Prevalence of avian influenza (H9N2) in commercial quail,partridge, and turkey farms in Iran, 2014–2015

Tropical Animal Health and Production - Avian influenza virus (AIV) H9N2 subtype is endemic in Iran and causes substantial economic loss to the growing poultry industry within the country. In this...     

Effects of in ovo injection of chrysin,quercetin and ascorbic acid on hatchability,somatic attributes,hepatic oxidative status and early post‐hatch performance of broiler chicks

An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post‐hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18‐day‐old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0.05 ml distilled water; (iii) 0.05 ml distilled water containing 6 mg ascorbic acid; (iv) 0.05 ml dimethyl sulfoxide (DMSO); (v) 0.05 ml DMSO containing 4.5 mg quercetin; and (vi) 0.05 ml DMSO containing 4.5 mg chrysin. The hatchability rate, hatching weight, residual yolk sac weight, yolk sac‐free body weight, liver weight, hepatic glutathione peroxidase and total superoxide dismutase activities, as well as malondialdehyde concentrations, were not affected by the injected solutions. There were no differences between chicks hatched from the control and in ovo injected eggs in weight gain, feed intake and feed conversion ratio from 0 to 11 days of age. However, the specific contrast performed between the in ovo injected groups and intact eggs revealed that in ovo injection significantly increased hatchability rate (p = .0493). This finding also implies that our injection procedure was harmless. In conclusion, the intra‐egg injection of chrysin, quercetin or ascorbic acid at the injection rates used in this study did not have a significant effect on hatchability, somatic characteristics, early growth performance and hepatic antioxidant status of broiler chicks. However, the overall hatchability was higher in the in ovo injected eggs as compared to non‐injected ones. These findings also confirmed the harmlessness of the procedure developed for in ovo injection in this study.     

The suitability of some blood gas and biochemical parameters as diagnostic tools or early indicators of ascites syndrome in broiler sire lines

In recent few years, there have been some attempts to find a reliable indicator trait as a selection criterion against susceptibility to ascites syndrome (AS). Blood parameters were of great interest as they could be measured in live animals without implementing an ascites‐inducing challenge (AIC). In this work, the suitability of some blood parameters was evaluated for diagnosing AS‐susceptible chicks in later steps of the disease in trial 1 as well as their early predictive ability in trial 2. In the first trial, one hundred 1‐day‐old chicks from two pure broiler lines namely S₁ and S₂ and, in the second trial, 226 1‐day‐old chicks from line S₂ were subjected to AIC. Saline drinking water (1200 mg/l) and lower‐than‐standard ambient temperatures were the implemented AICs in trials 1 and 2 respectively. The blood parameters including pH, partial pressure of O₂ (pO₂), partial pressure of CO₂ (pCO₂), bicarbonate ion concentration (BIC), percentage of haematocrit (HCT) and saturated haemoglobin (SaO₂) were measured twice per each bird at days 28 and 35 in trial 1 and once in trial 2 at day 21. The results of the first trial revealed that in line S₂ some of the blood parameters differed significantly between the ascitic and non‐ascitic groups following exposure to AIC. In this line, the incidence of AS was accompanied by a lower pO₂, SaO₂ and BIC, while with higher pCO₂ and HCT values. In the second trial, however, although almost all of the parameters showed meaningful differences between the ascitic and non‐ascitic broilers, only mean difference of BIC parameter was statistically significant. The general conclusion of this study is that the blood parameters can somewhat have diagnostic ability in the condition in which the AIC is already present, whereas the results did not approve their usefulness as early predictors of AS.     

Eosinophilic leukaemia in a cat

A 14-year-old female domestic shorthair cat was presented to Tehran University Veterinary Teaching Hospital for a persistent fever, anorexia, intermittent vomiting, weight loss and weakness. The main clinical signs were pale mucous membranes, dehydration and splenomegaly. The complete blood count and serum biochemistry tests revealed non-regenerative anaemia, thrombocytopenia and increased alkaline phosphatase (ALP) activity. An enzyme-linked immunosorbent assay (ELISA) test for feline leukaemia virus was negative. Blood film and bone marrow examination revealed a large number of immature eosinophils with variable sizes and numbers of faintly azurophilic granules. Cytochemical staining of blood film demonstrated 70% positive cells for ALP activity. Four percent CD34 positive cells were detected by flow cytometry. As eosinophilic leukaemia is difficult to identify by light microscopy, well-defined diagnostic criteria and the use of flow cytometry and cytochemical staining can improve the ability to correctly diagnose this type of leukaemia in cats.     

