左旋咪唑在生长育肥猪驱虫中的应用研究

本文探讨了5%左旋咪唑注射液按10mg/kg 体重剂量的不同驱虫次数对生长育肥猪驱虫效率、中毒反应及生产性能的影响。结果表明,1)在60～240日龄全期试验内,分别于60和150日龄各驱虫1次的试验Ⅰ组猪日增重极显著高出对照组(不驱虫)的23.9%(p<0.01);也显著高于只在60日龄驱虫1次的试验Ⅱ组(p<0.05),尽管Ⅱ组猪日增重显著高于对照组14.5%(p<0.05)。2)用药后,猪只应激毒性反应为100%,但均能自然康复。3)2次驱虫的效率比1次驱虫的高,且彻底。因此,用左旋咪唑给生长育肥猪驱虫,宜进行2次(分别于60和150日龄),可显著改善生长性能和提高经济效益,适宜在农(山)区养猪中推广。     

类风湿关节炎的蜂疗医治法

类风湿关节炎多为对称性，发病原因不明，多发于20-45岁，女性多于男性。现代医学认为此病与感染、自身免疫下降、遗传因素、内分泌失调等有关，而寒冷和潮湿是诱发本病的重要因素。     

试论福建省国营林场分类管理

本文分析了福建省110个国营林场的类型、目前管理体制、管理办法及存在的问题。指出了目前国营林场管理办法中存在着种种弊端,跟深化林业改革很不适应,必须改革和完善国营林场管理机制,理顺“省办地管县监督”的管理体制,实行分类指导。本文就分类管理的依据,原则和实施办法进行了探讨,并提出了一些建议。     

Studies on the chiral isomers of fonofos and fonofos oxon: II. In vitro metabolism

The in vitro metabolism of the chiral isomers of fonofos and fonofos oxon in the presence of mouse liver mixed-function oxidase and serum esterase was investigated. The metabolism of ³⁵S-labeled phenyl-(S)_P-fonofos mediated by mixed-function oxidase took place stereoselectively, resulting predominantly in (R)_P-fonofos oxon. Similarly, (R)_P-fonofos was converted to (S)_P-oxon. In each case, however, a significant amount of racemization occurred. Other products were diphenyl disulfide and diphenyl disulfide oxide. In addition to stereospecificity, the oxidative metabolism of (R)_P-fonofos proceeded at a rate faster than that of (S)_P-fonofos. Stereoselective rate differences also were observed in mouse or rat serum-catalzyed degradation of the fonofos oxon enantiomers, the (S)_P isomer being degraded about twofold faster than its enantiomer. The differences in toxicities of the isomers of fonofos and fonofos oxon were consistent with the in vitro metabolism data.     

钙及其拮抗剂对苹果果肉质膜透性的调节作用

采用培养果肉圆片的方法,研究了Ca2+及其拮抗剂对苹果果肉质膜透性的调节作用。结果表明,CaCl2(1、10mmol/L)降低果肉膜透性和溶质外渗速率(Js);细胞膜Ca2+通道阻塞剂Verapamil(100μmol/L)的影响不显著;细胞外Ca2+螯合剂EGTA(5mmol/L)、CaM的拮抗剂CPZ、TFP(100μmol/L)明显提高果肉膜透性和细胞溶质外渗速率。培养24h时,CaCl2能明显维持较高的SOD活性和ACC向乙烯的转化能力,EGTA、Verapamail、CPZ和TFP的作用相反。这些说明Ca2+对果肉细胞膜具有保护作用,而减少细胞外Ca2+和抑制细胞内Ca2+-CaM功能对果肉细胞膜具有伤害作用。     

Isolation and Characterization of Efficient Active Compounds Using High-Performance Centrifugal Partition Chromatography (CPC) from Anti-Inflammatory Activity Fraction of Ecklonia maxima in South Africa

Ecklonia maxima is a brown seaweed, which is abundantly distributed in South Africa. This study investigated an efficient approach using high-performance centrifugal partition chromatography (HPCPC), which has been successfully developed for the isolation and purification of phlorotannins, eckmaxol, and dieckol from the ethyl acetate fraction of E. maxima (EEM). We evaluated EEM for its inhibitory effect against lipopolysaccharide (LPS)-induced inflammatory responses in zebrafish embryos. The separation of eckmaxol and dieckol from samples of EEM using HPCPC was found to be of high purity and yield under an optimal solvent system composed of n-hexane:ethyl acetate:methanol:water (2:7:3:7, v/v/v/v). To evaluate the anti-inflammatory efficacy of EEM containing active compounds, zebrafish embryos exposed to LPS were compared with and without EEM treatment for nitric oxide (NO) production, reactive oxygen species (ROS) generation, and cell death two days after fertilization. These evaluations indicate that EEM alleviated inflammation by inhibiting cell death, ROS, and NO generation induced by LPS treatment. According to these results, eckmaxol and dieckol isolated from brown seaweed E. maxima could be considered effective anti-inflammatory agents as pharmaceutical and functional food ingredients.