Evaluation of antioxidant status and oxidative stress in cattle naturally infected with Theileria annulata

To assess the antioxidant status and oxidative stress in bovine theileriosis due to Theileria annulata blood samples were collected from 35 clinically affected cattle referred to Veterinary Teaching Hospital, School of Veterinary Medicine, Urmia University, Urmia, Iran. Complete blood count, piroplasm parasitemia percentage, erythrocyte glutathione peroxidase, superoxide dismutase, catalase and glucose-6-phosphate dehydrogenase activities, malondialdehyde concentration, osmotic fragility test and median corpuscular fragility were determined and the results were compared with those of 50 healthy controls. Of 35 affected cattle, 12 (34.28%) had severe anemia and 23 had mild to moderate anemia and parasitemia varied from 5 to 40%. The activities of erythrocyte glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase were significantly lower (P<0.0001) and the activity of catalase was significantly higher in the affected cattle than in healthy ones (P<0.001). Malondialdehyde concentration in erythrocytes of affected cattle was significantly more than those of healthy cattle (P<0.001). The affected cattle showed increased fragility of erythrocytes, so that median corpuscular fragility (MCF) in affected group was significantly lower than those of healthy group (P<0.0001). Median corpuscular fragility showed a positive correlation with the severity of parasitemia (r=0.81, P<0.0005) and a negative correlation with the activities of GSH-Px (r=-0.78, P<0.0001), SOD (r=-0.71, P<0.0005), catalase (r=-0.53, P<0.018) and G6PD (r=-0.58, P<0.0005). The results of this study suggest that oxidative damage to RBCs may contribute to the pathogenesis of anemia in bovine theileriosis.     

Assessment of serum thyroid hormone concentrations in lambs with selenium deficiency myopathy

OBJECTIVE: To assess changes in serum concentrations of thyroid hormones associated with selenium deficiency myopathy in lambs. ANIMALS: 35 lambs with selenium deficiency myopathy and 30 healthy lambs. PROCEDURES: Blood samples were collected via jugular venipuncture from lambs with selenium deficiency myopathy and healthy lambs. Activities of markers of selenium deficiency myopathy (erythrocyte glutathione peroxidase [GSH-Px] and plasma creatine kinase [CK]) and serum thyroid-stimulating hormone (TSH) and total thyroxine (tT(4)) and total triiodothyronine (tT(3)) concentrations were assessed; values in affected lambs were compared with those in healthy lambs. Correlations of erythrocyte GSH-Px and plasma CK activities with serum concentrations of TSH, tT(4), and tT(3) were investigated, and the tT(3):tT(4) concentration ratio was evaluated. RESULTS: Compared with findings in healthy lambs, erythrocyte GSH-Px activity, serum tT(3) concentration, and tT(3):tT(4) concentration ratio were significantly decreased and serum concentrations of tT(4) and TSH and the activity of plasma CK were significantly increased in affected lambs. Analysis revealed a significant negative correlation in the affected group between erythrocyte GSH-Px activity and each of the following: plasma CK activity (r = -0.443), serum TSH concentration (r = -0.599), serum tT(4) concentration (r = -0.577), and serum tT(3) concentration (r = -0.621). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that notable changes in circulating amounts of thyroid hormones develop in association with selenium deficiency in lambs. Such alterations in thyroid hormone metabolism may be involved in the high incidence of disorders, such as stillbirths and neonatal deaths, in selenium-deficient flocks.     

Nutritive value of sorghum silage for sheep

This study compared ensiled sorghum (ES) from two varieties (first and second cuts) with corn silage (CS) for chemical analysis, total-tract nutrient digestibility, ruminal variables and blood biochemistry metabolites in sheep. Five experimental silages representing first cut ES var. Pegah (PS1), second cut ES var. Pegah (PS2), first cut ES var. Speedfeed (SS1), second cut ES var. Speedfeed (SS2) and CS were assessed for chemical analysis, silage fermentation and anti-quality compounds. Diets consisted of lucerne hay, silage and a premix (at the ratio of 53.5:46:0.5 on a dry matter [DM] basis). Feed consumption, total-tract nutrient digestibility, microbial nitrogen supply (MNS), ruminal variables and blood biochemistry metabolites were determined in a Latin square design of five periods with five male sheep. Compared to sorghum, CS had lower (p < 0.01) concentrations of ash, water-soluble carbohydrates and crude protein, but higher starch, lignin (sa), non-structural carbohydrates and apparent nutrient digestibility. The measurements of pH, lactic acid, NH₃-N and volatile fatty acids (VFA) confirmed that all silages were well-preserved. The anti-quality compound levels in the silages were below the safe limits for ruminants. No differences were observed in feed consumption (p > 0.05) and total-tract digestibility of DM and nutrients (p > 0.05). Rumen pH, NH₃-N, VFA concentrations and acetate to propionate ratio showed no difference among treatments (p > 0.05). Sheep fed on sorghum silage showed a decrease (p < 0.01) in total protozoa, and particularly in the Entodiniae population. There were no differences in cellulolytic bacteria counts (p > 0.05) among treatments. Urinary N, MNS and blood biochemistry metabolites showed no difference among treatments (p > 0.05). Silages from both cuts of sorghum had comparable total-tract nutrient digestibility to CS, therefore, these varieties could be used as a more drought resilient silage source in the feeding of sheep.     

