全文获取类型
收费全文 | 205篇 |
免费 | 8篇 |
专业分类
林业 | 24篇 |
农学 | 16篇 |
基础科学 | 2篇 |
62篇 | |
综合类 | 3篇 |
农作物 | 11篇 |
水产渔业 | 6篇 |
畜牧兽医 | 54篇 |
园艺 | 5篇 |
植物保护 | 30篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 8篇 |
2020年 | 2篇 |
2019年 | 6篇 |
2018年 | 8篇 |
2017年 | 5篇 |
2016年 | 8篇 |
2015年 | 4篇 |
2014年 | 7篇 |
2013年 | 22篇 |
2012年 | 11篇 |
2011年 | 10篇 |
2010年 | 7篇 |
2009年 | 8篇 |
2008年 | 7篇 |
2007年 | 10篇 |
2006年 | 7篇 |
2005年 | 7篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 10篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1979年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1973年 | 3篇 |
1969年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有213条查询结果,搜索用时 140 毫秒
1.
Maryam Moradi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269
Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity. 相似文献
2.
Bijan Soleymani Kamran Mansouri Mohsen Rastegari-Pouyani Shahram Parvaneh Fatemeh Khademi Mehdi Sharifi Tabar Ali Mostafaie 《Reproduction in domestic animals》2021,56(2):270-277
Separation of X and Y chromosome-bearing sperm is an appropriate method for the selection of desired sex of offspring to increase the profit in livestock industries. The purpose of this study was the production of a monoclonal antibody against recombinant bovine sex-determining region Y protein for separation Y sperm. The hybridoma cells from splenocytes of immunized female's balb/C mice and Sp2/0 cells were made. The binding affinity of our monoclonal antibody (mAbSRY2) was compared with mouse monoclonal SRY-15. The Western blot method indicated that mAbSRY2 successfully detected the rbSRY protein. The specificity and sensitivity of mAbSRY2 is comparable to SRY-15 commercially ones. The SRY gene in 100% of bull semen contains the Y chromosome that had the strongest binding affinity to mAbSRY2 was synthesized. In other words, the binding affinity of semen contains the X sperms near the negative control. In general, this immunological method can help to separate X from Y sperms. However, the mAbSRY2 is bind to Y-bearing sexed sperm, but in the future; the sexed sperms need to apply in farms. 相似文献
3.
S. Tripathi A. Chakraborty B. K. Bandyopadhyay K. Chakrabarti 《Archives of Agronomy and Soil Science》2013,59(4):439-450
The studied soils were collected from a 24-year long-term fertilizer experiment with rice (Oryza sativa L.) grown on tropical Typic Endoaquepts during monsoon season only. The organic carbon (OC) and total nitrogen (TN) status of the soils improved with the N100P22K42 treatment than the initial values at the start of the experiment. The available P status of the soils depleted in the N100P11K22 and N0P0K0 treatments than the initial value. Soil microbial biomass carbon (MBC) and basal soil respiration (BSR), activities of urease, acid and alkaline phosphatases as well as fluorescein diacetate hydrolyzing activity (FDHA) were the highest in the N100P22K42 treatment. The highest percentage of MBC in OC in the N100P22K42 treatment revealed the accumulation of organic matter with an overall improvement in nutrient status of soil, since MBC is a labile pool of plant nutrients. Long-term application of fertilizers (N100P22K42) had no deleterious effect on microbial and biochemical soil quality parameters. Rice grain yields were inconsistent with N100P0K0 and N0P0K0 while that of N100P22K42, N100P11K22 and N100P22K0 increased gradually during the course of the experiment for each block period of six years. The grain yield of rice showed high significant correlation with the microbial and biochemical soil parameters. 相似文献
4.
The objective of this study was to quantify inherent spatial variability and spatial cross-correlation of the van Genuchten retention parameters and saturated hydraulic conductivity (Ks) of surface and subsurface layers in a calcareous Inceptisols (Khuzestan province, Iran) under sugarcane cropping. Measurements were performed on 100-cm3 undisturbed soil cores collected at 94 locations along a 30-m-long transect with horizontal sampling distance intervals of 0.3 and 1 m at soil depths of 0–40 and 40–80 cm, respectively. Spatial variability was investigated using conventional statistics and geostatistical techniques. Coefficient of variation (CV) varied from 8.2% (for shape parameter, n at 40–80 cm depth) to 256.7% (for Ks at 0–40 cm depth). The n parameter and saturated water content, θs, showed a small-scale spatial heterogeneity with a maximum CV of 11.3% for the first depth and 9.2% for the second depth. Most of the hydraulic parameters at both depths showed a spatial structure and convex experimental semivariograms with dominant spherical models with the influence range of 3.2–41 m. In most cases, the extent of spatial correlation scales of cross-semivariograms for pairs of cross-correlated hydraulic variables was found to be different with reference to those relating to the direct semivariograms of correlated variables. 相似文献
5.
