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Hu J Shibata Y Voss C Shemesh T Li Z Coughlin M Kozlov MM Rapoport TA Prinz WA 《Science (New York, N.Y.)》2008,319(5867):1247-1250
The tubular structure of the endoplasmic reticulum (ER) appears to be generated by integral membrane proteins, the reticulons and a protein family consisting of DP1 in mammals and Yop1p in yeast. Here, individual members of these families were found to be sufficient to generate membrane tubules. When we purified yeast Yop1p and incorporated it into proteoliposomes, narrow tubules (approximately 15 to 17 nanometers in diameter) were generated. Tubule formation occurred with different lipids; required essentially only the central portion of the protein, including its two long hydrophobic segments; and was prevented by mutations that affected tubule formation in vivo. Tubules were also formed by reconstituted purified yeast Rtn1p. Tubules made in vitro were narrower than normal ER tubules, due to a higher concentration of tubule-inducing proteins. The shape and oligomerization of the "morphogenic" proteins could explain the formation of the tubular ER. 相似文献
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Xiaoge Zhou Zhaoyi Mo Yankun Li Liang Huang Sihai Yu Lan Ge Yamei Hu Shengjie Shi Lutong Zhang Liguang Wang Lei Gao Gongshe Yang Guiyan Chu 《畜牧与生物技术杂志(英文版)》2022,(3):756-768
Background: Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state, and it may have detrimental effects on female reproduction and fertility. Oleic acid induces lipid accumulation in bovine granulosa cells, which leads to a foam cell-like morphology and reduced steroidogenesis.However, why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear. This study focused on oleic acid’s effects on lipid type and steroidog... 相似文献
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Lin CT Hu CK Liu CH Yeh LS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):847-850
Surgical outcome and complications following evisceration and implantation of intraocular silicone prosthesis (ISP) in 20 canine eyes with end-stage glaucoma were analyzed, including clinical signs, complications, cosmetic appearance, and owners' responses. The mean postoperative follow-up time was 312 days. Major short-term surgical complications were central corneal ulceration (6/20) and infections (3/20), while long-term complication was keratoconjunctivitis sicca (KCS) (2/20). All patient owners (100%) showed satisfied with the surgical outcome and postoperative cosmetic effect including 85% of the owners gave an excellent or good rating of satisfaction. No more medication needed for long term control except the KCS cases. With careful case selection and post-operative care, evisceration with ISP implantation proves a good and safe surgical procedure with minimal complications for end stage glaucoma. 相似文献
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Magnetorheological materials are a class of smart materials whose reological properties may be rapidly varied by application of magnetic field. By a simple mechanical mold, a visco-plastic model for the materials is developed. It is shown that the model can describe the variation of the mechanical properties for the Magnetorheological materials in the different magnetic field. The result is shown that the model can characterize the mechanical properties of Magnetorheological Materials in plastic and viscous variation. 相似文献
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To construct data warehouse with heterogeneous, self-governing and distributed database system is a challenge. Two problems have to be solved: one is how to collect data from heterogeneous data sources by an effective method, the other is how to clear up and change the format of the data collected from data sources. The opening and the interact-operation property of Web Services is Analyzed. A Data Collection System based on Web Services, which communicates with heterogeneous data sources with SOAP and HTTP, has been designed. A Data Collector is introduced to clear up and to transform data with composition technology. The pivotal XML technology of transforming and loading up the data are discussed. 相似文献