Influence of storage and drying methods on invertebrate elemental and isotopic measurements

Optimised pre-analytical methods for measuring the chemical properties of soil macro-organisms are needed. We tested the effects of ethanol immersion, freezer storage, and drying method on the reliability of estimates of key stoichiometric elements (carbon [C], nitrogen [N], phosphorus [P]) and abundances of ¹³C and ¹⁵N in samples of crickets (Acheta domestica), cockroaches (Nauphoeta cinerea) and mealworms (larval Tenebrio molitor). Ethanol immersion tended to increase A. domestica C and N, and reduced A. domestica P, relative to the reference treatment (deep freezing and oven drying). For N and P these effects were only present after 28-day ethanol immersion. Nauphoeta cinerea and T. molitor samples were generally unaffected by storage treatments, while δ¹³C and δ¹⁵N were not affected by any storage treatment for any species. Thus, five days of ethanol immersion may be acceptable prior to elemental and stoichiometric analyses of hard-bodied soil invertebrates in comparative studies.     

Wide hybridization and introgression breeding in safflower: Effectiveness of different selection methods

Possibility of using interspecific hybridization to increase genetic diversity of drought tolerance in safflower (Carthamus tinctorius L.) and effectiveness of different selection methods is poorly understood. The main objectives of this study were to evaluate the effectiveness of (a) interspecific hybridization to expand genetic diversity in safflower for agronomic traits and drought tolerance in advance generations and (b) different selection methods (direct vs. indirect) for improving seed yield. Interspecific hybridizations were performed between species of C. tinctorius × C. palaestinus (TP), C. palaestinus × C. oxyacantha (PO) and C. tinctorius × C. oxyacantha (TO). Based on the field screening of genotypes in F3 generation, four groups of selected genotypes (derived from combination of direct and indirect selection under both drought and normal environments along with selection based on drought tolerance index (STI)) were evaluated in F4 and F5 generations. Indirect selection for seed yield was significantly more efficient than direct selection. Selection in stress environment resulted to more efficiency than selection in normal environment. No significant difference was observed between direct selection and selection based on STI. The result showed that TP was the best population to combine high seed yield and drought tolerance in safflower.     

Soil quality assessment in semiarid rangeland in Iran

Abstract. We examined the use of soil quality (SQ) assessment to predict soil productivity and stability as a component of site potential for rangelands. Two minimum sets of data were compared for the SQ assessment within an area of relatively uniform climate. Data set 1 consisted of total soil N, topsoil depth, effective profile depth (EPD), and grade of structure, thus incorporating only soil chemical and physical properties. Data set 2 included exchangeable soil potassium, EPD, soil water retention capacity at wilting point, a soil slake test, and a nutrient cycling index. The interrelationships between soil properties and plant growth characteristics (i.e. total and herbage yield) were investigated and interpreted by statistical analysis and expert knowledge. By performing multiple regressions for each data set against the plant growth characteristics, we identified the contribution of each data set variable to the variability in plant characteristics and, thus, the predictive potential of each variable and data set. Within data set 1, EPD was important and in data set 2 the nutrient cycling index, which is a landscape function index derived from soil surface attributes, played the most important role in predicting potential. Principal component analysis was used to provide weighting factors for each indicator. We then transformed and combined observed indicator values for each data set using weighting factors and scoring functions into an additive soil quality index (SQI) varying in value from 0 to 1. The SQIs, with values greater than 0.8, provide optimum conditions for high yield.     

