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排序方式: 共有219条查询结果,搜索用时 15 毫秒
1.
Hideki OGISO Masashi FUJINAGA Hideki SAITO Toshiaki TAKEHARA Shigeru YAMANAKA 《Journal of General Plant Pathology》2002,68(4):292-299
One hundred and sixteen isolates of Fusarium oxysporum f. sp. lactucae obtained from 85 fields in three crisphead lettuce-producing areas in Nagano Prefecture, Japan were typed for races using
differential cultivars Patriot, Banchu Red Fire and Costa Rica No. 4. They were also grouped into vegetative compatibility
groups (VCGs) using complementation tests with nitrate non-utilizing (nit) mutants. Two California strains reported as F. oxysporum f. sp. lactucum, a type culture of F. oxysporum f. sp. lactucae, and 28 avirulent isolates of F. oxysporum obtained from crisphead lettuce were included for comparison. Among Nagano isolates, 66 isolates were identified as race 1,
and 50 as race 2. Race 1 strains derived from Shiojiri and Komoro cities and race 2 from Kawakami village and Komoro city.
All isolates of race 2 were biotin auxotrophs, and the race could be distinguished based on its requirement for biotin on
minimal nitrate agar medium (MM). Pathogenic isolates were classified into two VCGs and three heterokaryon self-incompatible
isolates. Strong correlations were found between race and VCG. All the race 1 strains were assigned to VCG 1 except self-incompatible
isolates, and all the race 2 strains to VCG 2. The 28 avirulent isolates of F. oxysporum were incompatible with VCG 1 and VCG 2. California strains was vegetatively compatible with VCG 1, and they were assigned
to race 1. Based on vegetative compatibility, these two races of F. oxysporum f. sp. lactucae may be genetically distinct, and F. oxysporum f. sp. lactucae race 1 is identical to F. oxysporum f. sp. lactucum.
Received 7 May 2002/ Accepted in revised form 6 September 2002 相似文献
2.
Landscape enhancement projects are under way at the Yamanakadani and Kadowaki rivers, which run through the campus of Hiroshima University, Japan. At both sites, the ecological value of land was determined from two aspects: (1) value as vegetation and (2) value to birds. To evaluate the vegetation, we selected conservation sites and suitable sites for enhancement considering rarity and recovery potential of vegetation, and access to users and construction equipment. We determined that the area of forest, the number of forest vertical layers, and forest pattern help sustain avian diversity and contribute toward the area functioning as an avian corridor. 相似文献
3.
Asada Yohei Kimura Masaomi Azechi Issaku Iida Toshiaki Kubo Naritaka 《Paddy and Water Environment》2019,17(3):351-358
Paddy and Water Environment - Leakage of agricultural pipes has been increasingly frequent problems due to corrosion, loosening of joints, and cracks, which result in the subsidence of peripheral... 相似文献
4.
We have evaluated four commercially available mold starters (SP-01, NY, M1, and “for kome miso”) for fermented fish pastes with the aim of determining the effect of different koji molds on the nutritional and sensory characteristics of the fermented product. Comparative studies among different fermented
fish and bean pastes were also conducted. Protein contents were considerably higher in the fermented fish pastes (22.7–24.1%)
than the fermented bean pastes (8.2–12.8%). Free amino acids, oligopeptides, organic acids, mineral content, and sensory scores
of the fermented fish pastes were superior to those of the bean pastes in terms of nutritional aspects. Sensory evaluation
of fermented fish and bean pastes clearly indicated that different koji molds used as starters had little effect on the taste of the fermented fish pastes. However, in terms of nutritional and
sensory characteristics, fermented fish pastes from trash fishes were found to be both commercially valuable and a potential
gain to food processing firms among the many new generic fermented food products becoming available. 相似文献
5.
Takanobu Goto Shusaku Takagi Toshiaki Ichiki Tadashi Sakai Makoto Endo Terutoyo Yoshida Masaharu Ukawa Hisashi Murata 《Fisheries Science》2001,67(1):58-63
SUMMARY: The aim of this study was to investigate the cause of green liver in red sea bream fed substitute protein diets. Red sea bream Pagrus major was given either of the following diets for 28 weeks: (1) control diet (50% fish meal), (2) low level fish meal diet (15% fish meal), and (3) non-fish meal diet (0% fish meal). The green liver was observed in all groups tested, but the incidence was much higher in the experimental diet groups. The feeding of substitute protein diets reduced plasma triglyceride and cholesterol levels. However, there was no significant difference in plasma hepatic enzyme activities and plasma bile salts concentration among the treatments. Fish fed the substitute protein diets showed low hepatic taurine levels with an appearance of a biliverdin in the liver. Moreover, the proportion of ditaurobilirubin to total biliary bile pigments was significantly lower in fish fed the substitute protein diets. These data indicate that feeding of substitute protein diets did not induce any cholestatic hepatobiliary obstructions and that the low hepatic taurine level was one of the probable factors responsible for the occurrence of green liver in red sea bream fed substitute protein diets. 相似文献
6.
