High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.     

抓具抓取轨迹曲线及其绘制

在设计和研究抓具系统的功能和各种特性时,首先必须对抓具颚爪的抓取曲线进行研究。本文分析和论述了抓具的抓取轨迹曲线、影响抓取轨迹曲线的因素以及抓取曲线的绘制。     

Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.     

柠檬酸废液深层培养双孢蘑菇菌丝蛋白的研究

柠檬酸工业废液中含有一定有效物质,如糖类、蛋白质、矿物质等,可供微生物利用。本文介绍了利用废液深层培养双孢蘑菇菌丝蛋白的可能性及条件。并用1吨发酵堆进行生产试验,探讨了生产工艺条件,分析了发酵液中底物、产物的动态变化。双孢蘑菇菌丝蛋白培养温度25℃,底物 pH6,发酵周期6天,菌球直径1～3mm,菌球数量4.8×10~3个/ml,菌丝温重达底物量的29.3%。在上述条件下,发酵液中菌球直经、菌丝数量、发酵液粘度受生物剪切作用的影响,粘度大,剪切作用大,菌球直径小,菌球数量则多。本文为新型单细胞蛋白的开发,促进物质的多层次利用,消除环境污染,提供了一个新途径。     

浅谈提高高寒山区大豆单产的途径

本文分析了限制黑河地区大豆单产提高的原因，其原因是盲目引种、密度不适宜、肥效降低、病虫危害严重及重迎茬问题。提出了提高高寒山区大豆单产的方法。     

我国物流行业上市公司资本结构影响因素实证研究

采用实证分析和规范分析相结合的方法来研究影响物流行业上市公司资本结构的内外因素，并提出相应的对策与建议，以期能为物流行业上市公司制定合理的资本结构提供实证数据和参考。     

犬瘟热及细小满毒病例消化道呼吸道细菌的分离、培养鉴定及药敏试验

犬瘟热是由犬瘟热病毒引起的犬和许多肉食目动物的一种高度接触性传染病,以早期表现双相热,急性鼻卡他以及随后的支气管炎、卡他性肺炎,严重的胃肠炎和神经症状为特征.     

饲用塑性大豆浓缩磷脂流态化研究

研究了温度和水分含量对饲用大豆浓缩磷脂黏度的影响,发现Na 能提高浓缩磷脂的黏度,而Ca2 能降低浓缩磷脂的黏度。对于水分含量较低的塑性浓缩磷脂,添加0.4%的氯化钙或添加3%大豆油脱臭馏出物能显著降低低水分塑性浓缩磷脂的黏度,使其流态化。但对于高水分塑性浓缩磷脂,其作用不明显。0.3%的氯化钙和3%的大豆油脱臭馏出物复合使用能使塑性浓缩磷脂的黏度降到2000cPa.s以下,而且长时间保持稳定。     

重迎茬亚麻生长发育障碍机制的研究

重迎茬亚麻减产是由多种因素综合作用的结果,亚麻重迎茬可使病害加重是最主要因素,重茬年限越久发病越严重;重迎茬造成了土壤营养元素的单一消耗,随着重迎茬年限的增加,土壤中速效氮、速效磷、速效钾等含量降低。     

亚麻细菌病发生及防治技术研究

从亚麻细菌病的发生、发展、危害及病原茵特性到病害综合防治，进行了系统研究，明确了该病的发生与品种抗性、耕作栽培等诸方面的关系，为病害防治提供了理论依据；明确了种子带茵是该病害主要侵染源和传播途径，同时建立综合防病体系。