A comparison of the effects of carbon dioxide and medical air for abdominal insufflation on respiratory parameters in xylazine-sedated sheep undergoing laparoscopic artificial insemination

AIMS: To determine if abdominal insufflation with medical air will improve oxygenation and ventilation parameters when compared to insufflation with CO₂ in xylazine-sedated sheep undergoing laparoscopic artificial insemination (AI).

METHODS: Forty-seven sheep underwent oestrus synchronisation and were fasted for 24 hours prior to laparoscopic AI. Each animal was randomised to receive either CO₂ or medical air for abdominal insufflation. An auricular arterial catheter was placed and utilised for serial blood sampling. Respiratory rates (RR) and arterial blood samples were collected at baseline, after xylazine (0.1?mg/kg I/V) sedation, 2 minutes after Trendelenburg positioning, 5 minutes after abdominal insufflation, and 10 minutes after being returned to a standing position. Blood samples were collected in heparinised syringes, stored on ice, and analysed for arterial pH, partial pressure of arterial O₂ (PaO₂), and CO₂ (PaCO₂). The number of ewes conceiving to AI was also determined.

RESULTS: Repeated measures ANOVA demonstrated temporal effects on RR, PaO₂, PaCO₂ and arterial pH during the laparoscopic AI procedure (p<0.001), but no difference between insufflation groups (p>0.01). No sheep experienced hypercapnia (PaCO₂>50?mmHg) or acidaemia (pH<7.35). Hypoxaemia (PaO₂<70?mmHg) was diagnosed during the procedure in 14/22 (64%) ewes in the CO₂ group compared with 8/23 (35%) ewes in the medical air group (p=0.053). Overall, 15/20 (75%) ewes in the CO₂ group conceived to AI compared with 16/22 (72.7%) in the medical air group (p=0.867).

CONCLUSIONS AND CLINICAL RELEVANCE: There were no statistical or clinical differences in RR, PaO₂, PaCO₂, pH, or conception to AI when comparing the effects of CO₂ and medical air as abdominal insufflation gases. None of the sheep experienced hypercapnia or acidaemic, yet 42% (19/45) of sheep developed clinical hypoxaemia, with a higher percentage of ewes in the CO₂ group developing hypoxaemia than in the medical air group. Based on the overall analysis, medical air could be utilised as a comparable alternative for abdominal insufflation during laparoscopic AI procedures.     

The use of ultrasonography in the reproductive evaluation of boars

The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty‐six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles. The groups of animals that were evaluated showed no difference in the main semen parameters that were evaluated, except for the sperm volume, concentration of the ejaculated sperm and the supravital staining; the lowest figures were for the animals from the Piau breed (group 1). In relation to the testicular biometrics, Duroc animals (group 4) had a greater scrotal width compared to the other groups. But when we assessed the intensity of pixels of the testicles, there was a difference between groups. The groups 2 (finishing animals), 3 (Pietrain) and 4 had no difference between themselves. Group 3 had greater pixel intensity in relation to group 1. Of the 26 animals studied, five showed an abnormality during ultrasound evaluation, like hydrocele, hyperechoic mass in the testicular parenchyma, cyst in the head of the epididymis and the presence of fluid in the head and tail of the epididymis. The various animal groups studied did not differ in the principal reproductive parameters evaluated, showing that despite the great variability of reproductive traits between breeds and within the same breed, the breeding soundness evaluation, the more complete it is, is essential for the selection of breeders and the ultrasonography of the reproductive system becomes an important addition in this examination.     

Silage and milk production: a comparison between additives for silage of high digestibility

Three grass silages made in early June from S23 perennial ryegrass were compared in a 16-week feeding experiment with twelve Ayrshire cows. The silages were made from uniform herbage which received either formic acid (‘Add-F’) at the rate of 201 t^?1, or a formalin-sulphuric acid mixture (‘Syiade’) at rates of 2.0 and 4.4 1 t^?1. The silages were offered ad libitum either alone or supplemented with a cube containing 38% CP in the DM at the rate of 1.4 kg per 10 kg milk. The daily intakes of silage DM were not significantly different on the three silage treatments, and averaged 10.7, 11.0 and 11.0 kg per cow on the formic acid and the 2.0 and 44 1 t^?1 formalin-acid treatments respectively. The mean daily yields of milk were 15.1, 13.3 and 13.7 kg per cow in the unsupplemented treatments, and 18.2, 18.1 and 18.0 kg per cow in the supplemented treatments on the formic acid and the 2.0 and 44 1 t^?1 formalin-acid treatments respectively. On the basis of total animal production expressed in terms of metabolizable energy requirements, it was concluded that the differences between the three silages were small.     

