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Detection and Quantification of Phytophthora ramorum from California Forests Using a Real-Time Polymerase Chain Reaction Assay 总被引:1,自引:0,他引:1
ABSTRACT The timely and accurate detection of pathogens is a critical aid in the study of the epidemiology and biology of plant diseases. In the case of regulated organisms, the availability of a sensitive and reliable assay is essential when trying to achieve early detection of the pathogen. We developed and tested a real-time, nested polymerase chain reaction (PCR) assay for the detection of Phytophthora ramorum, causal agent of sudden oak death. This technique then was implemented as part of a widespread environmental screen throughout California. The method here described is sensitive, detecting less than 12 fg of pathogen DNA, and is specific for P. ramorum when tested across 21 Phytophthora spp. Hundreds of symptomatic samples from 33 sites in 14 California counties were assayed, resulting in the discovery of 10 new host species and 23 infested areas, including 4 new counties. With the exception of a single host, PCR-based discovery of new hosts and infested areas always was confirmed by traditional pathogen isolations and inoculation studies. Nonetheless, molecular diagnostics were key in early pathogen detection, and steered the direction of further research on this newly discovered and generalist Phytophthora species. 相似文献
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Tien‐Chun Wan Yu‐Tse Liu Lan‐Tyi Duann Kuo‐Hui Yu Chih‐Ming Chen Liang‐Chuan Lin Ryoichi Sakata 《Animal Science Journal》2014,85(1):75-80
The purposes of this study were to assess the improvement of fatty liver induced by ethanol with animal liver and bile extracts. This research aimed to increase the economic values of animal liver and bile extracts and used these to reduce damage of ethanol‐induced fatty liver. Extracts came from animal liver and bile, including pig bile powder, pig liver extract, a mixture of pig bile powder and pig liver extract, chicken bile powder, chicken liver extract, and a mixture of chicken bile powder and chicken liver extract, and these were fed to Long‐Evans rats. The results showed that rats fed ethanol for long terms could increase values of aspartate transaminase, cholesterol, γ‐glutamy‐transferase and alkaline phosphatase. Pig bile powder could decrease the values of aspartate transaminase, cholesterol and γ‐glutamy‐transferase. The significances also decreased on aspartate transaminase, γ‐glutamy‐transferase and aspartate transaminase, which were carried out with the pig liver extract treatment. These results suggest pig bile and liver extracts have high potential to improve rats' ethanol‐induced fatty liver with serum biochemical parameters. 相似文献
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The metallization of hydrogen directly would require pressure in excess of 400 gigapascals (GPa), out of the reach of present experimental techniques. The dense group IVa hydrides attract considerable attention because hydrogen in these compounds is chemically precompressed and a metallic state is expected to be achievable at experimentally accessible pressures. We report the transformation of insulating molecular silane to a metal at 50 GPa, becoming superconducting at a transition temperature of Tc = 17 kelvin at 96 and 120 GPa. The metallic phase has a hexagonal close-packed structure with a high density of atomic hydrogen, creating a three-dimensional conducting network. These experimental findings support the idea of modeling metallic hydrogen with hydrogen-rich alloy. 相似文献
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Evaluation of a screening programme for identification of mannosidosis heterozygotes in angus cattle
R.D. Jolly B.V.Sc Ph.D. M.A.C.V.Sc. K.G. Thompson B.V.Sc C.A. Tse B.Sc. 《New Zealand veterinary journal》2013,61(10):185-190
Sir, — In recent years, much research effort has been directed towards identifying the cause of Ryegrass staggers (RGS). Many promising leads have been investigated, including studies of fungi in soil, ryegrass leaves, and dead leaf litter that could produce tremorgenic mycotoxins(3). Several groups of fungi produce tremorgens(4) which, when injected into sheep, produce symptoms similar to those in field outbreaks of RGS. However, under field conditions, populations of these fungi have not been shown to differ significantly between toxic and non-toxic pasture(7) (McKenzie &; Byford, unpublished data). 相似文献
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Tien‐Chun WAN Fu‐Yuan CHENG Yu‐Tse LIU Chao‐Ming WANG Ching‐Lin SHYU Chih‐Ming CHEN Liang‐Chuan LIN Ryoichi SAKATA 《Animal Science Journal》2008,79(6):693-698
A novel Bacillus species of Calculus Bovis (cow gallstone) was isolated and identified in this study. Morphological features, bacterial fatty acid analysis using a microbial identification system, carbon source utilization profiles using Biolog system and 16S rDNA sequencing were employed to identify the isolated bacterium. This isolated bacterium was observed to be Gram‐negative, aerobic growing, rod‐shaped and short chain. The results of bacterial fatty acid analysis and physiological characteristics were not matched to the database. The main fatty acids found in the bacterium were 65.96% branched chain saturated fatty acids (iso C11:0, anteiso C11:0 and iso C13:0~anteiso C19:0). The bacterium oxidized 35 carbon sources and weakly responded with 49 of the 95 different carbon sources analyzed with the Biolog identification system. Based on 16S rDNA sequence analysis, this bacterium was classified as a novel Bacillus species. 相似文献
