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Houessou JK Benac C Delteil C Camel V 《Journal of agricultural and food chemistry》2005,53(4):871-879
The presence of polycyclic aromatic hydrocarbons (PAHs) in coffee has been reported and is suspected to be due to the degradation of coffee compounds during the roasting step. Due to the high toxicity of these compounds, among which benzo[a]pyrene is known to be the most carcinogenic, their presence in the coffee, especially the coffee brew that is directly ingested by the consumer, is of prime importance. However, due to the low solubility of these compounds, their concentrations are expected to be rather low. As a consequence, reliable and sensitive analytical methods are required. The aim of this study was to develop a reliable and fast analytical procedure to determine these organic micropollutants in coffee brew samples. PAHs were retained on a 0.5 g polystyrene-divinylbenzene cartridge before being eluted by a mixture of methanol/tetrahydrofuran (10:90 v/v), concentrated, and directly analyzed by reversed-phase high-performance liquid chromatography coupled to a fluorescence detector. Application to the determination of PAHs in several coffee brew samples is also given, with mean estimated concentrations in the range of 0-100 ng L(-1) for suspected benzo[b]fluoranthene and benzo[a]pyrene, whereas no fluoranthene could be detected. Tentative identification was made on the basis of UV spectra. However, identification of the suspected traces of PAHs could not be achieved due to matrix effects, so that the presence of coeluting compounds may not be excluded. 相似文献
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Sample treatment procedures were tested for the determination of polycyclic aromatic hydrocarbons (PAHs) in ground coffee. Pressurized liquid extraction (PLE), under different conditions, was combined with several cleanup methods, namely in situ purification, C18-silica solid-phase extraction (SPE), silica SPE, acid digestion, and alkaline saponification. Soxhlet extraction and direct alkaline saponification were also tested. Best results were obtained using PLE with hexane/acetone 50:50 (v/v) under 150 degrees C. Alkaline saponification followed by cyclohexane extraction and silica SPE was required to eliminate interferent compounds. Finally, 11 PAHs could be quantified in ground coffee with limits of detection in the range of 0.11-0.18 microg kg(-1). Application to ground Arabica coffee lots from Colombia revealed the presence of several PAHs, giving an overall toxicity equivalence in the range of 0.16-0.87 microg kg(-1). PAH identification was performed using both high-performance liquid chromatography-diode array detection and gas chromatography coupled to mass spectrometry. 相似文献
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Amandine Gasc Benjamin L. Gottesman Dante Francomano Jinha Jung Mark Durham Jason Mateljak Bryan C. Pijanowski 《Landscape Ecology》2018,33(8):1399-1415
Context
While remote sensing imagery is effective for quantifying land cover changes across large areas, its utility for directly assessing the response of animals to disturbance is limited. Soundscapes approaches—the recording and analysis of sounds in a landscape—could address this shortcoming.Objectives
In 2011, a massive wildfire named “the Horseshoe 2 Burn” occurred in the Chiricahua National Monument, Arizona, USA. We evaluated the impact of this wildfire on acoustic activity of animal communities.Methods
In 2013, soundscape recordings were collected over 9 months in 12 burned and 12 non-burned sites in four ecological systems. The seasonal and diel biological acoustic activity were described using the “Bioacoustic Index”, a detailed aural analysis of sound sources, and a new tool called “Sonic Timelapse Builder” (STLB).Results
Seasonal biophony phenology showed a diurnal peak in June and a nocturnal peak in October in all ecological systems. On June mornings, acoustic activity was lower at burned than at non-burned sites in three of four ecological systems, due to a decreased abundance of cicadas directly impacted by the death of trees. Aural analyses revealed that 55% of recordings from non-burned sites contained insect sounds compared to 18% from burned sites. On October nights, orthopteran activity was more prevalent at some burned sites, possibly due to post-fire emergence of herbaceous.Conclusions
Soundscape approaches can help address long-term conservation issues involving the responses of animal communities to wildfire. Acoustic methods can serve as a valuable complement to remote sensing for disturbance-based landscape management.5.
Dominique Hazard Tiphaine Macé Amandine Kempeneers Eric Delval Didier Foulquié Jacques Bouix Alain Boissy 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(4):374-383
In livestock, improving maternal reactivity towards the litter is an important issue in breeding strategies to promote production and animal welfare. As of yet, no studies have investigated the within-breed genetic variation of maternal reactivity in sheep. The objective of this study was to estimate the genetic parameters of maternal reactivity traits. A total of 1,095 primiparous and 1,441 multiparous Romane ewes were phenotyped 24 hr postlambing using a behavioural test (arena test, AT) over a 10-year experimental period. The test consisted of three successive phases evaluating the ewe's attraction to her litter, reactivity to separation from her litter, and reactivity to a conflict between attraction to her litter and avoidance of a motionless human. The ewes were reared exclusively on rangelands (South of France) and lambed outdoors in the spring. High-pitched bleating and low-pitched bleating in the AT were mostly highly heritable (0.39‒0.46). Heritabilities were moderate for proximity to the litter in the presence of a human (0.27) and low for locomotion and vigilance in the AT (0.09‒0.15). The measurements of a given behaviour in the three phases of the AT were highly genetically correlated. Few genetic correlations were found between the different behavioural traits in the AT, the highest correlations being between high-pitched bleating and low-pitched bleating (−0.43 to −0.77). In conclusion, our findings demonstrate moderate-to-high heritability for maternal reactivity traits. These traits could be included in genetic selection schemes to enhance maternal attachment provided there is no unfavourable link with other production traits. 相似文献
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Romisa Asadi Rachid Elaini Renaud Lacroix Thomas Ant Amandine Collado Lucy Finnegan 《国际虫害防治杂志》2020,66(2):182-193
AbstractThe Mediterranean fruit fly, Ceratitis capitata, (Medfly) is considered a key pest of citrus fruit in many countries. Integrated pest management (IPM) approaches are frequently highly dependent on the use of insecticides. Oxitec’s self-limiting (prevents the insects’ offspring from surviving to adulthood) Medfly technology (OX3864A) offers an approach to manage Medfly outbreaks and may offer improved sustainability and economics for area-wide programmes. Netted cages were used to evaluate OX3864A deployed for the first time outdoors as a preventative treatment to suppress wild Medfly. Comparative assessments examined sexual competitiveness, the relative performance of OX3864A releases at both adult and pupal life-stages, and the ability of OX3864A to protect against fruit damage. OX3864A males are as competitive as wild males. Deployment of both adult and pupal life-stages of OX3864A effectively reduce Medfly abundance, resulting in elimination of the target pest populations. OX3864A deployment as adults also demonstrated the beneficial attribute of protecting fruit quality to preserve marketable yield. Data for adult and pupal deployment strategies are encouraging for self-limiting technologies targeting Medfly populations and support a broader evaluation of OX3864A Medfly or further improved self-liming Oxitec strains of Medfly in open settings. 相似文献
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