Zinc sorption–desorption by sand,silt and clay fractions in calcareous soils of Iran

Zinc sorption–desorption by sand, silt and clay fractions of six representative calcareous soils of Iran were measured. Sand, silt and clay particles were fractionated after dispersion of soils with an ultrasonic probe. Zinc sorption analysis was performed by adding eight rates of Zn from 6 to 120 μmol g^?1. For the desorption experiment, samples retained after the measurement of Zn sorption were resuspended sequentially in 0.01 M NaNO₃ solution and shaken for 24 h. Results indicated that Zn sorption by soil fractions increased in the order clay > silt > sand, and correlated negatively with CaCO₃ content and positively with cation exchange capacity (CEC) and smectite content. Results indicated that for all fractions, the Langmuir equation described the sorption rates fairly well. In contrast to sorption, Zn desorption from soil fractions increased in the order sand > silt > clay, and correlated positively with CaCO₃ content, CEC and smectite content. Results showed that parabolic diffusion and two constant equations adequately described the reaction rates of Zn desorption. In general, for all soils studied, the coarser the particle size, the less Zn sorption and more Zn desorption, and this reflects much higher risk of Zn leaching into groundwater or plant uptake in contaminated soils.     

Supercritical fluid extraction of alkylamides from Echinacea angustifolia

Echinacea has been known for its immunostimulatory activity, and its alkylamide components have been linked to such biological activity. Consequently, alkylamides in Echinacea angustifolia were extracted using supercritical carbon dioxide from fresh and dried roots at 45-60 degrees C and 34-55 MPa, and the alkylamide yield in the extracts was determined. The yield of alkylamides from fresh roots increased with temperature yet decreased with pressure, whereas the yield from air-dried roots (moisture content 8.4%) increased with both temperature and pressure. Freeze-drying of the roots to a moisture content of 4.9% did not result in any further increase in the yield compared to that of air-dried roots. Alkylamide yield of the ground dried roots extract was the highest (p < or = 0.05) among those from fresh, ground and unground E. angustifolia roots. Supercritical fluid extraction therefore shows potential for the recovery of alkylamides from dried Echinacea roots.     

Digital assessments of soil organic carbon storage using digital maps provided by static and dynamic environmental covariates

Understanding the vertical and lateral distribution of soil organic carbon (SOC) and soil organic carbon density (SOCD) is indispensable for soil use and environmental management because of their vital role in soil quality assessments. Primarily, they are needed in calculating soil organic carbon storage (SOCS). The objective of this research was to provide digital maps of SOC and SOCD variation as well as their uncertainties at multiple standardized depths (H1: 0–5, H2: 5–15, H3: 15–30, H4: 30–60 and H5: 60–100 cm) using a parsimonious model with optimized terrain-related attributes and satellite-derived data. SOCS were evaluated at soil subgroup levels. An area of about 808 km² with varying elevation, plant cover and lithology from the Miandoab region, West Azerbaijan Province, Iran was selected as a case study area. A total of 386 soil samples were collected from 104 profiles comprising various soil genetic horizons. A continuous spline function was then fitted to the target properties in advance of creating a dataset at five standard depth intervals (following the GlobalSoilMap project). These were then grouped into three classes including top (H1), middle (H2, H3 and H4) and bottom (H5) depths to ease interpretation. Static and dynamic covariates (30-m resolution) were derived from a digital elevation model (DEM) and a suite of Landsat-8 spectral imageries, respectively. Four candidate models including stepwise multiple linear regression (SMLR), random forest (RF), cubist (CU) and extreme gradient boosting (XGBoost) Tree were tested in this study. Finally, the digital maps at 30-m resolution of SOC and SOCD and their uncertainties were prepared using the best-fit model and the bootstrapping method, respectively. Four soil subgroups (Gypsic Haploxerepts, Typic Calcixerepts, Typic Haploxerepts and Xeric Haplocalcids) were identified across the study area. The covariates had variable contributions on the evaluated models. The XGBoost Tree model generally outperformed other models for prediction of SOC and SOCD (R² = 0.60, on average). Regardless of soil subgroups, the uncertainty analysis showed that the SOCD map had a low prediction interval range value indicating high accuracy. Additionally, the highest SOCS and SOCD was observed at the top followed by middle and bottom depths in the study area. All subgroups exhibited a decreasing trend of SOCD with increasing depth. A similar trend was also observed for SOCS. The highest SOCD (on average) was observed in Gypsic Haploxerepts (4.71 kg C/m²) followed by Typic Calcixerepts (4.46 kg C/m²), Typic Haploxerepts (4.45 kg C/m²) and Xeric Haplocalcids (4.40 kg C/m²). Overall, the SOCS normalized by area within soil order boundaries was greater in Inceptisols than Aridisols across the study area. The findings of this study provide critical information for sustainable management of soil resources in the area for agricultural production and environmental health in the Miandoab region of Iran.