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Chui Ching Wong Su Ming Thean Youming Ng Joanne Su Lin Kang Tong Yong Ng Man Ling Chau Tse Hsien Koh Kwai Peng Chan 《Zoonoses and public health》2019,66(7):773-782
Hepatitis E virus (HEV) causes 20 million infections worldwide yearly, of which only about 3.3 million are symptomatic. In developed Asian countries, HEV strains detected in human sera and in food sources were genetically similar, suggesting that indigenous HEV infections may be largely food‐borne. To assess the burden of hepatitis E in Singapore, we performed a seroepidemiologic study of the infection. Additionally, we carried out HEV genotyping on archived, residual HEV IgM‐positive serum samples collected between 2014 and 2016 (n = 449), and on pig liver samples (n = 36) purchased from wet markets and supermarkets. Our study shows a rise in hepatitis E incidence (IgM) from 1.7 to 4.1 cases per 100,000 resident population from 2012 to 2016 and an increase in hepatitis E IgG positivity rate among residents from 14% in 2007 to 35% in 2016. Other findings also suggest the epidemiology of hepatitis E in Singapore has shifted, from it being mainly a disease imported from the Indian subcontinent, to one that is now increasingly prevalent in our resident population. Genotypes obtained from 143 human samples identified the majority to be genotype 3 (n = 121), 21 to be genotype 1 and one to be genotype 4. Further phylogenetic analyses suggest genotype 3a to be the cause of indigenous infections in residents, which showed genetic similarity to the genotype 3a strains detected in pig livers. This link between the strains in the majority of human samples and those in pig livers consumed by the public suggests a possible food‐borne route of HEV infection in Singapore. 相似文献
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In this report, we describe an investigation into the cause of chronic inflammation in a clinically normal 5-year-old racing Thoroughbred, finally confirmed to be associated with B-cell lymphoma. Over a 14-week period, the horse was regularly evaluated by sequential blood testing, revealing persistent inflammation of unknown origin, unresponsive to treatment. Eventually, an abdominal mass was identified by transabdominal ultrasound and rectal palpation. Surgical exploration revealed an extensive, invasive soft tissue mass in the abdomen. At post-mortem examination, a mass involving the colon, mesocolon and mesocolonic lymph node was sampled and confirmed as a large B-cell lymphoma by histopathology and immunohistochemistry. In cases of unexplained chronic inflammation in a horse, neoplasia should be considered as a possible aetiology. 相似文献
10.
The pathogenesis of paraesophageal empyema in dogs and constancy of radiographic and computed tomography signs are linked to involvement of the mediastinal serous cavity 下载免费PDF全文
Karine Gendron Sean P. McDonough James A. Flanders May Tse Peter V. Scrivani 《Veterinary radiology & ultrasound》2018,59(2):169-179
The mediastinal serous cavity is a normal anatomic space in the caudal mediastinum. Aims of this anatomic and case series study were to describe the signs of pathologic expansion of the mediastinal serous cavity observed during computed tomography (CT), review the underlying anatomy, perform a literature review, and evaluate the medical records of several dogs with mediastinal serous cavity empyema (paraesophageal empyema). The mesothelial lined mediastinal serous cavity is a cranial extension of the omental bursa, separated from the peritoneal cavity by the diaphragm, in the dorsal part of the caudal mediastinum, to the right of the esophagus, between the heart base and diaphragm. In five adult, large‐breed dogs with surgically and histologically confirmed paraesophageal empyema, macroscopic plant material was found at surgery in two dogs, adherence to adjacent lung was present in three different dogs, accessory lobectomy was performed in two dogs with subacute‐chronic pyogranulomatous pneumonia, and one dog had concurrent pyothorax and mediastinitis, but none had esophageal abnormalities. This study expands our understanding of the pathogenesis and basis for the imaging appearance of paraesophageal empyema in dogs by clarifying the underlying anatomic structures that direct development of this condition. The term empyema accurately describes this condition because the purulent material accumulates within an existing body cavity. The study also provides initial evidence that the development of paraesophageal empyema might be due to local extension of lung disease, such as foreign body migration or pneumonia. Computed tomography was helpful for diagnosis, assessing size, and determining the spread of disease. 相似文献