Identifying crop yield gaps with site- and season-specific data-driven models of yield potential

Precision Agriculture - There is considerable interest and value in identifying the gap between crop yields that have actually been achieved, and yields that could have potentially been achieved. A...     

Molecular characerization of the interaction between the fungal pathogen Cladosporium fulvum and tomato

The fungus Cladosprium fulvum is a biotrophic leaf pathogen of tomato. The fungus develops in the intercellular space without forming specialized feeding structures and does not affect the leaf tissue. The outcome of the C. fulvum-tomato interaction can be described by the gene-for-gene model. Failure of infection is expressed by a hypersensitive response. Two fungal proteins, ECP1 and ECP2, have been isolated and their corresponding genes have been cloned. In a compatible interaction including many physiological races ECP1 and ECP2 are highly produced and a role in pathogenicity is suggestive. The ecp1 gene shows some homology with tumor necrosis factor receptors (TNFRs) while the ecp2 gene shows no homology with sequences known in data bases. However, disruption of one of the two genes showed no reduced pathogenicity of the fungus. Two race-specific elicitors, AVR4 and AVR9, have been isolated and their corresponding genes have been cloned. The avirulence genes Avr4 and Avr9 are only present in C. fulvum avirulent on Cf-4 and Cf-9 cultivars, respectively. The expression of these two genes is, like the expression of the ecp genes, highly induced when the fungus grows in planta. Disruption of the Avr9 gene in wild type avirulent races leads to virulence on tomato genotypes carrying the complementary resistance gene Cf-9. A single base-pair change in the avirulence gene Avr4 leads to virulence on tomato genotypes carrying the Cf-4 resistance gene. Isolation, characterization and possible function of ECP1, ECP2, AVR4, and AVR9 will be discussed.     

Hydrological impacts of rainwater harvesting (RWH) in a case study catchment: The Arvari River, Rajasthan, India. Part 1: Field-scale impacts

Rainwater harvesting (RWH), the small-scale collection and storage of runoff to augment groundwater stores, has been seen as a solution to the deepening groundwater crisis in India. However, hydrological impacts of RWH in India are not well understood, particularly at the larger catchment-scale. A key element to grasping RWH impact involves understanding the generated recharge variability in time and space, which is the result of variability in rainfall-runoff and efficiency of RWH structures. Yet there are very few reported empirical studies of the impact of RWH. Catchment-scale impacts are best studied using a water balance model, which would require a basic level of field data and understanding of the variability. This study reports the results of a 2-year field study in the 476 km² semi-arid Arvari River catchment, where over 366 RWH structures have been built since 1985. Difficulties associated with working in semi-arid regions include data scarcity. Potential recharge estimates from seven RWH storages, across three different types and in six landscape positions, were calculated using the water balance method. These estimates were compared with recharge estimates from monitored water levels in 29 dug wells using the water table fluctuation method. The average daily potential recharge from RWH structures varied between 12 and 52 mm/day, while estimated actual recharge reaching the groundwater ranged from 3 to 7 mm/day. The large difference between recharge estimates could be explained through soil storage, local groundwater mounding beneath structures and a large lateral transmissivity in the aquifer. Overall, approximately 7% of rainfall is recharged by RWH in the catchment, which was similar in the comparatively wet and dry years of the field analysis. There were key differences between RWH structures, due to engineering design and location. These results indicate that recharge from RWH affects the local groundwater table, but also has potential to move laterally and impact surrounding areas. However, the greatest weakness in such analysis is the lack of information available on aquifer characteristics, in addition to geology and soil type.     

Identification and localization of the structural proteins of anguillid herpesvirus 1

ABSTRACT: Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla. Despite their importance, fundamental molecular knowledge on fish herpesviruses is still limited. In this study we describe the identification and localization of the structural proteins of AngHV-1. Purified virions were fractionated into a capsid-tegument and an envelope fraction, and premature capsids were isolated from infected cells. Proteins were extracted by different methods and identified by mass spectrometry. A total of 40 structural proteins were identified, of which 7 could be assigned to the capsid, 11 to the envelope, and 22 to the tegument. The identification and localization of these proteins allowed functional predictions. Our findings include the identification of the putative capsid triplex protein 1, the predominant tegument protein, and the major antigenic envelope proteins. Eighteen of the 40 AngHV-1 structural proteins had sequence homologues in related Cyprinid herpesvirus 3 (CyHV-3). Conservation of fish herpesvirus structural genes seemed to be high for the capsid proteins, limited for the tegument proteins, and low for the envelope proteins. The identification and localization of the structural proteins of AngHV-1 in this study adds to the fundamental knowledge of members of the Alloherpesviridae family, especially of the Cyprinivirus genus.     