Silage and milk production: a comparison between four rates of groundnut cake supplementation of silage of high digestibility

Grass silage made in late May from S24 perennial ryegrass was offered to twelve Ayrshire cows in a 16-week feeding experiment. The silage had a DM content of 25.3%, a pH of 3.91, and contained 16.8% CP and 68.4% digestible organic matter in the DM. The silage was fed ad libitum and was the sole feed in the control treatment. In the other three treatments the silage was supplemented with a cube offered at rates of 0.8, 1.4 and 2.0 kg per 10 kg milk. The cube contained 82.2% groundnut, plus molasses and minerals, and had 37.9% CP in the DM. The daily intake of silage DM was 11.4 kg per cow on average over the four treatments which did not differ significantly. The mean daily milk yields were 14.8 kg per cow in the control treatment, and 16.5, 18.2 and 18.4 kg in the 0.8, 1.4 and 2.0 kg supplement treatments respectively. The SNF, CP and lactose contents of the milk were Wghest on the 1.4 kg supplement treatment. It is concluded that with a high digestibility silage, there is little evidence to support the feeding of more than 1.4 kg of high-protein cubes per 10 kg milk in the declining phase of lactation.     

A comparison of continuous grazing systems for milk production

In two experiments conducted with Ayrshire dairy cows in 1974 and 1975 on swards of S23 perennial ryegrass, four systems of continuous grazing were investigated. The leys received 345 and 370 kg ha^-1 fertilizer nitrogen in 1974 and 1975 respectively and were stocked at the rate of five cows per ha. In 1974, a comparison was made between two systems which either integrated silage making with, or separated it from, the continuous grazing. The provision of a silage aftermath in late July increased milk yield after that time, but had no significant effect on milk yield over the 19-week experimental period. In 1975, a comparison between weekly and monthly applications of fertilizer N showed no significant differences between milk yields in a 20-week period. The feeding of supplementary concentrates from late July onwards significantly increased milk yield. The treatments in both years had only small and mainly non-significant effects on milk composition. On average, excluding the concentrate treatment, the annual output of milk was 10,800 kg ha^-1 using spring-calved cows.     

A computer-based system for the management of the rust disease of French prunes1

Rust (Tranzscheliu discolor) is the most important disease of French prunes in New South Wales (NSW), Australia. Infection by this fungus occurs only when leaves are wet. The length of time for which the leaves are wet and the temperature during such periods are major determinants of whether infection occurs and its likely severity. The use by prune growers of new rust management strategies depends on knowledge of infection events and therefore requires ready access to environmental data from orchards. To satisfy this need, microprocessor-based device called a Prune Rust Infection Predictor (PRIP) was developed. The instrument measures air temperature, rainfall, and the presence or absence of free water tree canopies. Measurements are taken at 5-min intervals during wet periods. The instrument calculates whether or not conditions have been suitable for rust infection and potential severity of infection. The system has been operated by advisory staff of NSW Agriculture Fisheries since the 1988/1989 growing season. Potential infection period and related environmental data are collected by personal computers via permanently installed telephone lines from PRIPs located within prune orchards. Prune growers are then advised by recorded telephone messages and given appropriate disease mangement advice. Cumulative potential infection period values are used to provide a prognosis of current-season rust epidemic severity. An expert system is also being developed to assist growers in making decisions on specific rust management options.     

Comparison of methods to assess dog owners' therapeutic compliance

Objective To compare different methods for assessing the compliance of veterinary clients administering medication to their dogs.
Procedure Thirty-one owners whose dogs were prescribed amoxycillin-clavulanate, twice and thrice daily, for a duration of five to seven days were recruited from three Sydney veterinary hospitals. Compliance was assessed by electronic monitoring devices, return medication counts, client self-reports and veterinarians' estimation of likely compliance.
Results Electronic monitoring showed owners administered on average 84% (range 7 to 104%) of prescribed medication to their dogs. Both return medication counts and client self-reports tended to overestimate therapeutic compliance when compared with electronic monitoring. When questioned, the majority of owners (71%) claimed perfect compliance with the prescribed regimen. No correlation was found between veterinarians' estimates of owner compliance and that assessed electronically.
Conclusion Electronic monitoring provided valuable information on dose timing and variation, but proved costly and difficult to employ in veterinary practice. Simplicity and low cost of return medication counts makes this method attractive for use in veterinary compliance studies. Client self-reports reliably detected some noncompliers and permitted identification of individual problems or errors. For practical purposes a combination of return medication counts and client self-reports may have merit in future veterinary compliance studies.     

Simulation study of the competitive ability of erect, semi-erect and prostrate cowpea (Vigna unguiculata) genotypes

Ecophysiological simulation models provide a quantitative method to predict the effects of management practices, plant characteristics and environmental factors on crop and weed growth and competition. The INTERCOM interplant competition model was parameterised, calibrated by monoculture data for three cowpea (Vigna unguiculata) genotypes that differed in growth habit, common sunflower (Helianthus annuus) and common purslane (Portulaca oleracea), and used to simulate competition of cowpea cover crops with sunflower or purslane. The simulation results were compared with observations from field competition experiments in 2003 and 2004. INTERCOM more accurately simulated actual field data for the competition of cowpea genotypes and sunflower than companion field experiments for the competition of cowpea and purslane. The validated simulation model of cowpea and sunflower at two densities was used to study the effects of cowpea growth habit on final biomass production of cowpea and sunflower. The model suggested that erect growth habit was more competitive than semi‐erect and prostrate growth habit, when cowpea genotypes were grown with sunflower. Cowpea leaf area distribution was important to higher cowpea biomass production, while cowpea height growth was important to reduce sunflower biomass. Our simulation approach is suggested as a method for crop breeders to gauge the likely success of selection for competitive crops before undertaking expensive long‐term breeding experiments.