Methane emission from irrigated rice ecosystem: relationship with carbon fixation,partitioning and soil carbon storage

Rice is a major agricultural crop and accounts for 40 % of the total food grain production of India. A field experiment was conducted for two successive seasons (December–June, 2012–13 and December–June, 2013–14) to assess the efficiency of rice varieties for methane (CH₄) emission in relation to atmospheric carbon fixation, partitioning of carbon, and storage in the soil. Six high yielding rice varieties, Bahadur, Cauvery, Dinanath, Joymoti, Kanaklata, and Swarnabh were grown under irrigated condition. Results of the present investigation depicted differences in photosynthetic rate among the varieties accompanied by differential ability for plant biomass partitioning between the shoots and the roots. Stomatal frequency of flag leaf at panicle initiation stage was found to have strong influence on photosynthesis. Low CH₄-emitting rice varieties, Bahadur and Dinanath, were found to have lower size of the xylem vessels than the high CH₄-emitting rice varieties, Joymoti and Kanaklata, and found to influence the CH₄ flux. Soil organic carbon storage of 0.505 Mg C ha^?1 y^?1 in the plough layer of soil (0–15 cm) confirmed that irrigated rice ecosystem is an effective sink of carbon. These findings suggest that selection of suitable rice varieties with higher photosynthetic efficiency and lower emission of CH₄ can be a suitable biological mitigation of this greenhouse gas. Although an inverse relationship of CH₄ with carbon dioxide (CO₂) efflux was observed, irrigated rice ecosystem has a good potential to store substantial amount of carbon in the soil.     

Identification of serum protein markers for early diagnosis of pregnancy in buffalo

Improper or delayed pregnancy diagnosis has significant impact over animal production, particularly in buffaloes which inherently suffer from several reproductive inefficiencies. Thus the present study has undertaken to identify serum protein markers pertaining to early pregnancy diagnosis in buffaloes. Serum samples were collected from 10 pregnant Murrah Buffalo heifers at weekly intervals from days 0‐35 post‐artificial insemination and from 12 inseminated non‐pregnant cyclic buffalo heifers on days 0, 7, 14 and 21. Two‐dimensional gel electrophoresis and densitometric analysis revealed the presence of five protein spots showing average density fold change of ≥4 during early pregnancy. Mass spectrometry analysis identified these up‐regulated proteins as anti‐testosterone antibody light chain, apolipoprotein A‐II precursor, serum amyloid A, cytokeratin type II, component IV isoform 1, which are have established roles in embryogenesis, but over‐expression of the fifth identified protein immunoglobulin lambda light chain in pregnancy has been elucidated as a novel finding in the current study. Further, with bioinformatics analysis, potential antigenic B‐cell epitopes were predicted for all these five proteins. An antibody cocktail‐based approach involving antibodies against all these five up‐regulated entire proteins or their epitopes could be developed for early detection of pregnancy in buffaloes. © 2016 Japanese Society of Animal Science     

Investigation into the Effect of Altitude on the Differential Hemocyte Count of Circulating Plasmatocytes and Granulocytes of Larval Stage of Antheraea assama (Lepidoptera: Saturniidae)

Differential hemocyte count of circulating plasmatocytes (PL) and granulocytes (GR) of fifth-instar larvae of Muga Silkworm Antheraea assama Westwood (Lepidoptera: Saturniidae) reared at four different sericulture farms situated at different altitudes, viz, Khanapara State Sericulture Farm, Assam, altitude 55.5 m above sea level (ASL); Nongpoh (Central Silk Board farm), Meghalaya, altitude 464 m ASL; Tura (Central Silk Board farm), West Garo Hills, Meghalaya, 657 m ASL; and Kalimpong (Central Silk Board farm), West Bengal, altitude 1,247 m ASL, were calculated and compared to investigate the effect of altitude on the number of PL and GR per mm³ of larval hemolymph. The investigation showed that the mean circulating PL and GR were highest at Khanapara (55.5 m ASL) located at the lowest altitude, whereas their numbers gradually decreased with increase in altitude at Nongpoh (464 m ASL), Tura (657 m ASL), and Kalimpong (1,247 m ASL). This may be attributed to the average environmental temperatures observed at different altitudes, which may affect the overall hemocyte load of larval stages reared at those altitudes.