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为了开发高生物活性的稀土氨基酸配合物抑菌剂,在酸性乙醇介质中,合成了稀土镝(Dy3+)氨基酸(Tyr,Trp)咪唑三元配合物,由其一般性质、荧光性能及紫外吸收光谱得到初步确认。以Dy3+、咪唑及镝氨基酸二元配合物为对照,研究了镝氨基酸咪唑三元配合物的抑菌性能。结果表明,镝氨基酸二元及镝氨基酸咪唑三元配合物对大肠杆菌及金黄色葡萄球菌均有不同程度的抑制作用,咪唑参与配位后,更加强了配合物的抗菌性能,当两类配合物浓度≥8 g/L时,对两种供试菌均有较强的抑制作用;镝酪氨酸配合物抑菌作用较镝色氨酸配合物强,其最低抑菌浓度可低至2~4 g/L。 相似文献
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机油降解菌的分离及其降解特性研究 总被引:2,自引:0,他引:2
[目的]分离主要的机油降解菌,并研究其降解特性。[方法]从茂名炼油厂附近长期被石油污染的土壤中,分离机油降解菌株,并对其进行形态特征和生理生化特性的分析。同时进一步研究了该菌株对机油降解特性及影响因素。[结果]分离得到3株机油降解菌,其中1株初步鉴定为芽孢杆菌属。该菌株在温度为40℃、pH值为8.0、机油浓度为100mg/L、N源为NH4Cl的条件下生长旺盛,降解率达到47.2%。[结论]利用生物降解的方法可以更有效地治理石油污染。 相似文献
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茄类内生菌的分离及拮抗细菌的筛选(英文) 总被引:2,自引:0,他引:2
[Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [Method] According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified endophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae. 相似文献
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[目的]分离番茄、辣椒和茄子3种茄科果蔬的内生菌,并对青枯病拮抗细菌进行筛选与鉴定。[方法]采用研磨液培养法,利用选择培养基对3种植株进行内生菌分离,并采用平板划线法纯化,及菌块对峙培养法对已纯化的内生菌进行筛选。根据分离菌的培养特性及菌体形态特征、革兰氏染色及生化反应等,对筛选出来的内生拮抗细菌进行鉴定归属。[结果]3种植株内生菌分离,共得到53株内生细菌、53株内生真菌、44株内生放线菌。筛选出14株青枯病菌有强拮抗性的内生细菌,分属为芽孢杆菌属、埃希氏菌属、克雷伯氏菌属、土壤单胞菌属、欧文氏菌属、短小杆菌属,其中芽孢杆菌为优势种群,而且芽孢杆菌属菌株对青枯病菌的拮抗作用最强。[结论]提供了探索生物防治茄科植物青枯病的有效途径。 相似文献
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