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排序方式: 共有357条查询结果,搜索用时 31 毫秒
1.
Goldstein RE Atwater DZ Cazolli DM Goldstein O Wade CM Lindblad-Toh K 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(1):199-203
BACKGROUND: Primary hyperparathyroidism (PHPT) is caused by inappropriate secretion of parathyroid hormone (PTH) by autonomously functioning neoplastic or hyperplastic parathyroid "chief" cells. Keeshonden are thought to be over-represented in studies on canine PHPT, but no proof of heritability or mode of inheritance has been published. The canine disease clinically resembles human familial isolated hyperparathyroidism (FIHP). HYPOTHESIS: Primary hyperparathyroidism in Keeshonden is genetically transmitted and is caused by a mutation in 1 of 4 genes implicated in human FIHP: MEN1, CASR, HRPT2, or RET. ANIMALS: Pedigrees consisting of 1647 Keeshonden were created including 219 Keeshonden with known PHPT phenotypes (69 positive). DNA samples were obtained from 176 of the 219 Keeshonden (34 positive). METHODS: Heritability and mode of inheritance were determined by segregation analysis. Canine homologs to the human genes were identified. Exons and surrounding intron regions were sequenced and scanned for sense-altering polymorphisms or polymorphisms that segregated with the disease. Messenger RNA from a parathyroid tumor of an affected Keeshond was analyzed for polymorphisms and splice alterations. RESULTS: PHPT follows an autosomal dominant mode of inheritance in Keeshonden with possible age-dependent penetrance. No polymorphisms identified in the genes analyzed were associated with a change in predicted protein or in hypothesized splice sites. CONCLUSIONS AND CLINICAL IMPORTANCE: PHPT is an autosomal dominant, genetically transmitted disease in Keeshonden. Once the mutation locus is identified, genetic testing should quickly decrease the incidence of PHPT in this breed. It is unlikely that mutations in MEN1, CASR, HRPT2, or RET cause PHPT in Keeshonden. 相似文献
2.
Tracey Jones Ruth Feber Gabriel Hemery Paul Cook Katy James Curt Lamberth Marian Dawkins 《Agricultural Systems》2007
The commercial, welfare and environmental impacts of rearing small colonies of free-range chickens in newly planted woodland at two sites in the UK, during the first 2 years of the enterprise, were investigated. Newly planted rather than established trees were used so that commercially viable species could be planted in a way that would suit the management of the chicken enterprise, and small colonies were used so that farmers wishing to diversify could buy into the system progressively. It was anticipated that marketing a high specification product would provide an immediate income, whilst waiting for the future investment in trees to grow. 相似文献
3.
Luke A. J. Haydock Anthony C. G. Abrams-Ogg J. Scott Weese Michael R. Goldstein Amy B. Clifford Adrian Sebastian Elizabeth H. Rea Frances B. Jamieson Carla Duncan Olga Andrievskaia Mirjana Savic Durda Slavic Robert A. Foster Christopher J. Greenwood Tamara L. MacDonald Jacqueline E. Scott Andrea Sanchez 《Journal of veterinary diagnostic investigation》2022,34(2):292
A 4-y-old, female mixed-breed dog was presented to the Ontario Veterinary College for further evaluation of multiple pulmonary and hepatic masses, intrathoracic lymphadenitis, and recent development of a pyogranulomatous pleural effusion. Along with other comprehensive tests, a thoracic lymph node biopsy was performed, and Mycobacterium tuberculosis complex infection was confirmed by real-time PCR. The dog’s condition declined post-operatively, and euthanasia was elected. Postmortem examination confirmed severe granulomatous pneumonia, hepatitis, intrathoracic and intraabdominal lymphadenitis, omentitis, and nephritis. Line-probe assays performed on samples collected postmortem confirmed the species as M. tuberculosis. 24-loci MIRU-VNTR genotyping, spoligotyping, and whole-genome sequencing revealed relations to known human isolates, but no epidemiologic link to these cases was investigated. Given the concern for potential human exposure during this animal’s disease course, a public health investigation was initiated; 45 individuals were tested for M. tuberculosis exposure, and no subsequent human infections related to this animal were identified. Our case highlights the need for more readily available, minimally invasive testing for the diagnosis of canine mycobacteriosis, and highlights the ability of canid species to act as potential contributors to the epidemiology of M. tuberculosis infections. 相似文献
4.
Nowakowski A. Justin Peaden J. Mark Tuberville Tracey D. Buhlmann Kurt A. Todd Brian D. 《Landscape Ecology》2020,35(4):893-906
Landscape Ecology - Thermal traits likely mediate organismal responses to changing thermal environments. As temperatures increase, predicting species responses will depend on understanding how... 相似文献
5.
6.
