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排序方式: 共有70条查询结果,搜索用时 15 毫秒
1.
In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network 下载免费PDF全文
AH Alm‐Kristiansen ER Gaustad G Bai FB Standerholen G Klinkenberg E Kommisrud KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370
Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital® (SV) technology, the first commercialized production line of SpermVital® (C) and by conventional procedure applying Biladyl® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen. 相似文献
2.
Vikingstad E Andersson E Norberg B Mayer I Klenke U Zohar Y Stefansson SO Taranger GL 《Fish physiology and biochemistry》2008,34(3):289-298
Atlantic salmon (Salmo salar L.) females (2 SW), maturing for the first time, were reared under one of three temperature regimes (high: 14.3 ± 0.5°C;
natural: 10.6 ± 1.0°C; and cold: 6.9 ± 1.0°C) in combination with one of two experimental treatments; an injection of GnRH
analogue (GnRHa) contained in biodegradable microspheres, or a sham injection (microspheres only). The six experimental groups
were then reared under simulated natural photoperiod for 4 weeks. Blood samples were drawn for analysis of plasma steroid
levels and the fish were inspected for ovulation weekly. Batches of stripped eggs were incubated in triplicate incubators
in raceways until the eyed stage. Treatment with GnRHa resulted in a substantial advancement and synchronization of ovulation
at all temperatures, while exposure to cold water also appeared to advance ovulation slightly. While 75% (warm and cold) to
90% (natural) of GnRHa fish ovulated during the 4-week trial, only 30% of sham-treated females exposed to cold water, and
none of the sham-treated fish held at higher temperatures, ovulated during this period. Survival rates of embryos to the eyed-stage
were significantly higher for broodstock exposed to cold water. Plasma levels of testosterone (T), 17β-oestradiol (E2), and 17α,20β-dihydroxy-4-pregnen-3-one (17,20βP) were all significantly affected by treatment with GnRHa and, to a lesser
extent, temperature. The efficiency of GnRHa in counteracting the negative effects of high temperature on ovulation and the
associated changes in circulating sex steroids suggest that temperature inhibition operates at least in part at the brain
or pituitary. 相似文献
3.
Ecological effects of climate fluctuations 总被引:3,自引:0,他引:3
Stenseth NC Mysterud A Ottersen G Hurrell JW Chan KS Lima M 《Science (New York, N.Y.)》2002,297(5585):1292-1296
Climate influences a variety of ecological processes. These effects operate through local weather parameters such as temperature, wind, rain, snow, and ocean currents, as well as interactions among these. In the temperate zone, local variations in weather are often coupled over large geographic areas through the transient behavior of atmospheric planetary-scale waves. These variations drive temporally and spatially averaged exchanges of heat, momentum, and water vapor that ultimately determine growth, recruitment, and migration patterns. Recently, there have been several studies of the impact of large-scale climatic forcing on ecological systems. We review how two of the best-known climate phenomena-the North Atlantic Oscillation and the El Ni?o-Southern Oscillation-affect ecological patterns and processes in both marine and terrestrial systems. 相似文献
4.
Eva Andersson Sampath R. Kumar Jared Ackers Geir Lasse Taranger Sigurd Olav Stefansson John M. Trant 《Fish physiology and biochemistry》2003,28(1-4):411-411
Photoperiod and temperature modulated the seasonal pattern of ovarian gene expression of P450 aromatase, Follicle-Stimulating Hormone receptor and Luteinizing Hormone receptor in Atlantic salmon broodstock. 相似文献
5.
J. W. Seinhorst Mej C. H. Klinkenberg F. A. v. d. Meer 《European journal of plant pathology / European Foundation for Plant Pathology》1956,62(1):5-6
Summary Raspberries and strawbereis planted several years after a previous crop of raspberries were found attacked byPratylenchus penetrans. Raspberries planted in places where appled trees had been taken out showed slightly stunted growth and a moderate attack of the roots byP. penetrans.The symptoms of heavily infested raspberries were: severely stunted growth of above ground parts without other particular symptoms; a very poor root system, parts of which are completely dead and blackened or show dead, blackened bark, and the absence of fine feeding roots. In cases of light attack the root system is more normal but there is a number of rather large black lesions.Raspberries should not be planted after raspberries, after fruit trees susceptible to attack byP. penetrans, after strawberries or on possibly infested soil (in general light soils) unless a determination of the degree of infestation in the plant sites has shown that the place is safe. In case of infestation suitable measures to eradicate the nematodes should be taken. It may be expected that a fairly light degree of infestation of the soil is sufficient to cause appreciable damage. 相似文献
6.
