Habitat selection in a clonal plant

Rhizomatous growth may permit the nonrandom placement of ramets into different environments, but whether clonal plants are able to use this means to exercise adaptive habitat choice is not known. Western ragweed (Ambrosia psilostachya) plants are shown to preferentially colonize nonsaline soil over saline soil patches, and clones with the strongest preference for nonsaline soil are those least able to grow when restricted to saline conditions. In clonal plant species, nonrandom associations of genotypes with specific environments may thus reflect habitat selection by plants as well as selective mortality imposed by different habitat patches.     

Isolation and zonal fractionation of metaphase chromosomes from human diploid cells

A simple method for isolating large quantities of human metaphase chromosomes has been developed. Fractionation of chromosomes from peripheral lymphocytes on a large scale is accomplished by velocity sedimentation in an A-XII zonal rotor.     

Coherent optical control of the quantum state of a single quantum Dot

Picosecond optical excitation was used to coherently control the excitation in a single quantum dot on a time scale that is short compared with the time scale for loss of quantum coherence. The excitonic wave function was manipulated by controlling the optical phase of the two-pulse sequence through timing and polarization. Wave function engineering techniques, developed in atomic and molecular systems, were used to monitor and control a nonstationary quantum mechanical state composed of a superposition of eigenstates. The results extend the concept of coherent control in semiconductors to the limit of a single quantum system in a zero-dimensional quantum dot.     

Localization of metastable atom beams with optical standing waves: nanolithography at the heisenberg limit

The spatially dependent de-excitation of a beam of metastable argon atoms, traveling through an optical standing wave, produced a periodic array of localized metastable atoms with position and momentum spreads approaching the limit stated by the Heisenberg uncertainty principle. Silicon and silicon dioxide substrates placed in the path of the atom beam were patterned by the metastable atoms. The de-excitation of metastable atoms upon collision with the surface promoted the deposition of a carbonaceous film from a vapor-phase hydrocarbon precursor. The resulting patterns were imaged both directly and after chemical etching. Thus, quantum-mechanical steady-state atom distributions can be used for sub-0.1-micrometer lithography.     

Effects of toe angle on hoof growth and contraction in the horse

The effects of toe angle on the growth of the unshod hooves of mature horses were measured over 126 days. The hooves of 4 horses were trimmed long in the toe and short in the heel (“LT”), with toe angles of 40° for the forelimb and 50° for the hind limb hooves; 4 others were trimmed short in the toe and long in the heel (“ST”), with toe angles of 50° for the forelimb and 55° for the hind limb hooves. Growth of the hoof wall at the toe ranged between .19 and .28 mm/day, and was slowest in the forelimb hooves trimmed ST. After 126 days, the hooves trimmed LT were 7% smaller in width than they had been at day 0. Narrowing of the hoof walls and frogs was accompanied by deformation of the angles of the walls (bending outward of their weightbearing surfaces). Frog lengths and sole areas were not affected by toe angle. Regardless of trimming method, all forelimb hooves tended to return to a toe angle of 45° between trimmings, while all hind limb hooves tended toward toe angles of 52° to 53°. The soles of all hooves were basically circular in shape, although the hooves trimmed LT tended to be skewed to the left, as viewed from above, after 126 days.     

Infiltrating Foxp3+ Regulatory T cells and Histopathological Features in Canine Classical and Spermatocytic Seminomas

In humans, regulatory T (T reg) cells are known to play a critical role in both the regulation of immune homoeostasis and the progression of cancer. However, there is little information about the identification, characterization and the function of T reg cells in canine tumours. We identified T reg cells in 28 canine seminoma samples using a Forkhead box P3 (Foxp3) antibody and investigated the relationship between T reg cell infiltration and histopathological features of classical and spermatocytic seminomas (SE and SS, respectively). The Foxp3 protein showed nuclear immunostaining in infiltrating lymphocytes, and Foxp3+ cells were diffused or focally distributed in seminoma tissues. Foxp3+ cells were frequently present in the SS histotype, in seminomas that showed no evidence of tumour cell invasion into the vessels and in seminomas showing a diffuse growth pattern with three cell types. Neither the SE/SS histotype nor the histopathological features of the tumour correlated with Foxp3+ cell counts. These results indicate that Foxp3+ T reg cells may be associated with a less malignant histological phenotype or may not play a critical role in the immune response of canine seminomas. Moreover, Foxp3+ T reg cells may be associated with SS seminoma, but further studies, involving a larger number of samples, are required to better understand whether these cells play a critical role in the immune response in canine seminomas. This is the first report to demonstrate the characteristics of T reg cell infiltration in canine seminoma.     

Performance of the Giant Reed (<Emphasis Type="Italic">Arundo donax</Emphasis>) in Experimental Wetlands Receiving Variable Loads of Industrial Stormwater

Two emergent macrophytes, Arundo donax and Phragmites australis, were established in experimental subsurface flow, gravel-based constructed wetlands (CWs) and challenged by untreated stormwater collected from the hard-pan and other surfaces of a dairy processing factory in south-west Victoria, Australia. The hydraulic loading rate was tested at two levels, sequentially, 3.75 and 7.5 cm day⁻¹. Some of the monitored variables were removed more efficiently by the planted beds in comparison to unplanted CWs (biochemical oxygen demand (BOD), total nitrogen (TN) and total phosphorus (TP); p < 0.007) but there was no significant difference between the A. donax and P. australis CWs in removal of BOD, suspended solids (SS) and TN (p > 0.007) at 3.75 cm day⁻¹ or SS and TN at 7.5 cm day⁻¹. At 3.75 cm day⁻¹, BOD, SS, TN and TP removal in the A. donax and P. australis CWs was 71%, 61%, 78% and 75% and 65%, 60%, 73% and 41%, respectively. Nutrient removal at 7.5 cm day⁻¹ in the A. donax and P. australis beds was 87%, 91%, 84% and 71% and 96%, 94%, 87% and 55%, respectively. As expected, the A. donax CWs produced considerably more biomass (10 ± 1.2 kg wet weight) than the P. australis CWs (2.7 ± 1.2 kg wet weight). This equates to approximately 107 and 36 tonnes ha⁻¹ year⁻¹ biomass (dry weight) for A. donax and P. australis, respectively (assuming 250 days of growing season and single-cut harvest). The performance similarity of the A. donax- and P. australis-planted CWs indicates that either may be used in HSSF wetlands treating dairy factory stormwater, although the planting of A. donax provides additional opportunities for secondary income streams through utilisation of the biomass produced.     

Human α-defensin 6 promotes mucosal innate immunity through self-assembled peptide nanonets

Defensins are antimicrobial peptides that contribute broadly to innate immunity, including protection of mucosal tissues. Human α-defensin (HD) 6 is highly expressed by secretory Paneth cells of the small intestine. However, in contrast to the other defensins, it lacks appreciable bactericidal activity. Nevertheless, we report here that HD6 affords protection against invasion by enteric bacterial pathogens in vitro and in vivo. After stochastic binding to bacterial surface proteins, HD6 undergoes ordered self-assembly to form fibrils and nanonets that surround and entangle bacteria. This self-assembly mechanism occurs in vivo, requires histidine-27, and is consistent with x-ray crystallography data. These findings support a key role for HD6 in protecting the small intestine against invasion by diverse enteric pathogens and may explain the conservation of HD6 throughout Hominidae evolution.