S K Sundar P K Lai Z H Bengall J L Cicmanec K Goldstein P H Levine 《Comparative immunology, microbiology and infectious diseases》1981,4(1):35-45
White-lipped marmosets were evaluated for their cell mediated immune (CMI) response to EBV to determine the feasibility of CMI studies in marmoset models for EBV oncogenesis. The mitogen, cell concentrations, the length of incubation period and serum requirements were defined for in vitro lymphocyte stimulation tests. The level of response of each animal was dependent on the concentration of phytohemagglutinin-P (PHA-P) and was independent of cell densities employed. The rate of tritiated-thymidine incorporation by mononuclear cells due to PHA-P increased exponentially between 2–4 days. This test was reproducible for a given batch of PHA-P when the cells were cultured in the presence of 10% heat inactivated fetal bovine serum. The five white-lipped marmosets were seronegative for EBV antigens and did not show lymphocyte stimulation with EBV particles and EBV soluble antigen, but two of these animals exhibited significant stimulation with autologous lymphocytes transformed in vitro by B95-8 virus. Despite the limited amount of blood (3–4 ml) that could be obtained from each animal in a single bleeding, it was possible to perform multiple lymphocyte stimulation assays with the protocol used. 相似文献
7.
Baneth G Volansky Z Anug Y Favia G Bain O Goldstein RE Harrus S 《Veterinary parasitology》2002,105(2):173-178
Therapy of canine dirofilariois due to Dirofilaria repens is indicated for dogs suffering from clinical signs of this disease, such as dermal swelling, sub-cutaneous nodules and pruritus. It is also important in order to decrease the risk of infection to other dogs and humans in the vicinity of the infected animal when suitable mosquito vectors are present. Combined therapy with the arsenic adulticide melarsomine and the avermectin microfilaricidal doramectin was effective in clearing infection with D. repens in a dog. The number of microfilariae dropped from 17 microl(-1) blood pre-treatment to 7 microl(-1) following the first adulticide injection and reached 0 a day after the microfilaricidal administration. The dog remained negative for D. repens microfilaremia during a follow-up period of 90 days. Euthanasia and necropsy performed 3 months after the initiation of therapy due to a progressive neoplastic disease revealed no evidence of filariae. 相似文献
8.
Leigh Ann Clayton Tracey K Ritzman 《Veterinary Clinics of North America: Exotic Animal Practice》2006,9(3):511-518
A 6-year-old cockatiel was diagnosed with egg binding based on clinical signs, physical examination findings, radiographs, and lack of oviposition. Medical management failed to induce oviposition, and surgical salpingotomy was used to remove the egg from the oviduct. Sterile egg yolk peritonitis was diagnosed based on histology. Postoperative management with leuprolide acetate and husbandary changes successfully prevented egg production for at least 2.5 months after the event. 相似文献
9.
Identification of functional corridors with movement characteristics of brown bears on the Kenai Peninsula,Alaska 总被引:2,自引:1,他引:2
Tabitha A. Graves Sean Farley Michael I. Goldstein Christopher Servheen 《Landscape Ecology》2007,22(5):765-772
We identified primary habitat and functional corridors across a landscape using Global Positioning System (GPS) collar locations
of brown bears (Ursus arctos). After deriving density, speed, and angular deviation of movement, we classified landscape function for a group of animals
with a cluster analysis. We described areas with high amounts of sinuous movement as primary habitat patches and areas with
high amounts of very directional, fast movement as highly functional bear corridors. The time between bear locations and scale
of analysis influenced the number and size of corridors identified. Bear locations should be collected at intervals ≤6 h to
correctly identify travel corridors. Our corridor identification technique will help managers move beyond the theoretical
discussion of corridors and linkage zones to active management of landscape features that will preserve connectivity. 相似文献
10.
Potential errors in measurement of nonuniform sap flow using heat dissipation probes 总被引:1,自引:0,他引:1
The empirical calibration of Granier-type heat dissipation sap flow probes that relate temperature difference (DeltaT) to sap velocity (v) was reevaluated in stems of three tropical tree species. The original calibration was confirmed when the entire heated probe was in contact with conducting xylem, but mean v was underestimated when part of the probe was in contact with nonconducting xylem or bark. Analysis of the effects of nonuniform sap velocity profiles on heat dissipation estimates showed that errors increased as v and the proportion of the probe in nonconducting wood increased. If half of a 20-mm probe is in sapwood with a v of 0.15 mm s(-1) and the other half is in nonconducting wood, then mean v for the whole probe can be underestimated by as much as 50%. A correction was developed that can be used if the proportion of the probe in nonconducting wood is known. Even with the entire heated probe in contact with conducting xylem, v would be underestimated when radial velocity gradients are present. In this case, the error would be smaller except when velocity gradients are very steep, as can occur in species with ring-porous wood anatomy. Errors occur because the relationship between DeltaT and v is nonlinear. Mean DeltaT along the probe is therefore not a measure of mean v, and users of heat dissipation probes should not assume that v is integrated along the length of the probe. The same type of error can occur when DeltaT is averaged through time while v is changing, but the error is small unless there are sudden, step changes between zero and high sap velocity. It is recommended that relatively short probes (20 mm or less) be used and that probes longer than the depth of conducting sapwood be avoided. Multiple probes inserted to a range of depths should be used in situations where steep gradients in v are expected. If these conditions are met, heat dissipation probes remain useful and widely applicable for measuring sap flow in woody stems. 相似文献