甜菜坏死黄脉病毒(BNYVV)75kDa通读蛋白基因及其54kDa片段的克隆与表达

 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。     

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

压力实时监测法在管道泄漏检测中的应用

检测输油管道漏油的方法很多，介绍了通过压力实时监测确定输油管道漏油点方法，以及实际应用情况。实践证明，通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。     

一种快速分析香蕉果实采后mRNA水平变化的新方法

采用SMARTPCRcDNA合成与计算机软件相结合的方法,快速分析香蕉果实采后mRNA水平的变化。在28℃条件下分别将处于同株同穗同梳相邻位置的香蕉果实放置24、48、72h。应用这种方法对其总mRNA水平进行了分析,结果表明,香蕉采后48h时,与采后0h相比,mRNA水平最高。该方法由于使用了计算机软件TheScionImage,可对cDNA电泳图进行数字化分析,具有用材少,敏感性高,快捷方便等特点,特别适用于对较多样品进行分析。     

改进芳草A系原种产卵性的试验

本试验采取母蛾延迟交配，拆对后冷藏、改变交配时间和产卵室保护温度等技术措施来探讨改善芳草A系原种产卵性的方法。结果表明，这些措施对产卵性能的改进有一定的效应，但由于性状间存在相关关系，对某一性状的促进效应往往同时也对另一产卵性状产生负向影响。     

红虹木业，“顶端优势”之我见

《国际木业》：上海红虹木业有限公司作为一家拥有悠久历史的家族企业，自从事木材行业至今已发展为在北美、欧洲、东南亚等地区都有力量分布的规模供应商，整个过程中企业对自己的理念和战略有怎样的发展？     

深基坑锻锤基础冬期施工

在冬期施工，对于土方开挖、钢筋绑扎、人员的通行、砼的浇铸、砼的温度控制，基坑的保温等都带来一定困难，为此提出了深基坑锻锤基础才是最佳实践应用。     

HUDACAPP— 一种工艺规程自动设计系统

本文介绍浦沅工程机械总厂准柔性制造系统(P-FMS)中加工箱体零件所用的一个工艺规程自动设计系统—HUDACAPP,阐述了系统的原理、结构和实现.给出了实例.本系统能按零件输入信息自动生成工艺过程,选择机床、夹具、刀具、量具、切削参数以及计算工时,并能与P-FMS的实时管理调度软件联接,提供所需的资料.     

黑杨无性系日CO2固定总量的测定及与苗木生产潜势的相关性研究

通过对不同基因型黑杨无性系植株净光合速率日变化曲线与光合主体叶面积在时间和光强上进行累计积分来计算植株日CO2固定总量P。由于净光合速率日变化曲线不易准确测得，故以光响应曲线和光强的日变化来推测净光合速率日变化。对P值和生物量的相关性研究表明，二者的相关性很高。且认为可以用计算出的P值作为评估苗木生产潜势的指标之一，这为育种中生产力的早期选择提供了有力的证据。     

康绿功臣植物源农药防治苹果李始叶螨试验

以1．1％苦参碱康绿功臣粉剂、25％三氯杀螨醇乳油及73％克螨特乳油为供试药剂，在喀什垦区对苹果李始叶螨进行了小区及示范防治试验，结果表明，康绿功臣防治李始叶螨田间药效较好，具有安全、高效稳定、持效期长等优点，可以取代25％三氯杀螨醇。