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Hyunkyoung LEE Hyeshin HWANG Younghye RO Ji-Hyeon KIM Kyunghyun LEE Eunjin CHOI Youchan BAE Byungjae SO Inhyung LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):869
This study was performed to examine and clarify the cause of hindlimb ataxia and neuropathy seen in the South Korean horse population. Fifty horses diagnosed with hindlimb ataxia and neuropathy were referred for this study. Neurological examination was performed on 47 horses while necropsy was performed in all 50 animals. The occurrence of neurological diseases increased rapidly in the summer and 47 out of 50 horses were referred after the end of July. The incidence of neurological diseases started from the southern part of Korea in July and proceeded northward in August and September. Although there was no correlation with age, Thoroughbred and Warmblood horses showed a higher incidence rate than Halla and Jeju horses. The incidence rate was 5 times higher in geldings than in mares and stallions. Of the 20 cases, 16 were diagnosed with eosinophilic meningoencephalomyelitis in 2015. The most common lesions observed in 2016 were parasitic meningoencephalomyelitis (10 cases, 33%) and eosinophilic meningomyelitis (7 cases, 23%). Histopathological analysis of the brain and spinal cord revealed nematodes of approximately 100–200 µm in diameter, microcavitation and infiltrates of eosinophils, and brown pigmented macrophage infiltrates. The nematodes were identified as Setaria digitata via DNA sequencing, performed subsequent to polymerase chain reaction using DNA isolated from formalin-fixed paraffin-embedded tissue sections of the spinal cord. These results show that aberrant migration of Setaria digitata larva in the brain and spinal cord was a major cause for neurological signs in horses. 相似文献
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The feeding value of fresh pasture grazed in situ is determined by animal performance or productivity and could be relatively easily established for growing and lactating horses. Despite this, there is a lack of published information on the relative feeding value of different pastures and forages grazed by horses in New Zealand and the world. In addition, for adult breeding or non-breeding and young or adult sport or performance horses, the definition of feeding value and its determination remain problematic. Limited information suggests that the feeding value of perennial ryegrass-based pasture in New Zealand for young growing horses is high, and growth rates for Thoroughbred horses fed solely on pasture in New Zealand are similar to those reported from the Northern Hemisphere where grain-based supplements are fed in addition to pasture or other forages. Attempts to assess the ability of fresh pastures to meet the nutrient requirements of horses are hampered by problems associated with determination of feed intake by grazing horses and lack of knowledge of the digestibility and utilisation of digested nutrients, including the relative bio availability of macro- and micro-minerals in pasture. A further challenge for future research is to determine the effect of herbage allowance and grazing behaviour, including pasture species preferences, on voluntary feed intake by grazing horses. Grazing pasture has benefits for equine health and well-being including reduced risk of some nutrition-related disorders and reduced prevalence of stereotypic behaviour. Pastured horses have greater freedom for expression of natural behaviours including social interaction and exercise. However, grazing pasture is also associated with animal health problems, particularly parasitism and diseases related to pasture-associated toxins. 相似文献
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The effect of bulk density, water content and soil type on the diffusion of chloride in soil 总被引:1,自引:0,他引:1
The relative importance of soil bulk density, water content and potential on the self-diffusion and impedance factors of 36 C1 in a sandy loam and loamy clay were studied. The soil bulk densities used represented a range of conditions from freshly tilled seedbeds to compacted soils. The volumetric water contents and pF were the main factors controlling the soil impedance factors, with bulk density making a small but significant contribution.
Soil type affects the impedance factors through differences in anion exclusion volumes, the water contents of poorly connected pores that contribute little to the diffusion process, and tortuosity of the diffusion pathways. 相似文献
Soil type affects the impedance factors through differences in anion exclusion volumes, the water contents of poorly connected pores that contribute little to the diffusion process, and tortuosity of the diffusion pathways. 相似文献
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Yutaka SUZUKI Sang‐Houn SONG Katsuyoshi SATO Kyoung‐Ha SO Astrid ARDIYANTI Shun KITAYAMA Yeon‐Hee HONG Sung‐Dae LEE Ki‐Choon CHOI Akihiko HAGINO Kazuo KATOH Sang‐gun ROH 《Animal Science Journal》2012,83(3):263-267
Accumulating data suggest a relationship between chemerin and energy metabolism. Our group previously described gene cloning, expression analysis and the regulatory mechanism of chemerin and its own receptor in mice and cattle. The objective of the present study was to investigate the physiological effect of chemerin on endocrine changes and energy metabolism in sheep using a biologically stable chemerin analog. The chemerin analog was intravenously administrated (100 or 500 µg/head) to sheep, and plasma insulin and metabolites (glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and high‐density lipoprotein (HDL) cholesterol) were analyzed. The chemerin analog dramatically increased the insulin levels, and glucose levels were decreased. NEFA levels were slightly decreased at 20 min but then increased gradually from 60 to 180 min after analog administration. In addition, injection of the chemerin analog immediately increased triglyceride and total cholesterol but not HDL levels. These results suggested that chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo. This study provides new information about endocrine and metabolic changes in response to chemerin in sheep. 相似文献
