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Amin Bigham-Sadegh Iraj Karimi Mahsa Alebouye Zahra Shafie-Sarvestani Ahmad Oryan 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(3):337-343
The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 ± 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups. 相似文献
2.
Leila Behbood Soroush Karimi Esmaeil Mirzaei Ghobad Mohammadi Mahsa Azami Elham Arkan 《Fibers and Polymers》2018,19(7):1454-1462
The mucoadhesive Chitosan (CS) nanofibers as a drug delivery system were developed. Chitosan was modified via the immobilization of thiol groups from L-cysteine as a mucoadhesive reagent. The mucoadhesive properties of the chitosan nanofibers were evaluated by tensiometer set and via tensile studies. Drug and mucoadhesive agent loading lead to decrease diameters and increased porous of nanofibers. The release of Tetracycline (Tet) and Triamcinolone (Tri) were increased with increasing immersion time and it became constant at long immersion times. Mucoadhesion studies were done at pH 2–7 and in pH 6 maximum mucoadhesive properties observed. Release studies demonstrated a sustained release of both drug continued up to 48 hours. Microbial studies were performed on the nanofibers. The drug delivery system represented a novel tool for improve the therapeutic efficacy of various drugs that are poorly absorbed from the gastrointestinal tract. Also it is an efficient system for treatment of oral ulceration. 相似文献
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Bahareh Zadehmohseni Mohammad Zakeri Vahid Yavari Mahsa Haghi 《Aquaculture Research》2020,51(8):3443-3451
The effects of different salinity levels, including 10–15, 30–35, 70–75, 110–115 and 150–155 ppt, were evaluated on survival and the body amino acids (BAA) of Artemia franciscana. The results were expressed as total essential (TE), non‐essential (NE) and total amino acids (TAA); also, the ratio of the TE to NE (ENAA) on days 3 and 13 of the culture is reported. The study of changes in Artemia BAA showed that with the increase in the water salinity, the TE, NE and TAA increased significantly on days 3 and 13 of the culture. However, the highest ratio of ENAA was observed in the 110 gL?1 salinity treatment on day 3 (1.067 ± 1.25). Regarding the effects of different salinity treatments on the survival rate of Artemia, it was observed that, generally, an increase in the water salinity would reduce the survival rate of this species. This reduction was observed on day 3 of culture in the salinity treatments above 120 (66.66 ± 1.68%) and below 30 gL?1 (89.66 ± 0.34%) and on day 13 of culture in the salinity treatments below 30 (11.86 ± 0.13%) and above 70 gL?1. Basically, it can be concluded that A. franciscana culture at a salinity of 110 gL?1 can lead to the biological enrichment of Artemia in terms of amino acid profile. 相似文献
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Regulatory Effect of let-7f Transfection in Non-Small Cell Lung Cancer on its Candidate Target Genes
Venus Zafari Milad Asadi Nasim Bakhtiyari Mahsa Sadeghzadeh Majid Khalili Habib Zarredar Soghra Bornehdeli Ensiyeh Seyedrezazadeh 《Iranian Biomedical Journal》2022,26(3):209
Background:Let-7f has essential impacts on biological processes; however, its biological and molecular functions in lung cancer pathogenesis have yet been remained unclear. We aimed to investigate the expression level of let-7f and its candidate target genes both in lung cancer tissues and A549 cell line. Methods:Bioinformatics databases were first used to select candidate target genes of let-7f. Then the relative gene and protein expressions of let-7f and its target genes, including HMGA2, ARID3B, SMARCAD1, and FZD3, were measured in lung tissues of Non-Small Cell Lung Cancer (NSCLC) patients and A549 cell line using quantitative real-time PCR and Western blotting. The electroporation method was used to transfect A549 cells with let-7f mimic and microRNA inhibitor. The impact of let-7f transfection on the viability of A549 cells was assessed using MTT assay. The expression data of studied genes were analyzed statistically Results:Results indicated significant downregulated expression level of let-7f-5p (p = 0.0013) and upregulated level of the HMGA2 and FZD3 in NSCLC cases (p < 0.05). In A549 cells, after transfection with let-7f mimic, the expression of both mRNA and protein levels of HMGA2, ARID3B, SMARCAD1, and FZD3 decreased. Also, the overexpression of let-7f significantly inhibited the A549 cell proliferation and viability (p = 0.017). Conclusion:Our findings exhibited the high value of let-7f and HMGA2 as biomarkers for NSCLC. The let-7f, as a major tumor suppressor regulatory factor via direct targeting genes (e.g. HMGA2), inhibits lung cancer cell viability and proliferation and could serve as a marker for the early diagnostic of NSCLC.Key Words: MicroRNAs, Biomarkers, Carcinoma, Non-Small-Cell Lung 相似文献
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Jahangir Abedi-Koupai Jaleh Varshosaz Mahsa Mesforoosh Amir Hossein Khoshgoftarmanesh 《Journal of plant nutrition》2013,36(8):1130-1138
There have as yet been no detailed studies of micronutrient release from polymer coated fertilizers (PCFs). In this research, slow release microcapsules containing iron fertilizer was made and its release pattern evaluated. Three polymers ethyl cellulose (EC), glycerol mono stearate (GMS) and Compritol 888 ATO, were mixed with avicel and lactose and the microcaopsules were made by extrusion/spheronization method. The dissolution test carried out to determine the Fe release rate versus time. The results showed that, microcaopsules containing Compritol 888 ATO, released iron ions slower than that of EC and GMS. The release rate reached 85% after 7 days. In order to reduce the release rate, these microcaopsules were coated with ethylene vinyl acetate (EVA) polymer by dip coating method. It is concluded that the combination of matrix type formulation and the coating with EVA polymer caused a significant decrease in the release rate of iron. 相似文献
