为明确辣蓼黄酮正丁醇部分(n-butanol part of flavonoids from Polygonum hydropiper L.,FNB)体外抗猪繁殖与呼吸综合征病毒(PPRSV)的效果。本研究以Marc-145细胞和PPRSV弱毒疫苗毒株(TJM-F92)为对象,通过CCK-8法检测FNB对细胞的毒性作用,并检测先给药后接毒、先接毒后给药、药物与病毒同时作用这3种方式处理细胞后药物对病毒的抑制率。结果发现,FNB对细胞的最大安全浓度为500 μg/mL,因此,选择25~500 μg/mL浓度范围的FNB进行后续试验。各浓度的FNB处理病毒后,能不同程度的抑制PRRSV在细胞上的增殖,并呈现一定的剂量效应关系,药物的浓度越高,抗病毒效果越好。其中,先接毒后给药、药物与病毒同时作用这两种方式抗PRRSV效果显著,在25~500 μg/mL浓度范围内细胞存活率分别为21.55%~65.23%和24.85%~73.60%。而先给药后接毒,不能有效降低病毒的感染力,在药物最高剂量(500 μg/mL)时细胞存活率仅为7.00%,抗病毒效果不明显。FNB预先作用于Marc-145细胞虽未降低PRRSV感染细胞的能力,即药物对于PRRSV预防作用效果不理想,但是FNB对病毒感染细胞后呈现一定的作用,药物能够通过抑制病毒的合成、释放及直接杀灭病毒,进而能够有效抑制PRRSV在细胞上的增殖。本试验结果不仅为FNB在临床上治疗猪繁殖与呼吸综合征(PRRS)提供参考依据,而且可以为辣蓼的深度开发和利用提供理论依据。 相似文献
Citrus fruits do not ripen at the same time in natural environments and exhibit different maturity stages on trees, hence it is necessary to realize selective harvesting of citrus picking robots. The visual attention mechanism reveals a physiological phenomenon that human eyes usually focus on a region that is salient from its surround. The degree to which a region contrasts with its surround is called visual saliency. This study proposes a novel citrus fruit maturity method combining visual saliency and convolutional neural networks to identify three maturity levels of citrus fruits. The proposed method is divided into two stages: the detection of citrus fruits on trees and the detection of fruit maturity. In stage one, the object detection network YOLOv5 was used to identify the citrus fruits in the image. In stage two, a visual saliency detection algorithm was improved and generated saliency maps of the fruits; The information of RGB images and the saliency maps were combined to determine the fruit maturity class using 4-channel ResNet34 network. The comparison experiments were conducted around the proposed method and the common RGB-based machine learning and deep learning methods. The experimental results show that the proposed method yields an accuracy of 95.07%, which is higher than the best RGB-based CNN model, VGG16, and the best machine learning model, KNN, about 3.14% and 18.24%, respectively. The results prove the validity of the proposed fruit maturity detection method and that this work can provide technical support for intelligent visual detection of selective harvesting robots.
Precision Agriculture - Effective shadow detection and shadow removal can improve the performance of fruit recognition in natural environments and provide technical support for agricultural... 相似文献
为了快速检测芒果树上的芒果,本文提出了一种基于无人机的树上绿色芒果视觉检测方法。本文采用深度学习技术,利用YOLOv2模型对无人机采集的芒果图像进行检测,首先通过无人机采集树上芒果图像,对芒果图像进行人工标记,建立芒果图像的训练集和测试集,通过试验确定训练模型的批处理量和初始学习率,并在训练模型时根据训练次数逐渐降低学习率,最终训练出来的模型在训练集上的平均精度(Mean average precision,MAP)为86.43%。通过试验,分析了包含不同果实数和不同光照条件下芒果图像的识别准确率,并设计了芒果树产量估计试验,试验结果表明:本文算法检测一幅图像的平均运行时间为0.08s,对测试集的识别准确率为90.64%,错误识别率为9.36%;对含不同果实数的图像识别准确率最高为94.55%,最低为88.05%;顺光条件下识别准确率为93.42%,逆光条件下识别准确率为87.18%;对芒果树产量估计的平均误差为12.79%。表明本文算法对自然环境下树上芒果有较好的检测效果,为农业智能化生产中果蔬产量的估计提供了视觉技术支持。 相似文献