群井开采对毛乌素沙地地下水位的影响——以榆林市榆阳区昌汗敖包村土地开发项目为例

为探究毛乌素沙地大规模现代化高标准农田地下水开采对地下水位的影响，以榆林市榆阳区昌汗敖包村土地开发项目地下水监测网覆盖区作为研究区，选取分布于研究区的8眼机井进行了为期1年的地下水位埋深动态特征监测。结果表明:在灌溉期间，地下水位埋深呈先减小后增大的趋势；灌溉结束至进入下个灌溉周期之前，地下水位埋深逐渐增大至正常值。因此群井开采并未对项目区地下水位造成实质影响。      

氮吸附法分析生物质焦孔隙结构

采用氮吸附法对4种生物质焦(稻壳、树叶、玉米秆、棉花秆)的孔隙结构进行测量,结果表明,不同种类焦样的比表面积和孔径分布有明显差别,树叶的比表面积最大,为242.21 m2·g-1,玉米秆的比表面积最小,为0.81 m2·g-1.850℃时,稻壳、树叶、玉米秆焦样的孔径分布曲线在微孔和中孔范围各有一个分布峰,而棉花秆焦样的孔径分布曲线只在中孔范围内出现一个分布峰.热解温度是影响孔隙结构的一个重要因素,在高温条件下,同步热解得到的焦样的比表面积较大,微孔较多.在本研究中,600℃、850℃的稻壳焦样和850℃的树叶焦样具有较大的比表面积,比较适合做吸附剂.     

莫能菌素浓度对混合瘤胃微生物体外发酵的影响

本试验研究了莫能菌素对混合瘤胃微生物体外发酵的影响 ,以期确定莫能菌素在瘤胃内容物中的适宜添加浓度。混合瘤胃微生物由饲喂花生秧基础日粮的阉公牛瘤胃内容物和瘤胃液制备 ,用简易人工瘤胃装置测定莫能菌素浓度对产气量、瘤胃乙丙酸比例、粗纤维降解率和真蛋白产量的影响。结果表明 ,莫能菌素浓度强烈地抑制产气量 ,两者呈线性负相关(P<0.05);粗纤维降解率开始时随莫能菌素浓度的升高而增加 ,在浓度为0.078mg/L时达到最大值 ,两者呈二次幂函数关系 ;添加莫能菌素降低了瘤胃乙丙酸比例 ,但瘤胃乙丙酸比例随莫能菌素浓度的提高有逐渐增加的趋势 ;在莫能菌素浓度为0.1～2.0mg/L的范围内 ,添加莫能菌素对蛋白质的产量没有明显影响     

茶黄素类抗肿瘤作用研究进展

简述了茶黄素类(Theaflavins，TFs)的化学结构，并从抗肿瘤、抗炎、抗氧化、抗病毒和抗心血管疾病五个方面综述了茶黄素类的药理作用，其中从肿瘤的发生、发展两个阶段着重分析了茶黄素类的作用机理，最后提出了TFs作为药品开发所存在的问题。     

鸭大肠杆菌病流行病学与病原特性研究

对浙江等地鸭大肠杆菌病流行病学进行调查，并研究其发病特点，分离鉴定出致病性大肠杆菌26株；已对其中20株菌株进行血清型鉴定，主要为078，占70%（14/20），其次是O1，占15%（3/20）；人工感染试验表明，80%（8/10）菌株毒力强，皮下、肌肉、足蹼3种途径均易复制该病；病理组织学检查肝、肺、脾等，表现急性炎症，出血等病变；药敏试验表明，不同菌株对药物敏感性差异较大，除对鸭菌消、卡那霉素等高敏外，对多数常用抗菌药已耐药，最后就该病的防治提出了对策。     

知识管理在医院图书馆人力资源管理中的应用

 

根据知识管理的概念与内涵，从以人为本的角度，探讨了医院图书馆人力资源的特点以及实

施知识管理的具体做法。

 

 

     

猪血浆蛋白(酶)多态性与杂种优势的关系

为探讨血浆蛋白 (酶 )多态性与杂种优势的关系 ,测定了杜洛克、长白、大白、杜×长大、大×长大、长×大、大×长共 7个品种 (组合 )的 8个血浆蛋白 (酶 )位点的多态性及部分生长和胴体性状 ,计算了平均基因杂合度与部分经济性状实测值和杂优率的相关关系 .结果表明 ,平均基因杂合度与遗传距离呈正相关 ,与日增重、屠宰率、背膘厚、后腿比例的实测值或杂优率呈正相关 ,与眼肌面积的实测值和杂优率呈负相关 .平均基因杂合度和亲本间遗传距离可为预测杂种优势提供依据 .     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.