Effect of storage duration on egg quality,embryo mortality and hatchability in FUNAAB-ɑ chickens

Effect of extended storage on egg quality, embryo mortality and hatchability in FUNAAB-ɑ chickens was determined. Hatchable eggs (n = 288; weighing 53.2 ± 4.67 g) collected from a flock of FUNAAB-ɑ layer breeder hens aged 32 weeks were stored in egg tray with broad end up under 16 ± 1.5°C for either 0, 4, 8, 12, 16 or 20 d. Before incubation, eight eggs from each group were evaluated for internal and external quality traits. Remaining eggs were set in an incubator and transferred into hatcher on embryonic day 18. Data collected were subjected to one-way analysis of variance. Egg weight loss (EWL; p < .001), surface area (p < .001), yolk diameter (p < .001), inner and outer blastoderm diameters (p < .05) and dead in germ (DIG; p < .001) increased with storage duration while yolk height (p < .001), yolk index (p < .001), albumen weight (p < .05), albumen height (p < .05), albumen index (p < .01), Haugh's unit (HU; p < .05), fertility (p < .001), hatchability of set (HATCHS; p < .001) and fertile eggs (p < .05) decreased. Weight losses of 0, 1.2, 2.2, 3.4, 4.6 and 6.1% were recorded in egg stored for 0, 4, 8, 12, 16 and 20 days respectively. Eggs stored beyond 8 days exhibited higher DIG and lower HATCHS. Shell percentage in 4 days storage (11.4%) was lower (p < .05) than in 16 days storage (13.4%). Shell thickness was similar in eggs stored for 0 to 12 days, but 8 days storage (0.60 mm) had thinner (p < .01) shell than day 16 (0.71 mm) and day 20 (0.73 mm) storage. Internal quality unit (IQU) was higher (p < .05) in fresh eggs (180.4) than in 12 days (167.8) and 20 days (167.8) stored eggs. Extended storage of FUNAAB-ɑ eggs caused EWL, surface area shrinkage, lowered HU and IQU, loss of yolk and albumen quality, increased blastoderm diameters and DIG, and decreased egg fertility and HATCHS from day 8 forward. Storing FUNAAB-ɑ eggs beyond 8 days reduced quality parameters; therefore, other mitigating factors are recommended when storing beyond 8 days.     

Prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria

Using a cross-sectional survey, we determined the prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria. Antibodies to Brucella species in serum samples were tested using the Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (cELISA); for milk, the milk ring test (MRT) and indirect-ELISA (i-ELISA) were used. Questionnaire was administered to cattle herdsmen to determine factors predisposing the animals to bovine brucellosis. Data were analyzed using STATA 12. From 513 serum and 635 milk samples tested among 120 herds, overall animal-level prevalence of 10.1% (95% CI 7.5–12.7%) and 20.2% (95% CI 17.1–23.3%) were recorded by RBT and MRT, respectively; while 9.4% (95% CI 6.9–11.9%) and 17.8% (95% CI 14.8–20.8%) were obtained using cELISA and i-ELISA, respectively. In all, from the 120 herds tested, 29.2% and 43.3% were positive by RBT and MRT, respectively. Multivariable logistic regression revealed that herd location (OR?=?8.12, 95% CI 1.68–38.90) and improper disposal of placenta/fetus (OR?=?17.33, 95% CI 4.81–62.33) were predictors for a seropositive herd using RBT; while herd location (OR?=?5.13, 95% CI 1.27–20.28), large herd size (OR?=?2.62, 95% CI 1.15–5.85), and occurrence of abortion for a year or more (OR?=?4.62, 95% CI 1.53–13.71) were predictors of seropositivity to antibodies to Brucella spp. using MRT. We found high prevalence of brucellosis in cattle herds under extensive management system in southwestern Nigeria. Urgent and coordinated control strategies are required to mitigate this problem.

     

Factors influencing the biochemical markers for predicting Mammalian oocyte quality

The need for accurate selection of the best oocytes for in vitro fertilization protocols and thus, production of embryos has driven the search for oocyte quality markers from morphological criteria to biochemical parameters. Current studies are focused on the biochemical constituents of the follicular fluid and gene expression profiling of the cumulus cells. These parameters are, however, affected by factors that must be considered before making a judgment of the oocyte's quality. These includes factors such as the type of hormonal stimulation protocol, age of oocyte donor and heat stress on the donor, all of which have been reported to influence the concentrations of many hormones, apolipoproteins, metabolites, fatty acids and growth factors in the follicular fluid and the expression of several genes in the cumulus cells. Another important point to note is species variation in the response to these extraneous influences, which thus calls for species targeted investigations. As reports are still scanty and investigations assumed to be very keen, we employed this review paper to bring attention of researchers and clinicians to those factors that may come to bear on the outcome of their investigations on oocyte and embryo quality.     

