Gas exchange and water-use efficiency of cv. Sangiovese grafted to rootstocks of varying water-deficit tolerance

Testing of new rootstocks for drought tolerance targets traditionally rain-fed districts where supplemental irrigation is more frequently needed due to the pressures of global warming. A seasonal evaluation of gas exchange and water-use efficiency (WUE) of cv. Sangiovese grafted to the new drought-tolerant genotype M4 in a dry-down trial against the commercial SO4 stock is reported. The experiment was conducted in 2014 on twelve 2-year-old, non-fruiting potted Sangiovese grapevines grafted on M4 and SO4 stocks and assigned to SO4-WW (well-watered), SO4-WS (water-stressed), M4-WW and M4-WS treatments. Progressive water deficit was imposed by reducing water supply to 70, 50 and 30 % of whole-canopy demand derived from concurrent measurements of transpiration in WW. Unlike SO4, M4 showed slower stress progression, as highlighted by pre-dawn leaf water potential, and retained higher whole-canopy net CO₂ exchange rates (NCER) and transpiration rates per unit of leaf area at all replenishment levels as well as exhibiting higher canopy WUE at both 50 and 30 % WW. Although single-leaf assessment was in partial disagreement with data recorded on the whole-canopy basis, robust data were acquired to confirm that the M4 stock performs better than SO4 at any water replenishment level in terms of higher NCER/leaf area and/or canopy WUE. Findings feed expectations that grafting Sangiovese to the M4 rootstock should result in a problem-solving tool for rain-fed areas subject to temporary summer drought.     

Evaluation of an open portable chamber system for measuring cover crop water use in a vineyard and comparison with a mini-lysimeter approach

Cover crops are largely used in viticultural areas because of the many positive agronomic and environmental benefits they provide. However, there is insufficient data describing the amount of water they use. A portable chamber used as an open measurement system and its suitability to measure the cover crop evapotranspiration (E) in a vineyard are described in this study. The performance of the chamber was tested by a calibration experiment (R² = 0.97). The lowest air flow rate used (9.2 l s⁻¹) was found to be suitable to limit the chamber from overheating beyond 3.1 K above the outside temperature. Furthermore, an experiment was designed to compare the daily cover crop (Festuca arundinacea var. barfelix) water use measured by the chamber system with measurements using a mini-lysimeter (ML) method and with estimates using the FAO-56 PM equation (Eo). The experiment was carried out in one inter row of a vineyard over the course of 4 days following an irrigation event. Although the field experiment was limited to 4 days, the results obtained together with the calibration trials support the possibility of the chamber being a useful tool for measuring the cover crop E. The ability of the MLs to represent the water use of the cover crop in the rest of the vineyard was limited to the first two days of the experiment, after which time the soil water content inside the containers was significantly (p = 0.007, p = 0.03) lower than in the surrounding field.     

Effects of intra-vineyard variability and soil heterogeneity on vine performance,dry matter and nutrient partitioning

Three vigor zones, identified in a Barbera vineyard by remote sensing at full canopy, were carefully ground-truthed to determine, over 2 years, the relative weight of soil factors in affecting within-field variability, and to investigate vigor zone influence on dry matter (DM) and nutrient partitioning into different vine organs. Regardless of season, high vigor (HV) achieved stronger vine capacity as total vegetative growth and yield while resulting in markedly less ripened fruits than low vigor (LV) vines. PCA analysis carried out on ten different soil and vine variables clearly separated the three vigor levels and the correlation matrix highlighted that the factors mostly contributing to HV were soil depth, soil K and P concentration, total available water, clay fraction and N_leaf concentration. Conversely, sand fraction was the main marker for LV. When annual DM retrieved in clusters, canes, leaves, and shoot clippings was calculated for each vigor level and expressed as content (i.e. kg/ha) there was a general decreasing trend moving from HV to LV. However, when DM partitioned to each organ was given on a relative basis (i.e. percentage over total) results were similar across vigor levels. Similarly, when nutrients were given as content (e.g. kg or g/ha) out of 120 within-vigor combinations (12 nutrients, 2 seasons, 5 organs), 65 showed a significant difference between HV and LV. Conversely, with data expressed on a concentration basis (i.e. % DM) the number of significant differences between the vigor level means fell to 15. The study strengthens the causal link between soil properties and intra-vineyard spatial variability and clarifies that patterns of dry matter and nutrient partitioning to different vine organs are mildly affected by vine vigor when referred on a relative basis.

     

Impact of climate change on the possible expansion of almond cultivation area pole-ward: a case study of Abruzzo,Italy

Climate warming is causing an advance of the latest spring frosts and a consequent decrease of spring freeze risk during flowering. Cultivation areas of early blooming tree crops, such as almond, could be shifted pole-ward as consequence of global warming. On the other hand, warming winters and springs can cause an advance of the flowering period. The aim of the present work was to estimate which was the impact of climate change in the past six decades on the spring freeze damage risk during the almond blooming period in the Abruzzo region of Italy. According to our analysis a reduction of spring freeze risk due to the mitigation of springtime temperatures was counterbalanced by advanced almond blooming leaving the risk of spring freeze damage unchanged. These results suggest that the adaptation of almond phenology to changing climates could continue to limit the northward expansion of almond in Italy. Finally, these results may suggest that the loss of suitable areas due to loss of chilling units in the warmest climate areas cannot be compensated for by a pole-ward shift of almond plantings.     

Response of “Sangiovese” grapevines to partial root-zone drying: Gas-exchange,growth and grape composition

Our study focuses on the physiological response and yield-quality performance of split-root potted Sangiovese grapevines under a partial root-zone drying (PRD) regime from pre-veraison to harvest by withholding water from one of the two pots and comparing the results to a well-watered control (WW). While predawn water potential (ψ_pd) tended to equilibrate in PRD with the soil moisture level of the wet pot, both stem (ψ_st) and mid-day leaf-water potential (ψ_l) were markedly lower in PRD as compared to WW vines, indicating that Sangiovese shows anisohydric response. On the other hand, the seasonal reduction of leaf assimilation rate (A) in PRD over the 6-week stress period versus WW was 16% as compared to a 41 and 25% for leaf stomatal conductance (g_s) and transpiration (E), respectively. As a consequence, intrinsic WUE (A/g_s) was markedly increased in the half-stressed vines, suggesting a response more typical of an isohydric strategy. Shoot growth was promptly checked in PRD vines, which had no limitation in yield and better grape composition as per soluble solids and total anthocyanins. These responses occurred in spite of sub-optimal leaf photosynthesis rates and lowered leaf-to-fruit ratio and qualify Sangiovese as a good candidate for adapting to regulated deficit irrigation strategies.