Rahul Tripathi A. K. Nayak R. Raja Mohammad Shahid S. Mohanty B. Lal 《Communications in Soil Science and Plant Analysis》2017,48(10):1154-1166
Proper doses of nitrogenous fertilizer are most important for rice production system because a large part of the nitrogen may be lost if it is not applied judiciously. A study was conducted covering five blocks of Balasore and two blocks of Bhadrak districts. Soil samples were collected randomly, and field visit was conducted during peak vegetative stage of rice. Two approaches have been used in this study for estimating the site-specific nitrogen (N) requirement in the study area. In one approach, geostatisical analysis and kriging was used to develop the soil test–based N recommendation map by which a minimum of 72 kg N ha?1 and maximum of 94 kg N ha?1 were recommended. In a second approach, remote sensing was used and N recommendation map was developed using the moderate-resolution imaging spectroradiometer (MODIS) leaf area index (LAI) and normalized difference vegetation index (NDVI) satellite data, and a minimum requirement of 60 kg N ha?1 and maximum of 120 kg N ha?1 was estimated through this approach. 相似文献
6.
Aditya Pratap Umashanker Prajapati Chandra Mohan Singh Sanjeev Gupta Meenal Rathore Nupur Malviya Rakhi Tomar Ajeet Kumar Gupta Swapnila Tripathi Narendra Pratap Singh 《Plant Breeding》2018,137(3):235-249
Grain legumes, the important constituents of sustainability‐based cropping systems and energy‐limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so‐called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest. The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement. 相似文献
7.
Rao GS Ramesh S Ahmad AH Tripathi HC Sharma LD Malik JK 《Veterinary research communications》2001,25(3):197-204
The pharmacokinetics of enrofloxacin and its active metabolite ciprofloxacin were investigated in goats after a single intramuscular administration of enrofloxacin at 2.5 mg/kg body weight. The plasma concentrations of enrofloxacin and ciprofloxacin were determined simultaneously by a HPLC method. The peak concentrations (C
max) of enrofloxacin (1.13 g/ml) and ciprofloxacin (0.24 g/ml) were observed at 0.8 and 1.2 h, respectively. The elimination half-life (t
1/2), volume of distribution (V
d(area)), total body clearance (ClB) and mean residence time (MRT) of enrofloxacin were 0.74 h, 1.42 L/kg, 1329 ml/h per kg and 1.54 h, respectively. The t
1/2, area under the plasma concentration–time curve (AUC) and the MRT of ciprofloxacin were 1.38 h, 0.74 g h/ml and 2.73 h, respectively. The metabolic conversion of enrofloxacin to ciprofloxacin was appreciable (36%) and the sum of the plasma concentrations of enrofloxacin and ciprofloxacin was maintained at or above 0.1 g/ml for up to 4 h. Enrofloxacin appears to be useful for the treatment of goat diseases associated with pathogens sensitive to this drug. 相似文献
8.
Bijan Payandeh 《New Forests》1991,5(1):1-12
Data from 122 northeastern Ontario plots were used to develop composite site-productivity functions for black spruce (Picea mariana [Mill.] B.S.P.). A logistic model produced the best fit to the data. The resulting equations produce sets of polymorphic site-productivity curves with subsets of three curves, one for each of Ontario's Forest Ecosystem Classification Operational Groups (FEC OGs) 11, 12 and 14. For direct estimates of site productivity, site index was expressed as a function of stand height, age and FEC OG by employing the reciprocal of the logistic model. Application of the resulting equations and tables is demonstrated in an example. The procedures employed may be used to develop ecologically based site-productivity functions for other areas. 相似文献
9.
Bijan Payandeh 《New Forests》1988,2(3):145-160
Drainage and fertilization of an existing peatland black spruce (Picea mariana (Mill.) B.S.P.) stand and 24 possible methods of planting a clear-cut area near Cochrane, Ontario were evaluated by means of a recently developed forestry investment decision model, FIDME.Results indicate that, on the basis of the cost estimates used and assumptions made, drainage of the existing stand is the most economical treatment if it can reduce the rotation age by 30 years or more. Drainage combined with fertilization is the second best alternative if this treatment can reduce the rotation age by 40 years or more. Fertilization of the undrained site ranks a distant third, and then only if it reduces the rotation age by 10 years; otherwise, it will be uneconomical to apply.In the case of the clear-cut area, planting bare-root stock on a mechanically prepared but undrained site without weed control ranked first among the 24 alternatives considered; the same treatment, but with weed control included, ranked second. Planting containerized seedlings on a mechanically prepared but undrained site with weed control ranked third, followed by planting bare-root stock on a mechanically prepared and drained site with weed control. In contrast, planting containerized seedlings on an unprepared site and with no weed control ranked as the least cost-effective method considered. 相似文献
10.
Factors affecting urine reagent strip blood results in dogs and nonhuman primates and interpretation of urinalysis in preclinical toxicology studies: a Multi‐Institution Contract Research Organization and BioPharmaceutical Company Perspective
下载免费PDF全文
![点击此处可从《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)