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Yaxin Li Matthew R. Gronquist Guixia Hao Michele R. Holden Anatol Eberhard Russell A. Scott Michael A. Savka Erno Szegedi Sandor Sule Thomas J. Burr 《Physiological and Molecular Plant Pathology》2005,67(6):101
Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8–16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids. 相似文献
3.
Leslie Boerner Kathleen R Nevis Lynn S Hinckley E Scott Weber Salvatore Frasca 《Journal of veterinary diagnostic investigation》2004,16(2):145-149
On June 25, 2002, aquarium veterinarians treated a 5-year-old, male little blue penguin (Eudyptula minor) that was acutely recumbent and dull, with inappetence of 24-hour duration. The penguin died within 10 minutes of presentation despite emergency resuscitation efforts. Gross pathologic findings consisted of pulmonary congestion and intestinal hemorrhage. Histopathologic findings included necrosis of tips of intestinal villi, increased numbers of mononuclear cells in pulmonary interstitium and hepatic sinusoids, and gram-positive bacteria in systemic microvasculature. Transmission electron microscopic examination revealed short gram-positive bacilli located in lumina of glomerular capillaries and in cytoplasm of mononuclear phagocytic cells in the lung and liver. Erysipelothrix rhusiopathiae was recovered from the lung, liver, and intestine by bacteriologic culture. Amplicons from polymerase chain reaction (PCR) tests using Erysipelothrix genus-specific primers and total genomic DNA extracted from formalin-fixed, paraffin-embedded tissue sections of lung and intestine demonstrated 99% nucleotide sequence identity with 16S small-subunit ribosomal DNA of E. rhusiopathiae and E. tonsillarum. The source of infection was speculated to be fish in the diet; however, repeated attempts to detect Erysipelothrix spp. from the mucous layer of food fish using bacteriologic culture and PCR were unsuccessful. This is the first report of erysipelas in a captive aquatic bird. Details of the isolation of E. rhusiopathiae and the application of molecular testing to identify Erysipelothrix DNA in formalin-fixed, paraffin-embedded tissue sections are given. 相似文献
4.
Jenifer Chatfield Scott Citino Linda Munson Stanley Konopka 《Journal of zoo and wildlife medicine》2004,35(2):137-141
Historically, therapeutic monitoring for prescribed eradication treatment of Helicobacter in cheetahs (Acinonyx jubatus) with associated gastritis has been accomplished only through endoscopic biopsies. The 13C-urea breath test (UBT) can offer an alternative to repeated biopsies for therapeutic monitoring. Five male and five female cheetahs and one male Sumatran tiger (Panthera tigris) were studied. All were clinically healthy before and after this investigation. Breath samples of end-tidal expiration were taken before and after administration of a 13C-enriched urea solution through a gastroesophageal tube. Twenty-milliliter breath samples were taken at 10, 20, 30, and 40 min after administration of the urea solution. The results of the breath analysis were compared with the results of rapid urease testing, histopathologic examination, and impression smears of gastric biopsies taken at the time of the breath test. The sensitivity and specificity for the 13C-UBT in this investigation were 100%. and the positive predictive value and negative predictive value were both 100%. Although the 13C-UBT is a good noninvasive diagnostic tool for monitoring the presence of Helicobacter sp. in the gastric mucosa, endoscopy should still be used for initial diagnosis and grading of gastritis and for monitoring the progression of disease in cheetahs. The 13C-UBT is a valuable, simple, accurate, and sensitive tool for monitoring eradication of Helicobacter during therapy for clinical gastritis. 相似文献
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氯的使用可有效减少家禽胴体和屠宰加工厂的细菌。但是,我们有必要对氯有一个更好的认识和管理。 相似文献
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Ana M. Casas Carlota R. Gazulla Arantxa Monteagudo Carlos P. Cantalapiedra Marian Moralejo M. Pilar Gracia Francisco J. Ciudad William T.B. Thomas José L. Molina-Cano Scott Boden Bruno Contreras-Moreira Ernesto Igartua 《作物学报(英文版)》2021,(4):862-872
Response to vernalization and photoperiod are the main determinants controlling the time to flowering in temperate cereals. While the individual genes that dete... 相似文献
8.
P M Scott S R Kanhere 《Journal of the Association of Official Analytical Chemists》1979,62(1):141-147
PR toxin was unstable in solvent extracts of blue cheese and in strongly acidic solutions. However, it was appreciably stable over a 2.5 hr period in moderately acidic methanol-water extracts (pH 2--3) of blue cheese. Using this extraction mixture, it was determined that PR toxin was not stable in blue cheese itself. PR toxin reacted with model neutral and basic amino acids and the formation of PR imine from PR toxin in the presence of blue cheese was demonstrated. While PR imine added to blue cheese could be recovered on analysis after 5 min, over a 2--5 day period it too was unstable in the cheese. 相似文献
9.
J. A. N. Scott A. R. Procter B. J. Fergus D. A. I. Goring 《Wood Science and Technology》1969,3(1):73-92
A method has been developed for the determination of lignin distribution in the wood cell wall by ultraviolet microscopy. The method incorporates some important advances on previus applications of UV microscopy to the study of lignin distribution. Ultrathin cross-sections of wood are obtained by the sample preparation and sectioning techniques of electron microscopy. The specimens are examined in monochromatic ultraviolet light using quartz reflection optics. The microscope image is photographically recorded and the negative is subsequently subjected to densitometric analysis. Each stage of the analytical procedure has been critically assessed to determine its validity and limitations. The method is ideally suited to the study of the removal of lignin from the wood cell wall during cooking and possesses other important applications in wood technology. 相似文献
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