identification method to be applied for confirmation of the different Treponema spp. This scheme provides an unambiguous, simple, and straightforward identification procedure for DD-associated Treponema spp. 相似文献
6.
Donovan DC Reber AJ Gabbard JD Aceves-Avila M Galland KL Holbert KA Ely LO Hurley DJ 《American journal of veterinary research》2007,68(7):778-782
OBJECTIVE: To assess the effect of maternal cells or cellular components on neonatal immune responses to intracellular pathogens in calves. ANIMALS: 15 Holstein calves. PROCEDURES: Calves were fed whole colostrum, frozen colostrum, or cell-free colostrum within 4 hours after birth. Leukocytes were obtained from calves before feeding colostrum and 1, 2, 7, 14, 21, and 28 days after ingestion. Proliferative responses against bovine viral diarrhea virus (BVDV) and mycobacterial purified protein derivatives were evaluated. Dams received a vaccine containing inactivated BVDV, but were not vaccinated against mycobacterial antigens. RESULTS: All calves had essentially no IgG in circulation at birth, but comparable and substantial concentrations by day 1. Calves that received whole colostrum had enhanced responses to BVDV antigen 1 and 2 days after ingestion of colostrum. In contrast, calves that received frozen colostrum or cell-free colostrum did not respond to BVDV. No differences were identified among the 3 groups in response to mycobacterial antigens. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that transfer of live maternal cells from colostrum to neonatal calves enhanced responses to antigens against which the dams had previously responded (BVDV), but not to antigens to which the dams were na?ve (mycobacterial purified protein derivatives). Results suggested that cell-mediated immune transfer to neonates can be enhanced by maternal vaccination. 相似文献
7.
Pfeiffer JB Mevissen M Steiner A Portier CJ Meylan M 《American journal of veterinary research》2007,68(3):313-322
OBJECTIVE: To describe the in vitro effects of bethanechol on contractility of smooth muscle preparations from the small intestines of healthy cows and define the muscarinic receptor subtypes involved in mediating contraction. SAMPLE POPULATION: Tissue samples from the duodenum and jejunum collected immediately after slaughter of 40 healthy cows. PROCEDURES: Cumulative concentration-response curves were determined for the muscarinic receptor agonist bethanechol with or without prior incubation with subtype-specific receptor antagonists in an organ bath. Effects of bethanechol and antagonists and the influence of intestinal location on basal tone, maximal amplitude (A(max)), and area under the curve (AUC) were evaluated. RESULTS: Bethanechol induced a significant, concentration-dependent increase in all preparations and variables. The effect of bethanechol was more pronounced in jejunal than in duodenal samples and in circular than in longitudinal preparations. Significant inhibition of the effects of bethanechol was observed after prior incubation with muscarinic receptor subtype M(3) antagonists (more commonly for basal tone than for A(max) and AUC). The M(2) receptor antagonists partly inhibited the response to bethanechol, especially for basal tone. The M(3) receptor antagonists were generally more potent than the M(2) receptor antagonists. In a protection experiment, an M(3) receptor antagonist was less potent than when used in combination with an M(2) receptor antagonist. Receptor antagonists for M(1) and M(4) did not affect contractility variables. CONCLUSIONS AND CLINICAL RELEVANCE: Bethanechol acting on muscarinic receptor sub-types M(2) and M(3) may be of clinical use as a prokinetic drug for motility disorders of the duodenum and jejunum in dairy cows. 相似文献
8.