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CS Pinares-Patiño GC Waghorn RS Hegarty SO Hoskin 《New Zealand veterinary journal》2013,61(5):252-261
AbstractAIMS: The aims of this cross-sectional study were to investigate the herd and cow-level prevalence of bovine digital dermatitis (BDD) in dairy farms in the northern Taranaki region of New Zealand, and to identify whether there was any spatial clustering of herds with the disease.METHODS: A survey of 224 dairy farms in the northern Taranaki region of New Zealand was undertaken from September 2014 to February 2015. Following training in robust criteria to confirm BDD visually, a technician inspected the rear feet of every milking cow on the farms during milking. The identity of cows with lesions and the feet involved were recorded. The proportion of cows affected among the inspected population (cow-level prevalence), the proportion of a herd affected (farm-level prevalence), and proportion of farms with ≥1 cow with lesions, were calculated. A bivariate K function analysis was then used to assess whether farms with ≥1 cow with lesions were clustered, after accounting for the distribution of the farms involved in the study.RESULTS: Bovine digital dermatitis lesions were observed on 143/224 (63.8 (95% CI=57.5–70.1)%) farms. Within-farm prevalence was 0% on 81 (36.2%) farms, between >0 and <3% on 120 (53.5%) farms, with a maximum prevalence of 12.7% on one farm. Overall, cow-level prevalence was 707/60,455 (1.2 (95% CI=0.9–3.0)%), and on affected farms was 707/41,116 (1.7 (95% CI=1.4–2.1)%). In affected cows, 268/707 (37.9%) had a lesion on left foot only, 262/707 (37.1%) on the right foot only and 177/707 (25.0%) on both feet. The K function analysis showed no evidence of clustering of farms with BDD.CONCLUSIONS: Bovine digital dermatitis was widespread among the survey farms, but there was no evidence that there was any clustering of herds with BDD. The cow-level prevalence on affected farms was much lower than reported elsewhere.CLINICAL RELEVANCE: Although the prevalence at the cow level was low, if these data are representative of other regions of New Zealand, BDD could easily become a major problem on dairy farms in New Zealand, as has been observed in other countries. 相似文献
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RW Davis LA Fuiman TM Williams SO Collier WP Hagey SB Kanatous S Kohin M Horning 《Science (New York, N.Y.)》1999,283(5404):993-996
The hunting behavior of a marine mammal was studied beneath the Antarctic fast ice with an animal-borne video system and data recorder. Weddell seals stalked large Antarctic cod and the smaller subice fish Pagothenia borchgrevinki, often with the under-ice surface for backlighting, which implies that vision is important for hunting. They approached to within centimeters of cod without startling the fish. Seals flushed P. borchgrevinki by blowing air into subice crevices or pursued them into the platelet ice. These observations highlight the broad range of insights that are possible with simultaneous recordings of video, audio, three-dimensional dive paths, and locomotor effort. 相似文献
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Efficacy of montelukast in the treatment of chronic obstructive pulmonary disease in five horses 总被引:1,自引:0,他引:1
Five horses with moderate to severe chronic obstructive pulmonary disease (COPD) were treated with 0.11 (0.01) mg/kg bodyweight of montelukast, a cysteinyl leukotriene receptor antagonist, once a day for 26 days. The horses were evaluated clinically and endoscopically and subjected to arterial blood gas analysis and lung function tests before and after the period of treatment, and the plasma concentrations of montelukast were determined by high-performance liquid chromatography with fluorescence detection. The treatment did not result in statistically significant differences in the total scores of clinical and endoscopical signs, or in the difference in the arterioalveolar partial pressure of oxygen, maximal changes in pleural pressure, pulmonary resistance or dynamic compliance. The mean (sd) peak plasma concentration (C(max0) of montelukast was 12 (4) ng/ml and was reached 66 (13) minutes (t(max)) after its oral administration. The dose of montelukast per kg bodyweight was approximately the same as that for human beings, but the C(max) in the horses was 28 times lower and the t(max) was reached in one-fifth of the time, suggesting that its oral bioavailability may be lower. 相似文献
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为研究西藏绒山羊羊绒细度,以及了解相关候选基因对绒山羊产绒性能的影响,本研究对筛选chi-miR-105a靶基因进行验证,以西藏绒山羊为研究对象,联合RNA测序数据和蛋白质组学数据,筛选与羊绒细度相关的关键候选基因,并通过实时荧光定量和双荧光素酶报告基因试验对候选基因进行调控作用验证。结果表明:1)通过转录组和蛋白组数据整理得到384个DE mRNAs,12个DE miRNAs和29个DEPs。通过多组学联合分析发现CXCL10、SRC、CXCL9、FOS、ETV6、GMPS和PIP5K1B基因与羊毛细度相关。2)通过DEG-DE miRNA互作网络图发现chi-miR-105a与靶基因ETV6和PIP5K1B均在网络当中出现。因此对该调控因子进行验证和分析,根据靶基因表达水平试验发现转染chi-miR-105a mimics后引起毛乳头细胞中ETV6基因的mRNA表达显著降低(P<0.001),而PIP5K1B基因的表达量均无显著变化。3)通过双荧光素酶报告基因试验表明,过表达chi-miR-105a后,ETV6酶活性显著降低,即chi-miR-105a可与ETV6的3′UTR区结合。综上,通过多组学联合分析筛选出与羊毛细度相关基因CXCL10、SRC、CXCL9、FOS、ETV6、GMPS和PIP5K1B。通过双荧光素酶报告基因进行chi-miR-105a与其预测靶基因ETV6和PIP5K1B的靶标验证,确定chi-miR-105a是ETV6潜在的调控因子,本研究为加快优质绒山羊新品种(系)的培养提供理论依据。 相似文献