首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   2448篇
  免费   123篇
  国内免费   116篇
林业   195篇
农学   237篇
基础科学   68篇
  325篇
综合类   700篇
农作物   235篇
水产渔业   157篇
畜牧兽医   615篇
园艺   51篇
植物保护   104篇
  2023年   17篇
  2022年   50篇
  2021年   92篇
  2020年   53篇
  2019年   49篇
  2018年   53篇
  2017年   53篇
  2016年   56篇
  2015年   64篇
  2014年   79篇
  2013年   104篇
  2012年   156篇
  2011年   178篇
  2010年   103篇
  2009年   109篇
  2008年   168篇
  2007年   163篇
  2006年   157篇
  2005年   128篇
  2004年   80篇
  2003年   79篇
  2002年   82篇
  2001年   69篇
  2000年   69篇
  1999年   49篇
  1998年   50篇
  1997年   36篇
  1996年   34篇
  1995年   28篇
  1994年   26篇
  1993年   40篇
  1992年   22篇
  1991年   17篇
  1990年   27篇
  1989年   12篇
  1988年   25篇
  1987年   14篇
  1986年   9篇
  1985年   13篇
  1984年   8篇
  1983年   10篇
  1979年   6篇
  1978年   5篇
  1977年   3篇
  1976年   3篇
  1975年   4篇
  1974年   6篇
  1973年   5篇
  1972年   3篇
  1970年   3篇
排序方式: 共有2687条查询结果,搜索用时 15 毫秒
1.
2.
将红檬实生苗培养于改良无土基质中,接种泡囊-丛枝菌根Glomuscitrocolum,以不接种菌根为对照,并分别施用含氮量相等的3种氮肥,即硝酸按(NO3-N)、尿素(NH2-N)和硫酸铵(NH4-N)。接种菌根16周后,施不同氮肥的红檬实生苗均有效地感染了菌根,并促进了植株的生长。试验证明,红实生苗施硝态氮(NO3-N)使植株的菌根依赖性有明显提高。  相似文献   
3.
Eastern equine encephalitis (EEE) was diagnosed in a flock of emus in southeastern Louisiana. The outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. The diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of EEE antibody in two recovered emus. High mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. On postmortem examination, hemorrhagic enteritis and multiple petechia of viscera were observed. Microscopic changes included severe necrosis of hepatocytes, intestinal mucosa, and necrotizing vasculitis of the spleen and lamina propria of the intestine. No nervous system lesions were observed. This outbreak occurred concurrently with EEE in horses and was attributed to unseasonably heavy rainfall with an abundance of arthropod vectors and proximity to free-living reservoir host species.  相似文献   
4.
5.
Significant efforts should be devoted to reducing waste outputs from aquaculture operations in order to lower the environmental impacts of aquaculture in many parts of the world. Since most aquaculture wastes are ultimately from dietary origin, reduction of waste outputs should first be through improvements of diet formulation and feeding strategies. The first step in the production of feeds producing less solid waste is to eliminate poorly digestible ingredients (such as whole grain or grain by-products used as binders and fillers in the feed formulae) and to use highly digestible ingredients with good binding properties. Further reduction of solid waste can then be achieved through careful selection of the ingredients to improve apparent digestibility and the nutrient balance of the feed. Nitrogen waste outputs can be reduced through the reduction of the digestible protein to digestible energy (DP/DE) ratio of the diet. Phosphorus waste outputs can be reduced through careful selection of the ingredients and optimization of the digestible phosphorus content of the diet to meet the requirement of the fish but avoid greatly exceeding this required level. Finally, feeding practices that minimize feed wastage should be adopted since feed wastage can have a very significant impact on waste outputs from fish culture operations.  相似文献   
6.
Since 1993, 14 cases of avian influenza from four different states in the U.S.A. have been diagnosed by virus isolation from eight avian species. Only 11 of the 14 avian influenza virus (AIV) primary isolations would have been successful if only the standard protocol for AIV isolation, i.e., inoculation of specific-pathogen-free embryonating chicken eggs (ECEs) by the chorioallantoic sac (CAS) route, had been followed. Primary isolation attempts were negative for AIV in three cases in which ECEs were inoculated by the CAS route; AIV could not be detected by hemagglutinating activity, agar gel immunodiffusion test or negative stain electron microscopy. However, in these three cases, primary isolations of AIV were achieved by inoculation of ECEs into either the yolk sac or onto the chorioallantoic membrane.  相似文献   
7.
The recommended therapeutic doses of three kinds of tetracyclines, oxytetracycline (OTC, withdrawal period, 10 days), chlortetracycline (CTC, withdrawal period, 5 days) and tetracycline (TC, withdrawal period, 5 days), were each administered to a group of 15 pigs. Blood was sampled before drug administration and during the withdrawal period. The concentration of tetracyclines in plasma, determined by semi-quantitative ELISA, was compared with that of internal standard (10 ppb as oxytetracycline). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine the tissue residues in pigs. All 45 plasma samples from nontreated pigs showed negative in the residue of any of three tetracycline antibiotics. OTC was detected in plasma of pigs treated until the 8th day, CTC until the 4th day, and TC was detected until the 3rd day of its withdrawal period. The present study showed that the semi-quantitative ELISA easily be adopted in predicting tissue residues for tetracycline antibiotics in live pigs.  相似文献   
8.
This article establishes for the export industroes the index system of the assessment and disscusses the steps for using DEA model. In addition it also sets up a relative assessment model for the eport industries. Practice showsthe model is more satisfact  相似文献   
9.
The structural gene for glutathione S-transferase in Oryza sativa was successfully cloned from a cDNA library by the polymerase chain reaction method. The deduced amino acid sequence of this gene showed 44-66% similarity to the sequences of the class phi GSTs from Arabidopsis thaliana and Zea mays. This gene was expressed in Escherichia coli with the pET vector system and the gene product was purified to homogeneity by GSH-Sepharose affinity column chromatography. The expressed OsGSTF3-3 was a homo-dimer composed of 24 kDa subunit and its pI value was approximately 7.3. The OsGSTF3-3 was retained on GSH affinity column and its Km value for GSH was 0.28 mM. The OsGSTF3-3 displayed high activity toward 1-chloro-2,4-dinitrobenzene, a general GST substrate and also had high activities towards acetanilide herbicides, alachlor, and metolachlor. The OsGSTF3-3 was highly sensitive to inhibition by benastatin A and S-hexyl-GSH. From these results, the expressed OsGSTF3-3 is a phi class GST and seems to play an important role in the conjugation of the chloroacetanilide herbicides.  相似文献   
10.
The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号