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Paolo MARTELLI Serena OH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):809
A 13-year-old Bornean orangutan diagnosed with life threatening Streptococcus pyogenes broncho-pneumonia was kept in a state of deep sedation for 20 days via continuous intra-venous (IV) infusion of zolazepam -tiletamine and IV haloperidol to allow consistent IV administration of ceftazidime and gatifloxacine. The use of long-term deep sedation allowed carrying out a particularly demanding treatment not generally associated with zoological patients. The treatment was ultimately successful. 相似文献
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JEONGMIN LEE KIDONG EOM YUNSANG SEONG HAEOON LEE JINHEE PARK JONGWON LEE KWANGHO JANG KEUNWOO LEE TAEHO OH SANGGWAN LEE JUNGHEE YOON HEECHUN LEE HOJUNG CHOI YOUNWON LEE DONGWOO CHANG 《Veterinary radiology & ultrasound》2006,47(1):94-98
Ultrasonographic imaging of the canine external ear canal, tympanic membrane, and tympanic bulla was described in five healthy beagle dogs before and after infusion of saline into the ear canal. Saline served as an acoustic window. With this method, the external ear canal, and tympanic bulla were visible in the same imaging plane and the integrity of the tympanic membrane could be evaluated indirectly by confirming an intact tympanic membrane, which appeared at the end of the ear canal as a hyperechoic line with reverberation. Experimentally, perforated tympanic membrane could be evaluated by identifying anechoic saline in the tympanic bulla lumen. The air and fluid-filled tympanic bulla were also visualized. Ultrasonography with saline as an acoustic window appears to be helpful for the evaluation of the external ear canal, tympanic membrane, and tympanic bulla and it may have the potential to be a useful clinical tool in evaluation of integrity of the tympanic membrane. 相似文献
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Min‐Jung GU Md. Jahangir ALAM Seon‐Ho KIM Che‐Ok JEON Moon‐Baek CHANG Young‐Kyoon OH Sang‐Cheol LEE Sang‐Suk LEE 《Animal Science Journal》2011,82(5):663-672
Molecular diversity of methanogens in the rumen of Korean black goats was investigated with 16S rRNA gene clone libraries using methanogen‐specific primers. The libraries were composed of rumen fluid‐associated methanogens (FAM) and rumen particle‐associated methanogens (PAM) from rumen‐fistulated Korean black goats. Among the 141 clones of the FAM library, the sequences were mostly related to two phyla, the Methanobacteriaceae family (77.3%) and the Thermoplasmatales family (22.7%); and among the 68 clones of the PAM library, sequences were also mainly clustered in the two phyla, the Thermoplasmatales family (63.24%) and the Methanobacteriaceae family (35.29%). Most of the sequenced clones in the two libraries were closely related to uncultured methanogenic archaeon. Quantitative real‐time PCR revealed that PAM (8.97 log 10) had significantly higher (P < 0.01) density of methanogens by the methanogenic 16S rRNA gene copies than FAM (7.57 log 10). The two clone libraries also showed difference in Shannon index (FAM library 1.70 and PAM library 1.59) and Chao 1 estimator (FAM library 18 and PAM library 17 operational taxonomic units). Apparent differences found in the microbial community from the two 16S rRNA gene libraries could be a result of such factors as the chemical and physical nature of the target material surface, types or component of diets, the interaction between the methanogens and other microbes, and age of the experimental goats. 相似文献
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ABSTRACT Almond leaf scorch disease (ALSD) has recently reemerged in the San Joaquin Valley of California threatening almond production. ALSD is caused by Xylella fastidiosa, a nutritionally fastidious bacterium. Single nucleotide polymorphisms (SNPs) in the 16S rRNA gene (16S rDNA) of X. fastidiosa strains were identified to characterize the bacterial population in infected trees. Genotype-specific SNPs were used to design primers for multiplex polymerase chain reaction assays of early passage cultures. Two genotypically distinct types of X. fastidiosa strains, G-type and A-type, coexist simultaneously in the same infected almond orchard. This was substantiated by restriction fragment length polymorphism analysis of a different genetic locus, RST31-RST33, which has previously been used to identify and differentiate X. fastidiosa strains. Furthermore, unique bacterial colony morphology was consistently associated with the A-type X. fastidiosa strains. To our knowledge, this is the first report of a mixed genotype infection of X. fastidiosa disease on the same location under natural environmental conditions. The concept of mixed genotype infection could affect the current epidemiological study based on the assumption that one genotype causes ALSD on one location and, therefore, the disease management strategy. 相似文献
