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Healthy yearling beef and dairy cattle were inoculated with a vaccine containing modified-live bovine respiratory syncytial virus (ML-BRSV), and sequential changes in clinical signs of disease, blood leukocyte subsets, BRSV-specific antibody titer, and in vitro lymphocyte blastogenic responses were monitored. Vaccination with ML-BRSV did not cause pyrexia, local or systemic hypersensitivity reaction, or respiratory tract disease. Episodes of leukopenia, abnormalities in lymphocyte subsets, or depression of phytomitogen-induced blastogenic responses were not observed subsequent to vaccination. Exposure to ML-BRSV resulted in at least a 16-fold increase in serum neutralizing antibody titer, with no increase seen in nonvaccinated contact controls. Significant BRSV-specific lymphocyte blastogenic responses were not detected, using one dose of several BRSV antigen preparations in a whole blood culturing system.  相似文献   
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Efficacy and safety of components of an IM-administered vaccine for prevention of infectious bovine rhinotracheitis virus (IBRV), parainfluenza type-3 (PI-3) virus, bovine viral diarrhea virus (BVDV), and respiratory syncytial virus (RSV) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. Challenge of immunity tests were conducted in calves for IBRV, PI-3 virus, or BVDV vaccinal components. All inoculated calves developed serum-neutralizing antibodies and had substantially greater protection (as measured by clinical rating systems) than did controls after challenge exposure to virulent strains of IBRV, PI-3 virus, BVDV, or RSV. In in utero tests, IBRV or bovine RSV vaccinal strains were inoculated into fetuses of pregnant cows. Histologic changes or abortions did not occur after fetal inoculation of the RSV vaccinal strain, and 10 of 14 fetuses responded serologically. Of 9 fetuses, one responded serologically to the IBRV vaccinal strain after in utero inoculation and was aborted 3 weeks later. In an immunologic interference test, 10 calves vaccinated with 2 doses of the multivalent vaccine, containing the 4 viral components and a Campylobacter-Leptospira bacterin, developed serum-neutralizing antibodies to IBRV, PI-3 virus, BVDV, and RSV without evidence of serologic interference. Under field conditions, 10,771 cattle, including 4,543 pregnant cows, were vaccinated. Vaccine-related abortions did not occur.  相似文献   
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Plant Foods for Human Nutrition - Circular use of resources implies developing mild processes to transform food by-products into value-added products, without using organic solvents or extensive...  相似文献   
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