大豆DNA导入玉米后代植株蛋白质和油分的分析

[目的]探讨不同科之间DNA导入后的性状变异情况,并从中筛选优良稳定变异株系。[方法]利用花粉管通道法将大豆总DNA导入普通玉米自交系7313,逐代选择,至玉米田间性状、籽粒颜色、形状、果穗轴色等均稳定,对筛选出的变异株系果穗籽粒进行粗蛋白、醇溶蛋白、谷蛋白和油分含量的测定和比较。[结果]株系26h-4-3的籽粒粗蛋白、醇溶蛋白、谷蛋白和油分含量与对照相比差异都达到显著或极显著水平,在D3和D4代的提高幅度分别为10.34%和26.70%、6.58%和6.28%、15.09%和70.34%5、5.82%和51.52%;株系26h-3-1的各项指标与对照相比也有极大提高,在D3和D4代的提高幅度分别为5.67%和21.63%、1.91%和2.31%、10.85%和62.27%2、2.49%和9.67%。[结论]26h-4-3和26h-3-1变异株系的粗蛋白、醇溶蛋白、谷蛋白和油分含量都稳定提高,是符合高蛋白育种目标的优良变异株系。     

大豆DNA导入玉米后代植株蛋白质和油分的分析(英文)

[Objective] The experiment was aimed to explore character variation between different families after DNA introduction and select variant plants with good stability. [Method] The method of pollen-tube-pathway was used to introduce total DNA of soybean into normal maize inbred line 7313 for selecting generation by generation. When field characters of maize, grain colors, grain traits and panicle axis colors were stable, the crude protein, gliadin, glutelin and oil content of grains which were selected from variant strains were detected and compared. [Result] The grain crude protein, gliadin, glutelin and oil content of line 26h-4-3 were significantly different from these of control treatment. The increments of D3 and D4 generation were 10.34% and 26.70%, 6.58% and 6.28%, 15.09% and 70.34%, 55.82% and 51.52% respectively. All indexes of line 26h-3-1 were also higher than these of control treatment and the increments of D3 and D4 generation were 5.67% and 21.63%,1.91% and 2.31%, 10.85% and 62.27%,22.49% and 9.67%. [Conclusion] The crude protein, gliadin, glutelin and oil content of variant line 26h-4-3 and 26h-3-1 were stable, so variant line 26h-4-3 and 26h-3-1 were excellent variant strains which satisfied the requirement of high protein breeding.     

果蔗新品系粤东8号脱毒组培繁育技术

果蔗脱毒健康种苗可有效去除宿根矮化病、花叶病等种苗病害病原菌,在生长速度、株高、节问长度、蔗茎产量等方面均比常规种苗有显著提高.本研究以优良果蔗新品系粤东8号为试验材料,从外植体选择、减毒处理、无菌苗获得、病原体检测、继代增殖、生根炼苗、假植移栽等方面介绍了粤东8号果蔗脱毒组培苗的繁育技术,为其大量生产提供技术依据.     

袋式培养在剑麻组培苗生产中的应用

利用塑料薄膜袋代替玻璃瓶的袋式培养剑麻组培苗,降低了容器一次性投入的78.3 %,免去玻璃瓶的存储仓库和玻璃瓶的搬运、清洗等费用。培养基节约33.3 %,配制与消毒人工费节约40 %,消毒效率提高2.08倍,培养空间利用率提高46.8 %以上,接种效率提高12.5 %,组琣苗污染率降低7.1 %以上,生根苗的移栽成活率提高7.6 %以上,组培苗的出苗效率和运输效率提高2倍以上。