Preharvest applications of growth regulators and their effect on postharvest quality of table grapes during cold storage

Over 54,600 ha of table grapes (Vitis vinifera), mainly cvs. ‘Thompson Seedless’, ‘Flame Seedless’ and ‘Redglobe’, are planted in Chile. Almost the entire production is exported to the USA, Europe, Asia, or one of several Latin American countries, which typically requires 15–40 d of maritime transportation. During this period, several physical, physiological, and pathological factors cause table grape deterioration. Because berry size is the main quality factor in international markets, farmers often overuse the growth regulators, gibberellic acid (GA₃) and forchlorfenuron (CPPU), in an effort to increase berry size. We examined the effect of preharvest growth regulators on seedless (‘Thompson Seedless’, and ‘Ruby Seedless’) and seeded (‘Redglobe’) table grape cultivars during cold (0 °C) storage plus a shelf life period of 3 d at 20 °C. The overuse of GA₃, eight instead of two GA₃ applications on Thompson Seedless, and the use of one GA₃ application on Redglobe and ‘Ruby Seedless’, increased berry pedicel thickness and lowered cuticle content but induced shatter and predisposed grapes to gray mold caused by Botrytis cinerea. In contrast, CPPU increased berry pedicel thickness and cuticle content but did not increase shatter or gray mold incidence. Clusters that were subjected to overuse of combined GA₃ and CPPU were highly sensitive to shatter, had the thickest pedicel, and developed a high gray mold incidence during cold storage. Hairline, a fine cracking developed during cold storage, was induced on ‘Thompson Seedless’ and ‘Ruby Seedless’ by growth regulators, but no hairline occurred on ‘Redglobe’ table grapes. Therefore, berry quality during cold storage is greatly influenced by growth regulator management in the vineyard.     

结球莴苣“烧边”成因及其调控措施的研究进展

结球莴苣烧边病是生理性病害，严重影响其品质，制约生产。国内外多数研究者认为钙缺乏导致结球莴苣烧边病的发生，但是现在许多研究表明其他因素也能导致烧边病的出现，例如环境因素、生长速率、内源激素等，所以研究烧边病发生的机制及其与环境因素的关系是防御结球莴苣烧边病发生的关键。     

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

TGF-β信号通路在鱼类性别决定与分化中的作用

脊椎动物性别分化和性腺发育的分子机制保守,但不同类群的最上游的性别决定基因却大不相同,尤其是鱼类,其性别决定基因表现出明显的多样性。性别决定包括环境性别决定和遗传性别决定,环境性别决定主要受温度、光照、激素和pH等的影响,而遗传性别决定一般由位于性染色体上的性别决定基因决定。转化生长因子-β(transforming growth factorβ, TGF-β)信号通路参与介导了多种生物学过程,近年来很多研究表明,鱼类有多个性别决定基因都是TGF-β信号通路的成员,且该信号通路对于鱼类的性别分化也有重要的作用。本文总结了鱼类已报道的性别决定基因或候选基因,详细综述了TGF-β信号通路在鱼类性别决定与分化中的各种功能,并探讨了该信号通路参与鱼类性别决定的可能机制,这对认识TGF-β信号通路在鱼类性别决定、分化中的作用和性控育种有重要意义。     

脑部特异表达rtTA调控元件转基因小鼠的构建

为获得一种可调控基因表达系统,避免传统转基因在胚胎发育早期表达的毒性作用或致死作用以及基因过度表达而造成难于分析的复杂多表型,应用分子生物学方法将四环素反式激活子rtTA克隆到仅在脑部能被激活的钙调蛋白依赖性蛋白激酶CaM kinase Ⅱα亚基启动子之后,将其通过显微注射方法注入FVB小鼠受精卵雄原核中获得四环素调控系统中反应组分的转基因工具鼠.经PCR及Southern检测,最终得到3只阳性小鼠(1公2母),表达检测结果显示,其中仅2只(1公1母)后代检测到rtTA表达产物.结果表明已成功构建四环素调控系统中的转基因工具鼠,为今后研究其他脑部基因功能提供便利.     