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Najmeh Zarei Hosnieh Ghasemi Mahsa Nayebhashemi Mozhgan Zahmatkesh Monire Jamalkhah Nafiseh Moeinian Zahra Mohammadi Somayeh Enayati Vahid Khalaj 《Iranian Biomedical Journal》2021,25(4):255
Background:The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant proteins, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which its deletion extends RLS. Herein, a los1-deficient strain of P. pastoris was generated and characterized. Methods:A gene disruption cassette was prepared and transformed into an anti-CD22-expressing strain of P. pastoris. A δ los1 mutant was isolated and confirmed. The drug sensitivity of the mutant was also assessed. The growth pattern and the level of anti-CD22 ScFv expression were compared between the parent and mutant strains.Results:The los1 homologue was found to be a non-essential gene in P. pastoris. Furthermore, the susceptibility of los1 deletion strain to protein synthesis inhibitors was altered. This strain showed an approximately 1.85-fold increase in the extracellular level of anti-CD22 scFv (p < 0.05). The maximum concentrations of total proteins secreted by δ los1 and parent strains were 125 mg/L and 68 mg/L, respectively.Conclusion:The presented data suggest that the targeted disruption of los1 homologue in P. pastoris can result in a higher expression level of our target protein. Findings of this study may improve the current strategies used in optimizing the productivity of recombinant P. pastoris strains. Key Words: Aging, Longevity, Pichia pastoris, Recombinant proteins 相似文献
7.
Hamid-Reza Fallahi Gholamreza Zamani Mokhadereh Mehrabani Mahsa Aghhavani-Shajari Alireza Samadzadeh 《Journal of Crop Science and Biotechnology》2016,19(1):77-84
Mother corm size is the most important factor for production of replacement corms, flower, and stigma yield in saffron. In semi-arid regions, water shortage is a deterrent agent for appropriate corm growth of saffron. Therefore, in this experiment the effects of six levels of superabsorbent polymer (SAP) including 0, 10, 20, 30, 40, and 50 kg ha-1 was investigated on growth indices of replacement corms of saffron. For this purpose, an experiment was evaluated at the research farm of the Saffron Research Group, Sarayan Faculty of Agriculture, University of Birjand, Iran, during 2013-2015. Results showed that SAP application did not considerable effect on corm growth indices in the first life cycle of saffron. Nevertheless, consumption of 30-40 kg ha-1 SAP somewhat improved the number and total weight of replacement corms per clone. In the second life cycle of saffron, the positive impact of SAP was clearly observed on the growth of replacement corms. The application of 40 kg ha-1 SAP increased the amount of number of replacement corms per clone by 13%, total weight of replacement corms per clone by 36%, and scale weight of corms per clone by 50% compared with control. In addition, the amount of mean weight of replacement corms and mean number of buds per corm in treatment of application of 40 kg ha-1 SAP were 29 and 27% higher than the control treatment, respectively. SAP application decreased the amount of non-standard (< 8 g) corm production while significantly increased the percentage of corms with standard weight. Overall, the increasing effect of SAP application on corm weight, bud number, and standard corm yield was higher than the number of produced corms per clone. In addition, water-use efficiency in SAP application treatments (6.1 kg standard corm m-3) was more than the control (4.34 kg m-3) treatment. In total, SAP application is an appropriate strategy for production of standard saffron corms in semi-arid regions. 相似文献
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Seyed Saeed Mirzargar Ali Taheri Mirghaed Seyyed Morteza Hoseini Melika Ghelichpour Mahsa shahbazi Morteza Yousefi 《Aquaculture Research》2022,53(1):191-198
The present study aimed at assessing the effects of addition of thymol [0 (T0), 2.5 mg/L (T2.5), 5 mg/L (T5) and 10 mg/L (T10)] to water on biochemical and antioxidant responses of common carp, Cyprinus carpio, during 3-h transportation. The lowest dissolved oxygen and ammonia levels after transportation were related to T2.5 and T5 treatments respectively. T5 treatment exhibited lower cortisol/glucose responses after transportation and 24 h after that, whereas T2.5 and T10 exhibited elevations in these parameters at the same times. Hyperammonaemia and hyperchloraemia were observed in T2.5 treatment immediately after transportation, but such changes were observed in all treatments, 24 h after transportation. Addition of 10 mg/L thymol to water prevented uraemia after transportation, but all thymol-treated fish exhibited uraemia, 24 h after transportation. Thymol treatment failed to mitigate lactate elevation immediately after transportation; nevertheless, T2.5 and T10 had, respectively, the lowest and highest lactate levels, 24 h after transportation. T5 treatment inhibited alteration in superoxide dismutase activity, immediately after transportation, and T10 exhibited a similar effect, 24 h after transportation. Addition of 2.5 mg/L thymol to water significantly increased catalase activity immediately after transportation, but such a response was observed in all treatments, 24 h after transportation. T2.5 and T10 exhibited higher malondialdehyde levels than T0 immediately after transportation, but such a response was observed in all thymol-treated fish, 24 h after transportation. In conclusion, addition of 5 mg/L thymol is beneficial in reducing water ammonia concentration and stress in carp during transportation. 相似文献