Effect of harvest time on yield and pre-harvest quality of semi-leafless grain peas (Pisum sativum L.) as whole-crop forage

The aim of the work was to study changes in the yield and nutritional characteristics of whole crop semi-leafless field pea over two growing seasons in the Po plain, Italy. Samples of two cultivars (Baccara and Sidney) were collected from flowering to grain maturity. The developmental stage, yield, dry matter (DM) content, crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL), starch, water soluble carbohydrates (WSC), gross energy (GE), organic matter digestibility (OMD) and the net energy for lactation (NE_L) were determined at each harvest. The forage characteristics were regressed on the growing degree days (GDD) with 4.4 °C as the base temperature. The DM yield increased with advancing maturity from 0.5 to 8.91 Mg ha⁻¹, while the CP decreased from 261 to 159 g kg⁻¹ DM. During the whole growth cycle the GE, OMD, NE_L and milk forage units (milk FU) were almost steady. No differences were observed between the cultivars for any of the measured parameters. At grain maturity, the crop produced over 4.0 Mg ha⁻¹ DM of grain. The CP, starch and WSC of the grain did not show any differences between the cultivars or years. The data showed that the nutritive quality of the forage of the semi-leafless grain pea harvested as a whole crop for ensiling purposes did not diminish with maturity and could help improve the self-sufficiency of dairy farms, in terms of home-grown protein forages.     

Identification of Polyphenolic Compounds in Edible Wild Fruits Grown in the North-West of Italy by Means of HPLC-DAD-ESI HRMS

Plant Foods for Human Nutrition - The popularity of edible wild fruits has increased in industrialized countries due to their composition and positive effects. The aim of this study has been to...     

Effects of Dietary Protein Source and Feeding Regime on Growth Performance,Nutrient Digestibility,Fatty Acids,and Quality Characteristics of Rainbow Trout,Oncorhynchus mykiss,Fillets

This study investigated the effects of dietary protein source and feeding regime (apparent satiation and rationed) on growth performance, nutrient digestibility, fatty acid (FA) profile, and fillet quality traits in rainbow trout. A stock of 1200 juvenile trout (mean weight 114.6 ± 0.2 g) were randomly distributed into 24 fiberglass tanks (four diets × three replications × two feeding regimes). The experimental diets were formulated to be isoproteic and isoenergetic based on bacterial protein meal (BPM), pea protein concentrate (PPC), mixture thereof (MIX), and fish meal (FM), respectively. The feeding trial lasted 77 d with water temperature of 13 C. Statistical differences appeared among the diets only in terms of crude protein digestibility. Growth performance and somatic indexes were significantly affected by the diet, while only the condition factor was influenced by the feeding regime. None of the parameters appeared to be affected by the interaction effects. Differences appeared between the FA profiles of the dorsal muscle. Oleic, linoleic, α‐linolenic, and docosahexaenoic acid contents were influenced by diet, while only minor FAs were influenced by feeding regime. In conclusion, growth performance and nutrient digestibility resulted lower in fish fed BPM diet than other groups, while PPC group was similar to FM group.     

Intake and Apparent Digestibility of Permanent Meadow Hay and Haylage in Ponies

The aim of this article was to study the conservation quality, chemical composition, gross energy, voluntary intake, and apparent digestibility of permanent meadow haylage and hay, fed to ponies at maintenance. The forages were from the same swath and harvested at early flowering stage. The apparent digestibility of the hay and haylage, which contained about 61% neutral detergent fiber and 38% acid detergent fiber, as fed, was determined by means of two in vivo digestibility trials, each performed on six ponies, weighing, on average, about 335 ± 80 kg and 334 ± 41 kg, respectively, over a 6-day feces collection period after a previous 14-day adaptation period. The voluntary intake was measured and the digestibility coefficients were calculated for dry matter, organic matter, gross energy, crude protein, neutral detergent fiber, and acid detergent fiber, using acid-insoluble ash as the internal marker. The haylages were evaluated for dry matter, pH, alcohols, lactic acid, and monocarboxylic acid. The conservation quality of the haylage was good and its chemical composition was similar to that of hay, except for the crude protein and gross energy contents, which were higher (P < .05) as compared with those of the hay. No differences were found between the hay and haylage with regard to the voluntary intake and digestibility coefficients.     

Rice protein concentrate meal as potential dietary ingredient in practical diets for blackspot seabream (Pagellus bogaraveo)

The aim of this study was to evaluate the performances and the flesh quality of Pagellus bogaraveo fed with diets containing rice protein concentrate [RPC, 70% crude protein (CP) and 10% ether extract]. Three isoproteic and isoenergetic (CP 47%, 22 MJ/kg DM) diets were formulated with an increasing level of RPC: 0%, 20% and 35%. The fish (mean weight 75 g) from the Messina Straits were randomly distributed in 12 tanks (3 diets x 4 replications, 10 fish/tank). The daily ratio (1.5% of the fish biomass) was updated every 15 days. Biomass gain showed an opposite trend to the RPC diet inclusion. No differences appeared in the somatic indexes. Differences appeared between fatty acid profiles of the dorsal muscle. Fatty acid of series n-6 increased and fatty acid of series n-3 decreased in fillets of fish fed with increasing levels of RPC. The inclusion of RPC in the diets, as a partial replacement of fish meal (20%), is possible without affecting the growth performance and fillet quality.     