不同贮存温度和时间对蜂王浆中游离氨基酸的影响

将新鲜蜂王浆分别贮藏在不同时间和温度下,对比分析游离氨基酸的含量及组成。结果测得蜂王浆中含有21种游离氨基酸,以脯氨酸含量最高。在室温下贮藏时,苏氨酸变化最大;-20℃时,丝氨酸变化最大;甲硫氨酸和组氨酸在-20℃下较稳定,含量几乎不变,在室温下15d内分别下降12．5％和17．4％,30d后分别下降37．5％和40．0％。由此我们可以推测甲硫氨酸和组氨酸在一定程度上可以代表蜂王浆的新鲜度。     

农村老龄化下风险偏好、人际关系与农户化学品施用行为分析

基于441份农户的微观调查样本,从各类化肥使用量、农药使用量及其购买成本方面考察了农村劳动力老龄化对化学品投入的影响,并从老年劳动力的体力状况、控制感、技能需求、风险态度和人际关系等途径探究其影响机制,进一步考察农村老年劳动力个体特征对化学品投入产生的异质性影响。结果表明,农村劳动力老龄化会显著减少对复合肥的使用量以及化肥的购买成本。当控制了体力和技能需求等变量后,老龄化对化学品投入依旧有显著的负向影响,表明以往研究推测的体力和技术采纳是老龄化影响务农的途径,不能全面揭示其影响机制。进一步研究发现,农村劳动力老龄化会影响农户的人际关系以及风险偏好对务农投入的边际效应。结论表明,中国建设生态宜居的美丽乡村政策对国家粮食安全战略具有促进作用。     

生草和树枝覆盖对果园土壤持水性能的影响

针对黄土高原枣园普遍盛行传统清耕制,将生草及覆盖技术引入枣园生产中,于2011—2013年采用人工土槽模拟研究方法,探讨不同生草和覆盖措施对枣树地土壤持水性能的影响。结果表明:生草与枣树枝覆盖能有效改善土壤物理结构、提高土壤孔隙度、降低土壤容重;各处理土壤水分蓄持能力及比水容量均按枣树枝半覆盖+白三叶生草、枣树枝全覆盖、白三叶生草覆盖和清耕处理依次递减,处理间的差异在高吸力阶段更为明显;与清耕处理相比,生草与覆盖处理土壤饱和含水量、田间持水量、凋萎系数均有提高,枣树枝半覆盖+白三叶生草处理增加最为明显,分别增加9%、20%、33%。     

玉米秸秆切割过程的非线性数值模拟与仿真试验

应用Solidworks软件建立玉米秸秆—切割刀片参数化模型,借助LS-DYNA971非线性有限元软件模拟切割刀片切割玉米秸秆的过程。玉米秸秆切割的有限元数值模拟能降低试验成本和缩短试验时间,可视化数值模拟过程可为玉米秸秆收获机械的设计提供理论参考。利用参数化模型特点,通过改变玉米秸秆直径大小、秸秆含水率、切割方式和刀片刀端线速度进行正交仿真试验。结果表明：对切割力来说,含水率对其影响最显著,其它影响依次为：秸秆直径、切割方式、刀片刀端线速度;对切割功耗来说,含水率影响最显著,其次分别为：秸秆直径、刀片刀端线速度、切割方式。经正交综合平衡法分析可知：当刀片刀端线速度为28 m·s^-1,采取滑切方式,玉米秸秆含水率、直径分别为45%、26 mm时为最优。玉米秸秆—切割刀片动力学可视化数值模拟仿真模型的构建和分析, 对玉米秸秆收获机械设计具有重要的参考价值。     

阿维菌素和硫线磷对南方根结线虫的毒力

利用阿维菌素和硫线磷处理南方根结线虫离体卵与幼虫,测试其对线虫卵和卵块孵化以及对2龄幼虫的致死作用、活动能力、侵入能力的影响。结果表明阿维菌素和硫线磷均对线虫卵和卵块的孵化表现很强的抑制作用,阿维菌素5 μg/mL处理24 h对卵孵化抑制率高达82.57%,而硫线磷40 μg/mL处理24 h对卵孵化抑制率为73.05%;阿维菌素和硫线磷处理48 h对2龄幼虫的LD50分别为7.346 1、13.345 6 μg/mL;在阿维菌素2 μg/mL和硫线磷3 μg/mL处理中,2龄幼虫在沙柱中的平均移动距离仅为0.04 cm和0.05 cm,而对照处理的线虫平均移动距离为1.04 cm;在阿维菌素2 μg/mL和硫线磷50 μg/mL处理2龄幼虫48 h后,对番茄根部的侵入率为0.12%和0.45%,而空白对照处理线虫的侵入率达21.85%。