Improvement of Seed Quality of Wheat (Triticum aestivum) as Affected by Brassinolide under Different Irrigation Regimes

Journal of Crop Science and Biotechnology - Irrigation interruption owing to time of incidence is affecting the seed quality. The aim of this research was to determine whether the brassinolide and...     

Response of wheat genotypes to foliar spray of ZnO and Fe2O3 nanoparticles under salt stress

This study evaluated the effects of iron oxide (Fe₂O₃) and zinc oxide (ZnO) on two wheat genotypes (Kavir and Tajan) at three levels (0, 75, and 150 mM sodium chloride (NaCl)) of salinity. Spray treatments included two forms of normal and nanoparticles of Fe₂O₃ and ZnO, a mixture of nanoparticles of Fe₂O₃ and ZnO (2 g L^?1) and a non-spray treatment. The pot experiment was arranged as factorial in a randomized complete block design with four replications. Two forms of Fe₂O₃ and ZnO significantly accelerated plant height, leaf area, shoot dry weight, and the concentration of iron (Fe) and zinc (Zn) in comparison with non-spray treatment. The highest plant height and leaf Fe concentration belonged to Fe₂O₃ nanoparticles; however, it seems that the spray of nanoparticles may not be superior compared with normal forms in alleviation of salinity impacts.     

Analysis of the variations in dry matter yield and resource use efficiency of maize under different rates of nitrogen,phosphorous and water supply

Abstract

Increasing resources use efficiency in intensive cultivation systems of maize (Zea mays L.) can play an important role in increasing the production and sustainability of agricultural systems. The objectives of the present study were to evaluate DM yield and the efficiency of inputs uses under different levels of water, nitrogen (N) and phosphorus (P) in maize. Therefore, three levels of irrigation including 80 (ETc₈₀), 100 (ETc₁₀₀) and 120% (ETc₁₂₀) of crop evapotranspiration were considered as the main plots, and the factorial combination of three levels of zero (N₀), 200 (N₂₀₀) and 400 (N₄₀₀) kg N ha^?1 with three levels of zero (P₀), 100(P₁₀₀) and 200 (P₂₀₀) kg P ha^?1 was considered as the sub plots. The results showed that increasing the consumption of water and P was led to the reduction of N and P utilization efficiency, while RUE increased. WUE was also increased in response to application of N and P, but decreased when ET_C increased. DM yield under ETc₈₀ treatment reduced by 11 and 12%, respectively, compared to ETc₁₀₀ and ETc₁₂₀ which was due to reduction of cumulative absorbed radiation (R_abs(cum)) and RUE. Under these conditions, changes of stomatal conductance (gs) had little effect on DM yield. It was also found that N limitation caused 11 and 20% reduction in DM yield compared to N₂₀₀ and N₄₀₀, respectively. This yield reduction was mainly the result of decrease in RUE. By decreasing R_abs(cum), P deficiency also reduced DM yield by 5 and 9%, respectively, relative to P₁₀₀ and P₂₀₀ treatments.     

Age estimation of Arabian mares by incisors morphometry and dentition changes

Veterinary Research Communications - Accurate estimation of a horse's age based on the condition of the tooth status is necessary as a scientific and artistic technique, which has not been...     

Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens

This experiment was conducted to evaluate the effects of different levels of sweet orange (Citrus sinensis) peel extract (SOPE) on humoral immune system responses in broiler chickens. Three hundred 1‐day broilers (Ross‐308) were randomly allocated to treatments varying in supplemental SOPE added in the drinking water. The experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of SOPE and a treatment containing 1250 ppm of SOPE. All treatments were isocaloric and isonitrogenous. Broilers were vaccinated with Newcastle disease virus (NDV), avian influenza (AI), infectious bursal disease (IBD) and infectious bronchitis virus (IBV) vaccines. Antibody titer response to sheep red blood cells (SRBC) was higher in the group fed 1250 ppm of SOPE (P < 0.05) as well as for immunoglobulin G (IgG) and IgM. Similarly, antibody titer responses to all vaccines were constantly elevated (P < 0.05) by SOPE enrichment in a dose‐dependent manner. Relative weights of spleen and bursa of Fabricius were unaffected by treatments. Dietary SOPE supplementation may improve the immune response and diseases resistance, indicating that it can constitute a useful additive in broiler feeding. Thus, supplying SOPE in rations may help to improve relative immune response in broiler chickens.