Adrian C. Armstrong Andrew M. Portwood Peter B. Leeds-Harrison Graham L. Harris John A Catt 《Pest management science》1996,48(1):47-55
The validation of pesticide leaching models presents particular problems where the number of model predictions is far in excess of the observed data. Normally, however, there are more frequent field observations for other parameters (notably the site hydrology) than for pesticide concentrations in either water or soil. A five-stage validation procedure which takes advantage of the most frequently available observations and which tests each of the components of the model in a cumulative way, is thus advocated: Stage 1: Parameterisation of the model using only independently measured parameters. Stage 2: Hydrological validation: the validation of the predictions of water movement and water content of the soil. Stage 3: Solute movement validation: where field data are available for solutes other than pesticide, the model should first be validated for them, especially if they are more abundant than the pesticide observations. Conserved solutes such as chloride or bromide are preferred, although nitrate may be used for short periods. Stage 4: Pesticide fate in the soil: models should use parameters of pesticide fate derived from independent studies. Stage 5: Pesticide leaching: only in the last stage are the relatively small number of pesticide observations compared with the model predictions with respect to patterns and orders of magnitude of occurrence. With this scheme, the results of each stage are carried forward to the next, and confidence in the model is built with each stage. This is illustrated using the CRACK-P model and hydrological, nitrate and pesticide data from the Brimstone Farm Experiment Oxfordshire, UK. 相似文献
9.
Septoria leaf blotch progresses rapidly, leading to the development of Zymoseptoria titici forms resistant to fungicides. Cephalosporium stripe is caused by Cephalosporium gramineum. The aim of this study was to evaluate the effectiveness of selected pesticides in limiting the symptoms of both diseases on winter wheat leaves, and to determine their influence on grain yield and the content and composition of protein fractions in wheat kernels. Propiconazoles were most effective in inhibiting the development of Septoria leaf blotch (symptoms were reduced from 54.7% to 78.6%). Strobilurins were less effective due to the presence of isolates with the G143A mutation. Symptoms of Cephalosporium stripe were rarely observed, and protective treatments did not reduce their severity. The highest content of grain protein (14.81%) was found in plants most intensely protected with the fungicides containing fenpropimorph, pyraclostrobin and epoxiconazole. The principal component analysis revealed that the plant protection method influenced the grain protein profile. The accumulation of HMW glutenins and α/β gliadins was mutually interrelated and higher in high-input treatments; control grain was characterized by close relationships between ω-gliadins, LMW glutenins, albumins and globulins, whereas low-input treatments influenced mostly γ-gliadins. 相似文献
10.
Dated shorelines of late Pleistocene pluvial Lakes Lahontan (Great Basin Desert, northwest Nevada) and Mojave (Mojave Desert, eastern California) provide timelines for the assessment of alluvial fan sedimentation at the lake margins during the late Pleistocene to early Holocene. Two sets of alluvial fan systems have been mapped: the Stillwater fans, feeding Lake Lahontan; and the Zzyzx fans, feeding Lake Mojave. Their contrasting morphologies suggest different responses of the two fan systems to late Pleistocene to early Holocene climatic change. At the time the Stillwater fan systems underwent minimal sedimentation, with the catchment hillslopes apparently stable. The Zzyzx fans experienced major changes in water and sediment supply from the catchment hillslopes. There was a major phase of hillslope debris-flow activity, followed by fanhead trenching and distal fan progradation. Both areas were wetter and colder in the late Pleistocene than they are today, but during the transition to the Holocene the Zzyzx area was more likely to experience intense rains associated with the monsoonal penetration of warm moist tropical air into the Southwest. Vegetation reconstructions for the late Pleistocene to the early Holocene suggest that catchment hillslopes in the Mojave supported a desert shrub vegetation, but those in the Stillwaters supported juniper woodland and grasses at low elevations and pine at higher elevations. Contrasts in hillslope vegetation cover together with storm activity may account for the different responses of the alluvial fans to climatic change during the Pleistocene to Holocene climatic transition. After the falls in lake levels of Lakes Lahontan and Mojave in the early Holocene, both areas underwent aridification, resulting in reductions in hillslope vegetation cover. Increased storm runoff led to fanhead trenching and distal progradation of the alluvial fans. Variations in fan style at that time may relate primarily to base-level conditions resulting from different gradients on the exposed lake shores. 相似文献