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醋糟菌糠对3种作物土壤微生物及酶活性的影响 总被引:2,自引:0,他引:2
为研究醋糟菌糠作为一种生物肥料对土壤肥力的影响,为解决食用菌菌糠对环境污染及其有效利用提供科学依据,本文通过田间试验,对玉米、高粱、糯玉米3种作物基施醋糟菌糠后不同生育期的土壤脲酶、蔗糖酶和过氧化氢酶活性,细菌、放线菌和真菌数量,及微生物量碳、氮含量进行测试。研究结果表明:1)醋糟菌糠显著提高了作物根际土壤细菌、放线菌和真菌的数量。在作物整个生育期,加入醋糟菌糠的土壤细菌数量比对照提高32%~54%;放线菌数量在成熟期提高明显,玉米田土壤放线菌数量增幅最大,为101%;真菌数量变化总体趋势呈现先升高后下降的趋势。2)醋糟菌糠的施入增加了作物根际土壤脲酶、过氧化氢酶、蔗糖酶的活性。种植高粱、玉米和糯玉米土壤的脲酶活性增长率分别为239%、189%和185%;3种作物的土壤过氧化氢酶活性在抽穗期最高,最大增长率为40%;3种作物的土壤蔗糖酶活性在不同生育期变化趋势不同,玉米各生育期土壤蔗糖酶活性变化较为稳定,其增长率分别为38%、28%、48%。3)醋糟菌糠的施入增加了作物根际土壤微生物碳氮的含量,不同生育期3种作物的土壤微生物碳含量增幅为58.10~407.67 mg·kg-1,微生物氮含量增幅为11.98~27.55 mg·kg-1。由此可见,醋糟菌糠的施用可以增强土壤生产力的可持续性,从而达到保护和改善土壤环境的效果。同时该研究也为醋糟菌糠的有效利用提供了一定的科学依据。 相似文献
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Simple method for extracting the seasonal signals of photochemical reflectance index and normalized difference vegetation index measured using a spectral reflectance sensor
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A spectral reflectance sensor(SRS) fixed on the near-surface ground was developed to support the continuous monitoring of vegetation indices such as the normalized difference vegetation index(NDVI) and photochemical reflectance index(PRI). NDVI is useful for indicating crop growth/phenology, whereas PRI was developed for observing physiological conditions. Thus, the seasonal change patterns of NDVI and PRI are two valuable pieces of information in a crop-monitoring system. However, capturing the seasonal patterns is considered challenging because the vegetation index values estimated by the reflection from vegetation are often governed by meteorological conditions, such as solar irradiance and precipitation. Further, unlike growth/phenology, the physiological condition has diurnal changes as well as seasonal characteristics. This study proposed a novel filtering method for extracting the seasonal signals of SRS-based NDVI and PRI in paddy rice, barley, and garlic. First, the measurement accuracy of SRSs was compared with handheld spectrometers, and the R~2 values between the two devices were 0.96 and 0.81 for NDVI and PRI, respectively. Second, the experimental study of threshold criteria with respect to meteorological variables(i.e., insolation, cloudiness, sunshine duration, and precipitation) was conducted, and sunshine duration was the most useful one for excluding distorted values of the vegetation indices. After data processing based on sunshine duration, the R2 values between the measured vegetation indices and the extracted seasonal signals of vegetation indices increased by approximately 0.002–0.004(NDVI) and 0.065–0.298(PRI) on the three crops, and the seasonal signals of vegetation indices became noticeably improved. This method will contribute to an agricultural monitoring system by identifying the seasonal changes in crop growth and physiological conditions. 相似文献
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Efficacy assessment of an intramammary treatment with a new recrystallized enrofloxacin vs ceftiofur and parenteral enrofloxacin in dairy cows with nonsevere clinical mastitis
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M. Viveros R. Lopez‐Ordaz L. Gutiérrez J. E. Miranda‐Calderón H. Sumano 《Journal of veterinary pharmacology and therapeutics》2018,41(1):e1-e9
A recrystallized form of enrofloxacin as dehydrate‐HCl (enro‐C) was assessed for bacteriological and clinical cure efficacies in Holstein‐Friesian cows affected of nonsevere clinical mastitis. Treatments were enro‐Csusp (n = 81), treated with a pharmaceutical suspension of enro‐C/quarter; group enro‐Cpd (n = 80) treated as above, but using enro‐C powder suspended in water; group CF (n = 65), treated with ceftiofur HCl/quarter; and group enroR (n = 66), treated with standard enrofloxacin solution (5 mg/kg, intramuscular). Cows had a mean milk production of 31 L/day and were 2‐3 lactational periods old. Treatments were administered every 24 hr for 3 days. Groups treated with enro‐C exhibited statistically significant (p > .05) better clinical cure as compared to groups treated with CF or enroR (95.06%, 96.25%, 67.79%, and 57.55%, for enro‐Csusp, enro‐Cpd, CF, and enroR, respectively). In contrast, probability of bacteriological cure was not statistically different among treatments. Yet, the outstanding clinical and bacteriological cure rates obtained for enro‐C for nonsevere cases of mastitis is superior to previously reported data for parenteral enrofloxacin and other antibacterial‐intramammary treatments. Impact of using enro‐C on the rate and pattern of bacterial resistance, somatic cell counts and milk electric conductivity, must be studied. Also, the use of enro‐C for complicated cases of mastitis should be studied and milk withdrawal times must be accurately established. 相似文献