Lactobacillus rhamnosus as additive for maize and sorghum ensiling

The effects of Lactobacillus rhamnosus AT195, a potential probiotic microorganism cultured in buffalo "scotta" whey, on chemical and microbiological composition in maize and sorghum ensiling were evaluated. Both crops were harvested, chopped, and treated or not with the selected strain prior to ensiling in fiberglass vertical silos; 90 days after ensiling, silages were sensorially evaluated and sampled. Different chemical components were evaluated both on fresh crops and silages: in particular, the water-soluble carbohydrates content was investigated by high-field NMR spectroscopy and the carbohydrate fermentation profile was performed by GC. Besides phenotypic identification and typing, microbiological studies included Lb. rhamnosus genotype typing by RAPD-PCR. All silages, inoculated or not, were well preserved, as their chemical and microbiological data along with the fermentation profiles showed. The selected strain used as inoculum influenced the lactic acid population of silages and evidenced a good survival performance during the ensiling process of both maize and sorghum. Moreover, the use of Lb. rhamnosus strain efficiently improved the quality of the multifactorial ensiling process by significantly reducing the ammonia nitrogen content of both maize and sorghum silages.     

Use of different levels of false flax (Camelina sativa L.) seed in diets for fattening rabbits

An experiment has been conducted to study the effects of various levels of false flax (Camelina sativa L.) seed (FFS) in the diet on the growth performance, some carcass characteristics and fatty acid profile of rabbits meat and fat. In the experiment, a total of 30 weaned crossbred rabbits aged 70 days and weighing, on average, 2316 g were equally divided into three groups of 10 (five male and five female rabbits each). Three levels (0%, 10%, or 15%) of FFS were included in isonitrogenous and isocaloric diets. The experimental period lasted 50 days. At the end of the experiment, there were no significant differences among the groups in live weight, live weight gain, feed consumption, feed efficiency, carcass yield and the percentages of edible organs. The percentage values of head, skin and limbs, fore legs, hind legs, breast and ribs, loin and abdominal wall were not affected by the inclusion level of FFS. Although the chemical composition of the meat was not significantly affected by the dietary treatment, the polyunsaturated fatty acid (PUFA) concentration in the longissimus dorsi muscle and perirenal fat was significantly increased with the increasing of FFS inclusion, while saturated fatty acid (SFA) decreased. The n−6/n−3 PUFA ratio of the meat decreased from 3.86 in the control group, to 1.19 in the 15% of FFS group. These results showed that the use of a diet supplemented with FFS was effective in reducing the saturation, atherogenic and thrombogenic indexes, with consequent benefits on the nutritional quality of rabbit meat for consumers, without significant adverse effects on growth performance and carcass characteristics.     

Effects of Spirulina and plant oil on the growth and lipid traits of white sturgeon (Acipenser transmontanus) fingerlings

The aim of this research was to evaluate the efficiency of diets with Spirulina and plant oils (POs) inclusion for white sturgeon weaning and their effects on the fatty acid (FA) composition of fish flesh. Three isoproteic (45%) and isoenergetic (21 MJ kg^?1 DM) diets were formulated: one fish meal‐based diet integrated with fish oil (FMO) and two 40% Spirulina meal‐based diets integrated with corn (SPC) or soybean (SPS) oils respectively. One hundred and thirty‐five white sturgeon fingerlings (mean weight 17.5 g) were stocked randomly in nine fibreglass tanks. At the end of the trial, which lasted 71 days, the growth performance traits and somatic indexes were determined. The chemical composition, gross energy and FA profile were determined on the fish fillets. No significant effects were observed for the growth performances or fillet chemical composition. The FA profile of the fillets reflected those of the diets. In particular, the fillets of the fish fed with the SPC and SPS diets were lower in n‐3 FA, due to the substitution of fish oil (FO) with POs. It is possible to replace FO and meal in sturgeon; therefore, Spirulina meal integrated with POs could be a good alternative to sturgeon diet.     

Intake and apparent digestibility of hay or hay plus concentrate diets determined in horses by the total collection of feces and n-alkanes as internal markers

The apparent digestibility of hays plus two commercial mixed feeds containing about 14% and 11.8% crude fiber (CF), respectively as feeds was determined by means of two in vivo digestibility trials, each performed on 4 or 5 saddle horses weighing on average about 550 and 535 kg, respectively, over a 6 day feces total collection period with a previous 14 day adaptation period. The diets were based on a first cut meadow hay – whose digestibility was estimated in each trial – and different percentages of the mixed feeds at a feeding level close to maintenance. The two mixed feeds were rich respectively in a by-product from the citrus fruit industry (citrus pulp) and in a by-product from the apple fruit industry (apple pectin pulp). The forage to concentrate ratio was, respectively, 50:50, 75:25 and 100:0 on a dry matter basis (Diets 1, 2 and 3) in the first trial and, 50:50 and 100:0 (Diets 4 and 5) in the second trial.

The apparent digestibility of the dry matter, organic matter, gross energy, and crude protein was measured by the ingesta/excreta procedure and using the most abundant n-alkanes (C₂₇, C₂₉, and C₃₁) as internal markers for all the rations and the hays.

No differences were found among the digestibility coefficients obtained by the total collection method or using the different n-alkanes.

The use of n-alkanes for digestibility determinations in horses is neither precise nor accurate, but can be advised for free ranging animals, when the total collection of feces is impossible. Taking into account that the accuracy of this later method is comparable, we suggest selecting n-alkanes with a high concentration and the highest fecal recovery; C₂₉ and C₃₁ n-alkanes (the first in particular) resulted to be the best choice.