Expression of reproductive-related genes and changes in oocyte maturation of goldfish broodstock (Carassius auratus) following injection of different exogenous kisspeptins

Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 μg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis.     

Rabies Infection: An Overview of Lyssavirus-Host Protein Interactions

Viruses are obligatory intracellular parasites that use cell proteins to take the control of the cell functions in order to accomplish their life cycle. Studying the viral-host interactions would increase our knowledge of the viral biology and mechanisms of pathogenesis. Studies on pathogenesis mechanisms of lyssaviruses, which are the causative agents of rabies, have revealed some important host protein partners for viral proteins, especially for most studied species, i.e. RABV. In this review article, the key physical lyssavirus-host protein interactions, their contributions to rabies infection, and their exploitation are discussed to improve the knowledge about rabies pathogenesis. Key Words: Host-Pathogen Interactions, Lyssavirus, Rabies     

Analysis of hydrological drought characteristics using copula function approach

Paddy and Water Environment - Drought is a natural phenomenon which starts with decreased precipitation and can disrupt the environmental systems by changing the hydrological cycle. This is more...     

Three-dimensional finite element modelling of heat transfer for linear friction welding of Scots pine

Finite element numerical analyses were performed to determine three-dimensional heat flux generated by friction to wood pieces during linear friction welding. The objective was to develop a computational model to explain the thermal behaviour of welded wood material rather than experimental methods, which are usually expensive and time consuming. This model serves as a prediction tool for welding parameters, leading to optimal thermo-mechanical performance of welded joints. The energy produced by the friction welding of small wood specimens of Scots pine (Pinus sylvestris L.) was determined by thermocouples and used as input data in the model. The model is based on anisotropic elasticity and the thermal properties were modelled as isotropic. This numerical simulation gave information on the distribution of the temperature in the welding interface during the entire welding process. A good agreement between the simulation and experimental results showed the appropriateness of the model for planning welded wood manufacture and prediction of thermal behaviour of wood during other mechanically induced vibration processes. The specimens presented in this model required a heat flux of 11 kW/m² to achieve a satisfactory welding joint.     

Relationship between branch-scar parameters and knot features of oriental beech (Fagus orientalis Libsky)

The classification of roundwood is inextricably linked to the measurement of a particular single wood defect. The appearance, location, and number of defects are important in the quality evaluation of logs and sawn timber, and the most important defects are knots. The purpose of this study was to investigate the relationship between the appearance of branch scars and features of the related knot inside oriental beech logs, and to model the relationship between well-defined branch-scar and knot parameters. One hundred and fifty knots in 15 stems of oriental beech trees were studied. Image analysis software was used to measure the branch-scar and knot features. The results showed a significant positive correlation between the branch-scar parameter “moustache length” and the knot length. The ratio of branch-seal length to width was found to be a good estimator of the stem diameter at the time of knot occlusion and the amount of clear wood between the knot occlusion and the bark. The relationship obtained for the oriental beech stem radius at time of knot occlusion confirms relationship reported for European beech (Fagus sylvatica L.).