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排序方式: 共有205条查询结果,搜索用时 15 毫秒
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M E Kehrli K A Weigel A E Freeman J R Thurston D H Kelley 《Veterinary immunology and immunopathology》1991,27(4):303-319
Blood neutrophil functions, lymphocyte blastogenic responses, serum complement, and serum conglutinin activity of 98 lactating Holstein cows from two genetic lines were evaluated. The genetic lines were produced in a selection experiment that created and perpetuated genetic differences in milk production for up to seven generations. No significant differences between the two genetic lines of cows were found for neutrophil function, lymphocyte blastogenic responses, serum complement levels, or serum conglutinin levels. Significant differences between sire progeny groups within lines were found for unstimulated and mitogen-stimulated lymphocyte blastogenesis (P less than 0.0001), and almost all neutrophil functions (antibody independent neutrophil cytotoxicity, antibody dependent neutrophil cytotoxicity, ingestion of bacteria, iodination, chemiluminescence, chemokinesis, and chemotaxis (P less than or equal to 0.05)). Sire progeny group differences (P less than or equal to 0.0001) within lines for serum complement and conglutinin activity were also found. Neutrophil chemiluminescence activity (positive relationship; P less than or equal to 0.001), concanavalin A-stimulated lymphocyte blastogenesis (positive relationship; P less than or equal to 0.004), and serum conglutinin activity levels (negative relationship; P less than or equal to 0.01) each had small but significant associations with the total milk somatic cell count. Cows seropositive for bovine leukosis virus had increased resting and mitogen-stimulated lymphocyte blastogenic activity and were associated with increased in vitro neutrophil random migration and production of superoxide anion. Estimates of genetic parameters of various immune cell functions, of serum complement and of conglutinin levels for daughters of 11 sires with 4-6 daughters in the data set were determined. In this report, genetic variation was demonstrated for nonspecific humoral and cellular immunity. 相似文献
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Comparative,multidimensional imaging of patent ductus arteriosus and a proposed update to the morphology classification system for dogs
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Removal of hemangiosarcoma cells from canine blood with a cell salvage system and leukocyte reduction filter
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Kelley Zimmerman Koranda A. Walsh Jonathan T. Ferrari Nicholas S. Keuler Matthew J. Atherton Jennifer A. Lenz 《Veterinary and comparative oncology》2023,21(3):503-508
Multi-agent chemotherapy successfully induces remission in most naïve, high-grade canine lymphoma patients; however, disease recurrence is common. MOPP (mechlorethamine, vincristine, procarbazine, and prednisone) is an effective rescue protocol used to re-induce remission, but is associated with gastrointestinal toxicity and can be a less desirable option for patients that previously failed vincristine-containing protocols. Therefore, alternative members of the vinca alkaloid family, such as vinblastine, could be potentially advantageous as substitutes for vincristine to reduce gastrointestinal toxicity and chemoresistance. The objective of this study was to report the clinical outcomes and toxicity of 36 dogs with relapsed or refractory multicentric lymphoma treated with a modified MOPP protocol whereby vincristine was replaced with vinblastine (MVPP). The overall response rate to MVPP was 25% with a median progression free survival of 15 days and a median overall survival of 45 days. MVPP at the prescribed doses resulted in modest and transient clinical benefit, but was well tolerated with no treatment delays or hospitalizations secondary to side effects. Given the minimal toxicity, dose intensification could be considered to improve clinical responses. 相似文献
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Xin-Tao He Frank J. Stevenson Richard L. Mulvaney Kenneth R. Kelley 《Soil biology & biochemistry》1988,20(6):857-862
Studies were conducted on an 15N-enriched Illinois Mollisol (Flanagan silt loam) to determine the extractability of newly immobilized N with aqueous phenol (45%, v/v). In comparison to mild extradants proposed as indexes of plant available N (i.e. 10 mw NaHCO3 and hot 10 m
CaCl2), phenol showed the highest selectivity for the immobilized N, both with regard to the amount of biomass N extracted and the magnitude of the extractability ratio (ER). Aqueous phenol was also more selective in removing the immobilized N than reagents used for extraction of soil organic matter (i.e. 150 m
Na4P2O7 and 100 m
KOH). Results with soils incubated for up to 120 days showed that the amount of 15N extracted with aqueous phenol fluctuated with time and was related to changes in the soil microbial population, e.g. ER was highest when the bacterial population was maximal. Aqueous phenol shows promise as a selective extradant of potentially-available (labile) organic N in soils. 相似文献
10.
Complementary DNA sequencing: expressed sequence tags and human genome project 总被引:227,自引:0,他引:227
M D Adams J M Kelley J D Gocayne M Dubnick M H Polymeropoulos H Xiao C R Merril A Wu B Olde R F Moreno 《Science (New York, N.Y.)》1991,252(5013):1651-1656
Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields. 相似文献