排序方式: 共有16条查询结果,搜索用时 15 毫秒
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Wang Z Shen D Parsons DW Bardelli A Sager J Szabo S Ptak J Silliman N Peters BA van der Heijden MS Parmigiani G Yan H Wang TL Riggins G Powell SM Willson JK Markowitz S Kinzler KW Vogelstein B Velculescu VE 《Science (New York, N.Y.)》2004,304(5674):1164-1166
Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine phosphatase gene superfamily in human cancers identified 83 somatic mutations in six PTPs (PTPRF, PTPRG, PTPRT, PTPN3, PTPN13, PTPN14), affecting 26% of colorectal cancers and a smaller fraction of lung, breast, and gastric cancers. Fifteen mutations were nonsense, frameshift, or splice-site alterations predicted to result in truncated proteins lacking phosphatase activity. Five missense mutations in the most commonly altered PTP (PTPRT) were biochemically examined and found to reduce phosphatase activity. Expression of wild-type but not a mutant PTPRT in human cancer cells inhibited cell growth. These observations suggest that the mutated tyrosine phosphatases are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention. 相似文献
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Sjöblom T Jones S Wood LD Parsons DW Lin J Barber TD Mandelker D Leary RJ Ptak J Silliman N Szabo S Buckhaults P Farrell C Meeh P Markowitz SD Willis J Dawson D Willson JK Gazdar AF Hartigan J Wu L Liu C Parmigiani G Park BH Bachman KE Papadopoulos N Vogelstein B Kinzler KW Velculescu VE 《Science (New York, N.Y.)》2006,314(5797):268-274
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Participation of phosphofructokinase,malate dehydrogenase and isocitrate dehydrogenase in capacitation and acrosome reaction of boar spermatozoa
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E Breininger D Dubois VE Pereyra PC Rodriguez MM Satorre PD Cetica 《Reproduction in domestic animals》2017,52(5):731-740
The aim of this work was to determine the enzymatic activity of phosphofructokinase (PFK), malate dehydrogenase (MDH) and isocitrate dehydrogenase (IDH) in boar spermatozoa and study their participation in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction. Enzymatic activity of these enzymes was determined spectrophotometrically in extracts of boar spermatozoa. Sperm suspensions were incubated in the presence of bicarbonate (40 mM), a well‐known capacitation inducer, or follicular fluid (30%), as an acrosome reaction inducer, and different concentrations of oxoglutarate, oxalomalate and hydroxymalonate, inhibitors of PFK, IDH and MDH, respectively. Capacitation percentages were determined by the fluorescence technique of chlortetracycline (CTC), and true acrosome reaction was determined by trypan blue and differential–interferential contrast, optical microscopy. The activity of PFK in boar spermatozoa enzymatic extracts was 1.70 ± 0.19 U/1010 spermatozoa, the activity of NAD‐ and NADP‐dependent IDH was 0.111 ± 0.005 U/1010 and 2.22 ± 0.14 U/1010 spermatozoa, respectively, and the activity of MDH was 4.24 ± 0.38 U/1010 spermatozoa. The addition of the specific inhibitors of these enzymes prevented sperm capacitation and decreased sperm motility during capacitation and inhibited the acrosome reaction (AR), without affecting the sperm motility during this process. Our results demonstrate the participation of PFK, IDH and MDH in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction in boar spermatozoa, contributing to elucidate the mechanisms that produce energy necessary for these processes in porcine spermatozoa. 相似文献
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Bettegowda C Agrawal N Jiao Y Sausen M Wood LD Hruban RH Rodriguez FJ Cahill DP McLendon R Riggins G Velculescu VE Oba-Shinjo SM Marie SK Vogelstein B Bigner D Yan H Papadopoulos N Kinzler KW 《Science (New York, N.Y.)》2011,333(6048):1453-1455
Oligodendrogliomas are the second most common malignant brain tumor in adults and exhibit characteristic losses of chromosomes 1p and 19q. To identify the molecular genetic basis for this alteration, we performed exomic sequencing of seven tumors. Among other changes, we found that the CIC gene (homolog of the Drosophila gene capicua) on chromosome 19q was somatically mutated in six cases and that the FUBP1 gene [encoding far-upstream element (FUSE) binding protein] on chromosome 1p was somatically mutated in two tumors. Examination of 27 additional oligodendrogliomas revealed 12 and 3 more tumors with mutations of CIC and FUBP1, respectively, 58% of which were predicted to result in truncations of the encoded proteins. These results suggest a critical role for these genes in the biology and pathology of oligodendrocytes. 相似文献
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The Influence of Maca (<Emphasis Type="Italic">Lepidium meyenii</Emphasis>) on Antioxidant Status,Lipid and Glucose Metabolism in Rat 总被引:1,自引:0,他引:1
Vecera R Orolin J Skottová N Kazdová L Oliyarnik O Ulrichová J Simánek V 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(2):59-63
This work focused on the effect of maca on lipid, anti-oxidative, and glucose parameters in hereditary hypertriglyceridemic
(HHTg) rat. Maca (1%) was administred to rats as a part of a high-sucrose diet (HSD) for 2 weeks. Rosiglitazone (0.02%) was
used as a positive control. Maca significantly decreased the levels of VLDL (very low density lipoproteins), LDL (low density
lipoproteins), and total cholesterol, and also the level of TAG (triacylglycerols) in the plasma, VLDL, and liver. Maca, as
well as rosiglitazone, significantly improved glucose tolerance, as the decrease of AUC (area under the curve) of glucose
showed, and lowered levels of glucose in blood. The activity of SOD (superoxide dismutase) in the liver, the GPX (glutathione
peroxidase) in the blood, and the level of GSH (glutathione) in liver increased in all cases significantly. Results demonstrate
that maca seems to be promising for a positive influence on chronic human diseases (characterized by atherogenous lipoprotein
profile, aggravated antioxidative status, and impaired glucose tolerance), and their prevention. 相似文献