Halbmayr E Mathiesen G Nguyen TH Maischberger T Peterbauer CK Eijsink VG Haltrich D 《Journal of agricultural and food chemistry》2008,56(12):4710-4719
This work presents the cloning and expression of the genes encoding heterodimeric beta-galactosidases from Lactobacillus reuteri L103, Lactobacillus acidophilus R22, Lactobacillus plantarum WCFS1, and Lactobacillus sakei Lb790. These enzymes consist of two subunits of approximately 73 and 35 kDa, which are encoded by two overlapping genes, lacL and lacM, respectively. We have cloned these genes into the lactobacillal expression vectors pSIP403 and pSIP409, which are based on the sakacin P operon of L. sakei ( S?rvig et al. Microbiology 2005, 151, 2439- 2449 ), and expressed them in the host strains L. plantarum WCFS1 and L. sakei Lb790. Results varied considerably, ranging from 2.23 to 61.1 U/mg of beta-galactosidase activity, depending on the origin of the lacLM genes, the host strain, and the expression vector used. Highest expression levels were obtained in a laboratory cultivation of L. plantarum WCFS1 harboring the plasmid pEH3R containing the lacLM gene from L. reuteri L103. These cultivations yielded approximately 23 000 U of beta-galactosidase activity per liter, corresponding to the formation of roughly 100 mg of recombinant protein per liter of fermentation medium, and beta-galactosidase levels amounted to 55% of the total intracellular protein of the host organism. To further verify the suitability of this expression system, recombinant beta-galactosidase from L. reuteri was purified to apparent homogeneity. The properties of the purified enzyme were essentially identical with the properties of purified native beta-galactosidase from L. reuteri L103. The presented results lead the way to efficient overproduction of beta-galactosidase in a food-grade expression system, which is of high interest for applications in food industry. 相似文献
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9.
The increasing need to account for the many factors that influence fish population dynamics, particularly those external to the population, has led to repeated calls for an ecosystem approach to fisheries management (EAFM). Yet systematically and clearly addressing these factors, and hence implementing EAFM, has suffered from a lack of clear operational guidance. Here, we propose 13 main factors (shift in location, migration route or timing, overfishing (three types), decrease in physiology, increase in predation, increase in competition, decrease in prey availability, increase in disease or parasites and a decline in habitat quality or habitat quantity) that can negatively influence fish populations via mechanisms readily observable in ~20 population features. Using these features as part of a diagnostic framework, we develop flow charts that link probable mechanism(s) underlying population change to the most judicious management actions. We then apply the framework for example case studies that have well‐known and documented population dynamics. To our knowledge, this is the first attempt to provide a clearly defined matrix of all the probable responses to the most common factors influencing fish populations, and to examine possible diagnostics simultaneously, comparatively and relatively in an attempt to elucidate the most probable mechanisms responsible. The framework we propose aims to operationalize EAFM, thereby not only better diagnosing factors influencing fish populations, but also suggesting the most appropriate management interventions, and ultimately leading to improved fisheries. We assert the framework proposed should result in both better use of limited analytical and observational resources and more tailored and effective management actions. 相似文献
10.
Nguyen TT Mathiesen G Fredriksen L Kittl R Nguyen TH Eijsink VG Haltrich D Peterbauer CK 《Journal of agricultural and food chemistry》2011,59(10):5617-5624
Food-grade gene expression systems for lactic acid bacteria are useful for applications in the food industry. We describe a new food-grade host/vector system for Lactobacillus plantarum based on pSIP expression vectors and the use of the homologous alanine racemase gene (alr) as selection marker. A new series of expression vectors were constructed by exchanging the erythromycin resistance gene (erm) in pSIP vectors by the L. plantarum WCFS1 alr gene. The vectors were applied for the overexpression of β-galactosidase genes from L. reuteri L103 and L. plantarum WCFS1 in an alr deletion mutant of L. plantarum WCFS1. The expression levels obtained in this way, i.e. without the use of antibiotics, were comparable to the levels obtained with the conventional system based on selection for erythromycin resistance. The new system is suitable for the production of ingredients and additives for the food industry. 相似文献