Secondary Metabolites from Marine-Derived Fungi and Actinobacteria as Potential Sources of Novel Colorectal Cancer Drugs

Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one of the most common methods used for the pharmacological treatment of this cancer patients. Nevertheless, the adverse effect of chemotherapy is not optimized for improving the quality of life of people who are older, who are the most vulnerable subpopulation. This review presents recent updates regarding secondary metabolites derived from marine fungi and actinobacteria as novel alternatives for cytotoxic agents against colorectal cancer cell lines HCT116, HT29, HCT15, RKO, Caco-2, and SW480. The observed marine-derived fungi were from the species Aspergillus sp., Penicillium sp., Neosartorya sp., Dichotomomyces sp., Paradendryphiella sp., and Westerdykella sp. Additionally, Streptomyces sp. and Nocardiopsis sp. are actinobacteria discussed in this study. Seventy one compounds reviewed in this study were grouped on the basis of their chemical structures. Indole alkaloids and diketopiperazines made up most compounds with higher potencies when compared with other groups. The potency of indole alkaloids and diketopiperazines was most probably due to halogen-based functional groups and sulfide groups, respectively.     

Influence of intermediate deformation rates in softwoods characterized with fracture resistance R-curves

Fracture resistance curves of Norway spruce were evaluated over a range of crosshead speeds between 0.05 and 200 mm/min using compact tension specimens. The fracture resistance curves were determined via J-integral K _R versus Δa (crack extension) using hybrid experimental–finite element method with stepwise quasi-static and transient dynamic analysis. Coupling experiments and high-speed camera with a rate of 0.5–135 frames/s capture enabled determination of crack kinetics and velocities. Digital image correlation method was employed to evaluate strain distribution maps of the fracture process zone. Deformation measurements suggested strong strain localization and significant influence of the loading rate. The most distinct strain map was evaluated for the loading rate of 200 mm/min, suggesting inertial effects. Rising fracture resistance develops for smaller crack lengths (0.15–0.20 a/w) for all loading rates, with an increase of up to 20–50% compared to initiation values. Fracture resistance R-curves were evaluated for single individual specimens as well as for averaged load–deformation responses, which hide strong localisations in single responses. Influence of deformation rate on the shape and magnitude of fracture resistance curves is significant. Inertial effects in transient dynamic response give rise to twofold higher fracture resistance of softwoods compared to quasi-static resistance.