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Chin Soon Lionel Ng Tai Chong Toh Kok Ben Toh James Guest Loke Ming Chou 《Aquaculture Research》2014,45(11):1852-1860
The dietary habits of the sea urchin Salmacis sphaeroides and the gastropod Trochus maculatus in ex situ mariculture were examined. Fouling algal assemblages on terracotta tiles were exposed to the grazers and compared. Except for Day 0, the assemblages on Days 2, 13 and 27 differed significantly across treatments. S. sphaeroides rapidly consumed most algae, leaving an assemblage dominated by turf algae and bare tile. T. maculatus primarily fed on green filamentous algae, resulting in an even distribution of other algal functional groups, while control tiles were dominated by green filamentous algae. Using three representative fouling algae species (Bryopsis corymbosa, Hypnea spinella and Lobophora variegata), the consumption rates of both grazers and dietary preferences of S. sphaeroides were examined through a single‐diet and a choice experiment respectively. The single‐diet experiment revealed differential algal consumption rates for S. sphaeroides (H. spinella = B. corymbosa > L. variegata) and T. maculatus (B. corymbosa > H. spinella = L. variegata). The choice experiment showed that S. sphaeroides preferred H. spinella over the other two species. These experiments highlighted the importance of understanding the dietary habits of grazers as this has direct implications on their effectiveness as biological controls of fouling macroalgae in mariculture. 相似文献
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Shyu DJ Chou WM Yiu TJ Lin CP Tzen JT 《Journal of agricultural and food chemistry》2004,52(5):1350-1356
A cDNA fragment encoding cystatin, a cysteine protease inhibitor, was obtained from maturing sesame seeds. The clone was constructed in a nonfusion or fusion vector and then overexpressed in Escherichia coli. The recombinant cystatins were found in the soluble fraction of cell extract and were demonstrated to be functionally active in a reverse zymographic assay. The corresponding endogenous 22 kDa cystatin of low abundance in mature seeds was purified to homogeneity via a papain-coupling affinity column and confirmed by western blotting with antibodies against the recombinant cystatin. Both endogenous and recombinant cystatin proteins showed effective inhibitory activities against papain with K(i) values of 7.89 x 10(-8) M and 2.77 x 10(-8) M, respectively. Immunodetection indicated that cystatin was specifically expressed in maturing seeds and rapidly degraded in germination. Accordingly, zymographic and inhibition analyses showed that sesame cystatin could not inhibit the de novo synthesized proteases in germinating seeds. It is suggested that sesame cystatin may play a role in the regulation of endogenous cysteine proteases during seed maturation and germination. 相似文献
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半干旱黄土丘陵区典型抗旱造林整地技术集流效果研究 总被引:4,自引:0,他引:4
以宁南山区生态建设中推广应用的典型抗旱造林整地工程为研究对象,针对工程措施的集流效果进行研究,结果表明:实施"88542"集流造林整地工程,能够有效地改善土壤的水分状况,尤其在0-60 cm土层表现较为明显;工程措施的集流效果随地形的变化有所不同:随坡位自上而下改变,"88542"集流水平沟比集流坡面水分的增加率总体上呈增大趋势;随着坡长增大,工程措施对水分增加率也呈增大趋势;随坡度的增大,水平沟相对集流面的水分增加率也随之增加;随坡向不同,工程措施的集流效果则无明显差异;相对于工程措施的集流效果,坡长和坡度之间呈正相关关系,故在工程设计中,坡度越大,相应集流坡面长度就要缩短,反之坡度越缓,集流坡面长度就要增加. 相似文献
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红皮云杉嫩枝扦插繁殖技术的研究 总被引:9,自引:0,他引:9
本文描述了红皮云杉嫩枝扦插繁殖技术。通过试验筛选出1种适合于红皮云杉嫩枝扦插的基质(插壤),2种使生根率达98%且根系发育良好的生根剂。同时,提出了把握扦播时机,合理运用生根剂浓度及处理时间的方法和技术措施。 相似文献
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