Physicochemical characteristics of flours of faba bean as influenced by processing methods

Wet- and dry-processing with and without heating treatments were used to dehull faba beans for preparation of flours from the cotyledons. Flour qualities were assessed by levels of tannin and trypsin inhibitor and other measures of proximate composition. High roasting temperature and shorter process time that improved the recovered cotyledon yields were verified significant by path analysis methodologies. In turn, the higher cotyledon recoveries correlated with higher protein levels and inversely with the measured tannin levels. Ash reductions were correlated to the wet processing options in hull removal while reductions in the insoluble dietary fiber were notably influenced by level of heating temperatures applied. Trypsin inhibitor levels ranged from 42 to 56% of the original with reductions tied to applications of wet and/or heat processing in each case improved by increased durations and temperatures of treatments.     

Chemical evaluation of waste from Thaumatococcus danielli (Benth) processing

Three different batches of Thaumatococcus danielli (Benth) fruits were collected at different fruiting seasons. The proximate compositions of the pericarp and seeds were determined on a dry weight basis. Partial characterization of the lipid extract was carried out and tannin content determined. The average moisture, ash and lipid contents of the pericarp were higher (89.2 ± 4.17%, 20.7 ± 1.10% and 11.6 ± 1.23%, respectively) than those of the seed (31.2 ± 4.66% 9.1 ± 0.71% and 8.2 ± 1.64%, respectively). Crude protein and total carbohydrate were, however, higher in the seed (9.5 ± 4.38% and 69.4 ± 11.52%, respectively) than in the pericarp (4.5 ± 2.21% and 6.3 ± 3.94%, respectively). The dietary fiber contents of both pericarp and seed were high. Starch content was very low in the pericarp (0.4 ± 0.0%) compared to the seed (66.28 ± 9.21%). Tannin content in the pericarp and seeds was 12.1 ± 1.52 mg/g and 21.9 ± 2.28 mg/g, respectively. The oil extracted from both pericarp and seeds was light-yellow in color and slightly solid at room temperature. Unsaturation level of T. danielli oil was low, while free fatty acids were high. The results obtained suggested that T. danielli waste could potentially be a raw material in livestock feed formulation.     

Effects of Sodium and Potassium Application on Water Content and Yield of Tomato in Southwestern Nigeria

The effects of sodium (Na) and potassium (K) application on tomato (Lycopersicon esculentum (L.) Mill) were studied in soil culture. The study was a 4 × 4 factorial, arranged into a randomized complete block design (RCBD) and replicated four times. Sodium was applied at 0, 5, 10, and 20 mg Na/kg soil as NaCl and K as KCl at 0, 20, 40, and 80 mg K/kg soil. Records of dry matter yields, water content, mineral nutrient, and number of fruits were taken. The application of 10 to 20 mg Na/kg soil and 80 mg K/kg soil depressed the water contents and the fruit yield. The application of 5 mg Na/kg soil and 40 mg K/kg soil produced the highest number of fruits, indicating a Na and K ratio of 1:8. A Na: K ratio of 0.45 up to 0.60 and 1.44 up to 1.80 for shoot and root, respectively, maintained good shoot water balance, resulted in lower floral abortion, and markedly enhanced the fruit yield. A complimentary relationship between Na and K was observed at Na: K ratios between 1:4 and 1:8.     

Optimizing herbicidal efficacy of glyphosate isopropylamine salt through ammonium sulphate as surfactant in oil palm (Elaeis guineensis) plantation in a rainforest area of Nigeria

A randomized complete block field trial was conducted over 2 years to evaluate the herbicidal efficacy of glyphosate isopropylamine salt (Roundup^®) applied singly at 1.41 kg a.e. ha⁻¹ or in combination with ammonium sulphate (AMS) as surfactant at 0.5, 1.0, 1.5, and 2.0% (wt/v) in a 5-year old oil palm plantation of the Federal University of Technology, Akure located in the rainforest vegetation zone of Nigeria. Assessment of herbicidal efficacy based on the Henderson-Tilton formula indicated that glyphosate applied singly or in combination with varying concentrations of AMS proved highly effective in controlling most of the grasses and broadleaved weeds prevalent in the experimental plots. Herbicidal efficacy on total weed density, weed fresh weight as well as on weed dry weight was found to increase in the order of increasing concentration of AMS. Regressing percentage herbicidal efficacy (Y) against increasing concentration levels of AMS (X) indicated significant (P0.001) positive relationships with an average correlation coefficient (r) of +0.95 in both years. These results confirm the potential of AMS as an effective surfactant that can be exploited in glyphosate-based weed management schemes in the sub-humid tropical environment.     

Effect of additive and carrier type on potato sprout suppression by dust formulations of tecnazene

Summary Tecnazene formulations, with kaolin and alumina as carriers respectively and with some containing Triton X-405 (15% w/w) or, Tween 80 (15% w/w) as additive, were applied on potatoes stored at 10°C and 15–18°C respectively. Tween 80 enhanced sprouting inhibition by alumina formulation while Triton X-405 reduced that with kaolin as carrier. Formulation with kaolin as carrier produced greater sprouting inhibition than that with alumina as carrier.