鸡传染性鼻炎流行病学调查（Ⅱ）

利用鸡传染性鼻炎PCR试剂盒和阻断ELISA试剂盒，对29份菌株、136份临床样品和1019份血清进行了检验和分析，结果PCR阳性率为36.4%，A型阳性率为10.3%，C型阳性率为6.4%。     

RTDrift: A real time model for estimating spray drift from ground applications

A spray drift model called RTDrift was developed to estimate drift caused by ground sprayer machines. The sprayer was equipped with sensors measuring operational parameters namely spray pressure, boom height and movements, and geolocalisation. Climatic parameters, including wind speed and direction, were measured using a 2-D ultrasonic anemometer mounted on the sprayer. The nozzles spray drop size spectra were characterised using Phase Doppler Interferometer measurements. At every successive boom position, a diffusion-advection Gaussian tilted plume model computed the spray drift deposits for each drop class taking into account evaporation. The contribution of a single nozzle was calculated by integration of the individual puffs with respect to time and summation of the contributions of individual drops classes. The overall drift generated by the sprayer machine was obtained adding the contributions of all the nozzles. Field trials were performed on a fallow field with water and on crops with pesticides in various wind conditions. The ground drift was measured at different drift distances using fluorometric methods. When comparing the results of the model with experimental measurements of deposits, the model produced realistic maps of drift deposits. Some further improvement is needed in the presence of large scale eddies. The model offers potential benefits for the farmer as a real time drift estimator embedded on a sprayer machine.     

A cellular framework for gut-looping morphogenesis in zebrafish

Many vertebrate organs adopt asymmetric positions with respect to the midline, but little is known about the cellular changes and tissue movements that occur downstream of left-right gene expression to produce this asymmetry. Here, we provide evidence that the looping of the zebrafish gut results from the asymmetric migration of the neighboring lateral plate mesoderm (LPM). Mutations that disrupt the epithelial structure of the LPM perturb this asymmetric migration and inhibit gut looping. Asymmetric LPM migration still occurs when the endoderm is ablated from the gut-looping region, suggesting that the LPM can autonomously provide a motive force for gut displacement. Finally, reducing left-sided Nodal activity randomizes the pattern of LPM migration and gut looping. These results reveal a cellular framework for the regulation of organ laterality by asymmetrically expressed genes.     

Droplet size spectra and drift effect of two phenmedipham formulations and four adjuvants mixtures

The effect of 4 adjuvants on two formulations of phenmedipham (EC and SC) applied with three types of hydraulic nozzles (hollow cone, flat fan and air induction) was evaluated using a wind tunnel to determine spray drift potential (DP). The volume median diameter (VMD) and the percentage by volume of droplets less than 100 μm diameter was determined using a Malvern particle size analyser. In a wind tunnel DP was measured between 2 and 6 m downwind of the nozzle on glass fibre collectors using a fluorescent tracer. The EC formulation produced a smaller VMD and larger DP than the SC formulation irrespective of any effect due to adjuvants. DP increased as the proportion of small droplets increased.     

Comparison of External and Internal Pelvic Measurements of Belgian Blue Cattle from Sample Herds in Belgium and the United Kingdom

Measurements describing pelvic conformation of pedigree Belgian Blue cows were obtained from a sample of nine herds in Flanders, Belgium, comprising 111 adult breeding cows, and from 11 herds in the United Kingdom comprising 108 similar cows. All herds in the Belgian sample (111 cows) managed parturition by elective caesarian section as did seven herds (56 cows) in the UK sample; the remainder of UK herds (52 cows) allowed cows the opportunity to calve naturally per vagina before resorting to caesarian section. The data described the external and internal measurements, and pelvic area characteristic of this breed, irrespective of the different selection pressures applied over a number of years to this breed through variation in farm management and market forces present in either country. From these data, generalized linear models were constructed to predict pelvic area; they correctly identified cows with either small or large pelvic areas. There were no significant differences in pelvic conformation between cattle bred either in Belgium or the UK, although those cows bred in herds where natural calving was allowed to take place had significantly larger internal pelvic height (p < 0.05) and area (p < 0.05) than other Belgian Blue cows. The correlation coefficients between internal pelvic height and width and external pelvic measurements were significant (p < 0.001). These results might facilitate the selection of breeding cows with larger pelvic area so that a higher proportion of cows can calve naturally than currently occurs. However, selection for other traits such as relatively low birth weight combined with higher weaning weight should be carried out at the same time.     

No organ left behind: tales of gut development and evolution

The function of an organ is dependent on its cellular constituents as well as on their assembly into a cohesive unit. The developing gut faces unique challenges as one of the longest and largest organs in the body and also because it is constantly interfacing with external factors through the diet. Its location deep within the body has until recently hampered investigation into its formation. The patterning of the gut along its longitudinal, dorsoventral, left-right, and radial axes is one of the fascinating issues that pertain to the development, function, and homeostasis of this understudied organ.     

Clavibacter michiganensis ssp. insidiosus in lucerne seeds

Bacterial wilt is a serious disease of Iucerne. Although there are several ways to transmit the pathogen, seed transmission is practically the only way to introduce the bacteria to previously free areas. In this work, naturally infected seed material and commercial seed lots have been screened by a combination of immunofluorescence (IF) staining and dilution plating. Isolates were confirmed by biochemical characterization and finally by artificial infection of lucerne seedlings. For seed lots free from the pathogen, a diagnosis can mostly be achieved within 2 days. The culture step is necessary only if IF staining is not unequivocally negative, and the full procedure has to be run only for a small portion of the samples. The proposed seed assay provides a reliable alternative to the visual inspection of lucerne fields to fulfil the phytosanitary requirements for Clavibacter michiganensis ssp. insidiosus.     

动物疫苗及其合理使用（一）

疫苗接种是预防动物传染病发生的重要举措。依据免疫学理论,疫苗作为抗原,通过激发动物机体免疫系统,产生特异的体液免疫和细胞免疫,从而获得针对某种疾病的特异性免疫。疫苗一般分为细菌疫苗、病毒疫苗和类毒素疫苗等。用于防病治病的各种疫苗、菌苗又是特殊的生物药品,与普通化学药品具有完全不同的成分、功能和特性,疫苗中含有多种蛋白质（除细菌或病毒外,有的还含有保护剂、鸡胚尿囊液或细胞培养液）,它们均适合许多病原体的生长,有些制品还是活的微生物,一旦污染后果不堪设想。疫苗在保存、运输和使用时有其特别要求。如果方法不当,不但会使活性物质失活,起不到免疫防病作用;而且还可能适得其反,引发动物新的疾病,造成重大损失。