Expanded repeat in canine epilepsy

Epilepsy afflicts 1% of humans and 5% of dogs. We report a canine epilepsy mutation and evidence for the existence of repeat-expansion disease outside humans. A canid-specific unstable dodecamer repeat in the Epm2b (Nhlrc1) gene recurrently expands, causing a fatal epilepsy and contributing to the high incidence of canine epilepsy. Tracing the repeat origins revealed two successive events, starting 50 million years ago, unique to canid evolution. A genetic test, presented here, will allow carrier and presymptomatic diagnosis and disease eradication. Clinicopathologic characterization establishes affected animals as a model for Lafora disease, the most severe teenage-onset human epilepsy.     

A positive test of East Antarctica-Laurentia juxtaposition within the Rodinia supercontinent

The positions of Laurentia and other landmasses in the Precambrian supercontinent of Rodinia are controversial. Although geological and isotopic data support an East Antarctic fit with western Laurentia, alternative reconstructions favor the juxtaposition of Australia, Siberia, or South China. New geologic, age, and isotopic data provide a positive test of the juxtaposition with East Antarctica: Neodymium isotopes of Neoproterozoic rift-margin strata are similar; hafnium isotopes of approximately 1.4-billion-year-old Antarctic-margin detrital zircons match those in Laurentian granites of similar age; and a glacial clast of A-type granite has a uraniun-lead zircon age of approximately 1440 million years, an epsilon-hafnium initial value of +7, and an epsilon-neodymium initial value of +4. These tracers indicate the presence of granites in East Antarctica having the same age, geochemical properties, and isotopic signatures as the distinctive granites in Laurentia.     

Antibiotic sensitivity of Pasteurella haemolytica isolated by means of a fibreoptic endoscope from cases of pneumonic pasteurellosis in cattle

Bacterial isolations from tracheal and bronchial washes obtained with the aid of a fibreoptic endoscope were carried out over a 7 month period in a feedlot on calves suffering from acute pneumonic pasteurellosis. Pasteurella haemolytica and Pseudomonas aeruginosa represented the majority of isolates. Antibiotic sensitivities of the Pasteurella isolates are reported on.     

Historical Milestones,Current Methods,and Strategies Resulting in Year-Round Strawberry Production in Belgium

ABSTRACT

Strawberry production in Belgium has evolved from being a June-harvested crop grown in soil to a year-round crop grown in multiple production systems, as a result of the development of new cultivars and production techniques. Strawberry cultivation used to be one aspect of diverse family enterprises, but now strawberry farms are specialized. In the beginning, strawberries were only harvested in June. Covering the crop with plastic tunnels allowed the harvest to advance to May. Subsequently, an important innovation was the use of cold-stored plants in year-round plantings, which also allowed later production. This evolution in combination with the development of various cultivation systems using peat substrates (including table-tops, plastic greenhouses, and glasshouses) resulted in a strawberry production period from March until December–January. Recently, interest in assimilation lighting in glasshouses has increased. At first, this allowed advanced production in February, but now even winter production is possible, resulting in fresh Belgian strawberries 365 days a year.     

AGTR2 mutations in X-linked mental retardation

Two angiotensin II (Ang II)-specific receptors, AGTR1 and AGTR2, are expressed in the mammalian brain. Ang II actions on blood pressure regulation, water electrolyte balance, and hormone secretion are primarily mediated by AGTR1. The function of AGTR2 remains unclear. Here, we show that expression of the AGTR2 gene was absent in a female patient with mental retardation (MR) who had a balanced X;7 chromosomal translocation. Additionally, 8 of 590 unrelated male patients with MR were found to have sequence changes in the AGTR2 gene, including one frameshift and three missense mutations. These findings indicate a role for AGTR2 in brain development and cognitive function.