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In mid-September, 1 month before the insertion of intravaginal pessaries to induce sexual activity, blood samples were collected every 4 days from 16 ewe lambs aged 7 months, in order to determine the incidence of ovulations by measurement of plasma progesterone concentrations. It has been studied whether the response to a progestagen treatment of ewe lambs apparently close to puberty could be modified by the onset of the ovarian events preceding puberty. The effect of the presence or absence of ovulations prior to progestagen treatment on the potential reproductive performance (fertility, litter size and fecundity), embryo development [embryo quality and interferon-tau (IFNτ) secretion], luteal function (progesterone secretion in vitro ) and endometrial progesterone content was studied in seven ovulating (Ov+) and nine nonovulating ewe lambs (Ov−) on day 14 after mating. The best potential reproductive results were obtained with Ov+ animals, although these differences could not be initially attributed to either different progesterone secretion in vitro or concentration of endometrial progesterone. Irrespective of the experimental groups, secretion of progesterone by luteal tissue from ewe lambs with normal embryos was significantly greater (p<0.05) than that of animals with abnormal embryos or with no embryos. Normal embryos secreted a higher amount of IFN-τ than those embryos classified as abnormal (p<0.07). In conclusion, ewe lambs which exhibit luteal activity before puberty have the highest levels of reproductive performances after a progestagen treatment. Corpora lutea from ewe lambs with normal embryos had higher rates of progesterone secretion in vitro and their embryos had a higher IFN-τ production by the embryos, indicating greater capacity for subsequent development. 相似文献
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Bull sperm pellets experimentally infected with various concentrations of Mycoplasma (M.) bovis, M. bovigenitalium, and M. californicum were stored several months at -196 degrees C and were subsequently tested for mycoplasmas, using different methods and culturing media. M. bovis was reliably identified by laboratory diagnosis up to a concentration of 10(2) cfu/pellet. M. bovigenitalium and M. californicum were found to make higher demands on the quality of culturing substrates. Recommended are 1-h broth incubation and 5-d broth breeding. 相似文献
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H Pfützner 《Archiv fuer experimentelle veterinaermedizin》1989,43(5):697-698
Mycoplasma bovis antiserum "Dessau" was used to investigate 4,553 mycoplasma strains, between 1985 and 1987, using a method described elsewhere. This diagnostic Agent is successfully used at present at all Regional Institutes of Veterinary Services throughout the GDR. 相似文献
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Herbicide translocation was investigated in a Haplic Luvisol from loess (clayey silt; Klein-Altendorf near Bonn, Germany) and a Eutric Cambisol (sandy loam; fluvial sediments on the lower Rhine terrace north of Bonn) using the herbicide diuron as an example. diuron was applied once a year in May at the recommended dose of 2.4 kg/ha. It rapidly degraded in the topsoil: the “time for 50% disappearance” (DT50) was 14–30 days. A population of adapted soil micro-organisms, which is repeatedly exposed to applications of the same compound every year, is thought to be responsible for this fast degradation. Remaining Diuron residues were largely degraded in the loess soil during the subsequent summer and became less mobile due to increasing binding strength. As a result, residue levels and leaching in the loess soil during the winter were negligible, with diuron traces of less than 10 µg/kg below the topsoil 11 months after application. Comparison of the translocation in a sandy loam soil showed—as expected—that diuron was leached to the subsoil to a higher extent than in clayey silt (loess). However, in the loess soil, diuron leached to the subsoil via macropores after 20 mm of natural precipitation within 1 week after herbicide application due to macropore flow. Colour tracer and analysis of the linings of earthworm burrows identified these macropores as preferred pathways of herbicide leaching. Macropore flow occurred in wet soil and moist soil with a sealed surface or crust but not in dry soil. Superficial mechanical soil cultivation before the herbicide application is discussed as one possibility to interrupt macropore entries and reduce herbicide leaching after its application in the spring. 相似文献
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U. Merz V. Martinez R. Schwärzel 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):71-77
Powdery scab of potato, once established in a field, is difficult to control because of the longevity of the resting spores (cystosori) of the causal organism, Spongospora subterranea f.sp. subterranea. Host resistance is likely to be the most efficient in a long-term control strategy for preventing build-up of field inoculum and spread of the disease. Resistance screening of potato cultivars is mostly done in laborious field trials where disease development is likely to be unpredictable. A bioassay with potato tissue cultured plantlets and cystosori as inoculum is described and was tested for its potential to screen potato cultivars at an early stage for their relative susceptibility to powdery scab by comparing the lab results with field data. With cystosori inoculum of Swiss origin, the laboratory test showed clear differences between the potato cultivars in the severity of zoosporangial root infection which correlated better with ranked tuber infection data, compared to root galling. There are apparent differences in the relative trends in susceptibility between roots and tubers of five selected cultivars when using naturally infested soil instead of prepared cystosori as inoculum in the lab bioassay. Furthermore, differences in the severity of zoosporangial root infection of two selected cultivars were found when cystosori from different countries where used as inoculum. A possible host genotype × pathogen interaction is discussed. The bioassay has the potential to screen and select for resistant material at an early breeding stage thus making field trials not unnecessary but more economical. It will allow the use of a standard set of pathogen collections and facilitate testing for inoculum virulence in infested soils. 相似文献
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J. Schäler R. Wellmann J. Bennewitz G. Thaller D. Hinrichs 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(2):63-72
Recovering native uniqueness has major importance for breeds with historic introgression. The aim of the study was to estimate population genetic parameters for two local red cattle breeds from Northern Germany and to study possibilities to reverse introgression. Genealogical information consisted of 90,783 individuals for German Angler and 187,255 individuals for Red Dual-Purpose cattle breed, with additional information on sex, born, breed, status, and conventional breeding values. It is concluded that the native genetic contribution could be included as an additional trait in the total merit index in order to recover a part of the native genetic background. Native contributions should be estimated in the long term from marker data in order to account for Mendelian sampling. The maintenance of a sufficient genetic diversity of native alleles can be achieved by an advanced OCS with appropriate constraints. 相似文献