7.
Nitrous oxide (N2O) emission from flooded rice paddy fields was continuously measured by the closed chamber method at an experimental plot
in Thailand for a whole cultivation period. To characterize the N2O emission with regard to the denitrification N loss, the C2H2 inhibition method was applied. Flood water on the soil greatly suppressed the N2O emission. The N2O emission was mitigated considerably by even a thin film of the flood water. The overall average N2O emissions under flood conditions for one crop season (83 days) at the control site and the C2H2 treated site were 10.3 and 11.8 μg N m−2 h−1, respectively. The N2O emission from the C2H2 treated site was consistently higher than that from the control site and the N2O emission from both sites followed the same diurnal and seasonal variation pattern, indicating the effect of denitrification
inhibition by the supplied C2H2. The N2O emission enhanced along with temperature increase when NO3–N concentration in the soil water was above 0.4 mg N l−1 and soil temperature was above 24°C, suggesting specific temperature influence over the emission. The increase in NO3–N concentration and temperature in the soil affected only the N2O emission while the difference in the emission at the C2H2 treated site and the control site was not so much affected. It was suggested that most of the actively produced N2O under higher NO3–N concentration and temperature would likely to quickly emit to the atmosphere rather than to undergo further reduction to
N2. 相似文献
8.
Toshihiro NADE Kazuhisa FUJITA Masashi FUJII Masaya YOSHIDA Teikichi HARYU Satsuki MISUMI Toshiaki OKUMURA 《Animal Science Journal》2005,76(5):513-517
An X‐ray computed tomography (CT) system for live standing cattle was developed for studying the meat yield, carcass composition and so on. The gantry contained three X‐ray tubes and detectors that corresponded to each X‐ray tube. The system was able to operate while the animal remained standing. The scan area had a diameter of 900 mm. The Musculus longissimus and Musculus trapezius areas, and the back fat thickness in the CT image were evaluated and compared to the actual cross‐section of the carcass using eight cattle. The differences among the muscles, and the subcutaneous and intermuscular fat were easily recognized. The correlation coefficient between the CT image and the actual carcass photograph of the M. longissimus area and the back fat thickness was high (r = 0.84, r = 0.93, P < 0.01). The present study demonstrated that muscle, fat and bone can be clearly imaged from a live standing animal using X‐ray equipment. 相似文献
9.
lori INOUE Toshiaki OHARA Fumio NAMIKI Takashi TSUGE 《Journal of General Plant Pathology》2001,67(3):191-199
Restriction enzyme-mediated integration (REMI) mutagenesis was used to isolate mutants of Fusarium oxysporum f. sp. melonis impaired in pathogenicity. The race 2 strain Mel02010 was transformed with linearized pSH75, conferring resistance to hygromycin
B, with or without the enzyme used to linearize the plasmid. Addition of restriction enzymes did not affect the transformation
frequency. A total of 2929 REMI transformants were tested for pathogenicity to three melon cultivars, Amus, Ogon 9 and Ohi.
The race 2 strains are pathogenic to Amus and Ogon 9, but not to Ohi. Of 43 transformants with reduced pathogenicity on susceptible
melon cultivars, 12 mutants were examined in detail for pathogenicity, vegetative growth and integrative mode of pSH75. The
levels of pathogenicity varied among these mutants. Two mutants (B48 and B137) almost completely lost pathogenicity to both
susceptible cultivars, and the others had reduced pathogenicity. Mutants B48, B241, B886 and X36 were also impaired in vegetative
growth. Mutant B809 was a biotin auxotroph. By DNA gel blot analysis, nine mutants were found to contain a single copy of
the transformation vector. These mutants may thus be useful in isolating genes involved in pathogenicity.
Received 22 December 2000/ Accepted in revised form 16 April 2001 相似文献
10.
Sakai H Nakano H Yamaguchi R Yonemaru K Yanai T Masegi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):731-735
A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor. 相似文献